Age-related differences in the normal human cornea: a laser scanning in vivo confocal microscopy study

Niederer, Rachael L; Perumal, Divya; Sherwin, Trevor; McGhee, Charles N J

doi:10.1136/bjo.2006.112656

Cited by 229 publications

(184 citation statements)

References 27 publications

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“…Moreover, the morphology of corneal blood vessels has been well studied and described both in vivo and ex vivo, mainly in the last decade, by means of confocal microscopy [34][35][36] or other innovative techniques [37,38]. Our present results are in agreement with all these previous observations and add new evidence on the morphological development of corneal neovascularization.…”

Section: Discussionsupporting

confidence: 83%

Neovascularization in Alkali-Burned Rabbit Cornea

Pescosolido¹

2015

J Clin Exp Ophthalmol

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Objective: To study the neovascularization in regenerating and proliferating corneal cells following a standard alkali injury in rabbit eye. Methods:Three and six weeks after the creation of an alkali burn in the center of the right cornea of six albino rabbits, the animals were killed and histological sections from the cornea of both eyes were stained, photographed and studied for a possible formation of a neovascularization. The photographs were examined using the Quantimet image analyzer (Leica) and statistical analysis of the data was performed.Results: Sections of the injured cornea showed the formation of neovessels in the epithelial and superficial stromal layers. The neovascularization is present after 3 weeks of the corneal injury. After 6 weeks from the corneal alkali burn, neovessels are increased. Conclusions:There is a growing body of evidence suggesting that vascular abnormalities may play a crucial role in several ocular diseases. To improve our knowledge of the vascular involvement in these conditions, there is a need for a non-invasive imaging modality capable of assessing microcirculation within ocular tissue beds both in vitro and in vivo. This study shows that ultra-high sensitive optical microangiography, associated with other experimental techniques, is an adequate technique to visualize the eye surface microcirculations and to quantify microvascular vessel density under both normal and physio-pathological conditions.

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Section: Discussionsupporting

confidence: 83%

Neovascularization in Alkali-Burned Rabbit Cornea

Pescosolido¹

2015

J Clin Exp Ophthalmol

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“…In some of the earlier studies, age had no significant effect on CCT (16)(17)(18)(19)(20)(21)(22). All these reports postulated that the number and race of the subjects, and differences in pachymeter instruments, might affect the results (10).…”

Section: Discussionmentioning

confidence: 99%

Age-related changes in biomechanical parameters of the cornea and intraocular pressure in a healthy Turkish population

Şen¹,

Elgin²,

Yüksekkaya³

et al. 2014

Turk J Med Sci

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“…In the present study, the densities of BECs in normal eyes were similar compared with previous reports. [15][16][17][18] There are no significant differences between normal eyes and injured eyes concerning the densities of BECs, at 3 months after injury, except those with grade IV injury. Our finding suggested that the densities of BECs might be relatively stable during the epithelial recovery after chemical injury.…”

Section: Discussionmentioning

confidence: 94%

In vivo confocal microscopy of early corneal epithelial recovery in patients with chemical injury

Xiang

et al. 2015

Eye

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Purpose To investigate the early recovery of corneal epithelium in patients with chemical injuries by the application of in vivo laser scanning confocal microscopy (LSCM). Methods LSCM was performed on 26 eyes of 18 consecutive patients at 1, 2, and 3 months post injury. The morphology of central corneal epithelium and limbus was evaluated. Analysis was performed to compare the densities of corneal apical surface cells (ASCs) and basal epithelial cells (BECs) among different injury severity and time points after injury. The comparisons were also made on inflammatory cells (ICs) and dendritic cells (DCs) infiltrating at the limbus. Moreover, the presence rate of palisades of Vogt (POV) was analyzed at 3 months post injury. Results Corneal re-epithelialization was found within 3 months post injury in all eyes, except that persistent epithelium defect was identified in grade IV injured eyes even at 3 months after injury. The injury severity had a significantly negative correlation with the densities of ASCs. However, it had no relationship with the densities of BECs. The density of ICs at the limbus decreased significantly with the prolongation of follow-up, except in the grade I injured eyes. The presence rate of POV also had a significantly negative correlation with injury severity.Conclusion Epithelial recovery after chemical injury varied in cellular morphology and the densities of ASCs among eyes with different injury severity. The presence rates of POV decreased with the aggravation of injury. LSCM is a feasible method for observing the early recovery of corneal epithelium in patients with chemical injuries.

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Age-related differences in the normal human cornea: a laser scanning in vivo confocal microscopy study

Cited by 229 publications

References 27 publications

Neovascularization in Alkali-Burned Rabbit Cornea

Neovascularization in Alkali-Burned Rabbit Cornea

Age-related changes in biomechanical parameters of the cornea and intraocular pressure in a healthy Turkish population

In vivo confocal microscopy of early corneal epithelial recovery in patients with chemical injury

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