Age-Related Differences in Rat Multipotent Mesenchymal Stromal Bone Marrow Cells

Valyushina, M. P.; Буравкова, Л. Б.

doi:10.1007/s10517-013-2097-1

Cited by 6 publications

(7 citation statements)

References 13 publications

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“…Certainly, when it comes to maintaining bone mass, age and gender differences are well recognized. Moreover, past work has suggested that differences in bone loss between genders or during aging can be correlated with the abundance and senescence of skeletal progenitors [20, 52, 53]. Associated with our data, CTGF conditional loss of function studies have reported variable impacts on bone volume associated with animal age and gender.…”

Section: Discussionsupporting

confidence: 84%

Connective Tissue Growth Factor reporter mice label a subpopulation of mesenchymal progenitor cells that reside in the trabecular bone region

Wang

Strecker

Liu

et al. 2015

Bone

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Few gene markers selectively identify mesenchymal progenitor cells inside the bone marrow. We have investigated a cell population located in the mouse bone marrow labeled by Connective Tissue Growth Factor reporter expression (CTGF-EGFP). Bone marrow flushed from CTGF reporter mice yielded an EGFP+ stromal cell population. Interestingly, the percentage of stromal cells retaining CTGF reporter expression decreased with age in vivo and was half the frequency in females compared to males. In culture, CTGF reporter expression and endogenous CTGF expression marked the same cell types as those labeled using Twist2-Cre and Osterix-Cre fate mapping approaches, which previously has been shown to identify mesenchymal progenitors in vitro. Consistent with this past work, sorted CTGF+ cells displayed an ability to differentiate into osteoblasts, chondrocytes, and adipocytes in vitro and into osteoblast, adipocyte, and stromal cell lineages after transplantation into a parietal bone defect. In vivo examination of CTGF reporter expression in bone tissue sections revealed it marked cells highly localized to the trabecular bone region and was not expressed in the perichondrium or periosteum. Mesenchymal cells retaining high CTGF reporter expression were adjacent to, but distinct from mature osteoblasts lining bone surfaces and endothelial cells forming the vascular sinuses. Comparison of CTGF and Osterix reporter expression in bone tissue sections indicated an inverse correlation between the strength of CTGF expression and osteoblast maturation. Down-regulation of CTGF reporter expression also occurred during in vitro osteogenic differentiation. Collectively, our studies indicate that CTGF reporter mice selectively identify a subpopulation of bone marrow mesenchymal progenitor cells that reside in the trabecular bone region.

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Section: Discussionsupporting

confidence: 84%

Connective Tissue Growth Factor reporter mice label a subpopulation of mesenchymal progenitor cells that reside in the trabecular bone region

Wang

Strecker

Liu

et al. 2015

Bone

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“…22 It was further found that BMSCs from Wistar rats aged < 1 month old had a doubling time of 26.07 § 1.81 hours and a doubling number of 3.64 § 0.19 while rats aged > 12 months old had a doubling time of 32.20 § 3.89 hours and a doubling number of 3.07 § 0.18, suggesting that the young BMSCs replicated more quickly and to a greater degree than did the old BMSCs. 23 This phenomenon was also observed in rhesus macaques where BMSCs from young monkeys had more rapid proliferation rates than those from older monkeys. 6 The above animal studies have counterparts in human tissue research.…”

Section: Donor Age Dependent Cell Senescencementioning

confidence: 72%

Age associated communication between cells and matrix: a potential impact on stem cell-based tissue regeneration strategies

Lynch

Pei²

2014

Organogenesis

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A recent paper demonstrated that decellularized extracellular matrix (DECM) deposited by synovium-derived stem cells (SDSCs), especially from fetal donors, could rejuvenate human adult SDSCs in both proliferation and chondrogenic potential, in which expanded cells and corresponding culture substrate (such as DECM) were found to share a mutual reaction in both elasticity and protein profiles (see ref.

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“…According to the current data, reduction in O 2 concentration to the tissue‐related level in cellular microenvironment (“physiologic” hypoxia) can affect the features of various tissue‐derived MSCs . Recently, we have demonstrated an increase in the proliferative activity and CFU‐F number of adipose tissue‐derived stromal cells (ASCs), while, on the contrary, the adipogenic and osteogenic differentiation decelerated . Global microarray analysis of 22 184 genes in ASCs expanded at 5% O 2 revealed the differential gene expression of 558 genes in comparison with ASCs at 20% O 2 .…”

Section: Introductionmentioning

confidence: 88%

“…14,15 Recently, we have demonstrated an increase in the proliferative activity and CFU-F number of adipose tissue-derived stromal cells (ASCs), while, on the contrary, the adipogenic and osteogenic differentiation decelerated. [16][17][18] Global microarray analysis of 22 184 genes in ASCs expanded at 5% O 2 revealed the differential gene expression of 558 genes in comparison with ASCs at 20% O 2 . Wherein, the expression of genes involved in proliferation, cell metabolism, and signaling was upregulated, while that of the ones responsible for the connective tissue matrix and cellular cytoskeleton components decreased.…”

Section: Introductionmentioning

confidence: 99%

“…Evaluation of size and granularity of adipose tissue-derived stromal cells (ASCs) at early (2-5) and late passages(14)(15)(16)(17)(18)(19)(20): A, representative flow cytometry density plots of forward scattering vs side scattering (FSC-A/SSC-A) to illustrate size/structure of ASCs; B, average ASC size, C, average ASC granularity. Data are shown as mean ± standard deviation (*P < .01).…”

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confidence: 99%

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Expansion of adipose tissue‐derived stromal cells at “physiologic” hypoxia attenuates replicative senescence

Ratushnyy

Lobanova

Буравкова

2017

Cell Biochemistry & Function

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Multipotent mesenchymal stromal cells are considered as a perspective tool in cell therapy and regenerative medicine. Unfortunately, autologous cell therapy does not always provide positive outcomes in elder donors, perhaps as a result of the alterations of stem cell compartments. The mechanisms of stem and progenitor cell senescence and the factors engaged are investigated intensively. In present paper, we elucidated the effects of tissue-related O on morphology, functions, and transcriptomic profile of adipose tissue-derived stromal cells (ASCs) in replicative senescence in vitro model. Replicatively senescent ASCs at ambient (20%) O (12-21 passages) demonstrated an increased average cell size, granularity, reactive oxygen species level, including anion superoxide, lysosomal compartment activity, and IL-6 production. Decreased ASC viability and proliferation, as well as the change of more than 10 senescence-associated gene expression were detected (IGF1, CDKN1C, ID1, CCND1, etc). Long-term ASC expansion at low O (5%) revoked in part the replicative senescence-associated alterations.

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Age-Related Differences in Rat Multipotent Mesenchymal Stromal Bone Marrow Cells

Cited by 6 publications

References 13 publications

Connective Tissue Growth Factor reporter mice label a subpopulation of mesenchymal progenitor cells that reside in the trabecular bone region

Connective Tissue Growth Factor reporter mice label a subpopulation of mesenchymal progenitor cells that reside in the trabecular bone region

Age associated communication between cells and matrix: a potential impact on stem cell-based tissue regeneration strategies

Expansion of adipose tissue‐derived stromal cells at “physiologic” hypoxia attenuates replicative senescence

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