Age-related decline in the expression of GDF9 and BMP15 genes in follicle fluid and granulosa cells derived from poor ovarian responders

Gong, Yan; Li‐Ling, Jesse; Xiong, Dongsheng; Wei, Jiajing; Zhong, Taiqing; Tan, Hao

doi:10.1186/s13048-020-00757-x

Cited by 25 publications

(13 citation statements)

References 41 publications

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“…Down-regulating GDF9 can induce intraovarian hyperandrogenism and finally lead to follicular arrest [28]. A lower quality of oocytes in patients with a poor ovarian response correlated with a decreased expression of GDF9 and BMP15 in follicular fluid (FF) and granulosa cells (GCs) [29]. In this study, the expression of maternal genes was analyzed using RNA-seq, and the expression of GDF9 and BMP15 was further tested by qRT-PCR.…”

Section: Discussionmentioning

confidence: 99%

Single-Cell RNA-Seq Revealed the Gene Expression Pattern during the In Vitro Maturation of Donkey Oocytes

Li¹,

Song²,

Yin

et al. 2021

Genes

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Donkeys are an important domesticated animal, providing labor, meat, milk, and medicinal materials for humans. However, the donkey population is continuously declining and even at risk of extinction. The application of modern animal production technology, such as oocyte in vitro maturation, is a promising method to improve the donkey population. In this study, we explore the gene expression patterns of donkey germinal vesicle (GV) and in vitro matured metaphase II (MII) oocytes using single cell RNA-seq of the candidate genes along with the regulatory mechanisms that affect donkey oocyte maturation. We identified a total of 24,164 oocyte genes of which 9073 were significant differentially expressed in the GV and MII oocytes. Further Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis indicated that these genes were associated with the meiotic cell cycle, mitochondrion activity, and N-glycan biosynthesis, which might be the key genes and regulatory mechanisms affecting the maturation of donkey oocytes. Our study provides considerable understanding regarding the maturation of donkey oocytes and serves as a theoretical basis for improving the development of donkey oocytes, which could ultimately benefit the expansion of the donkey population and conservation of biodiversity and genetic resources.

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Section: Discussionmentioning

confidence: 99%

Single-Cell RNA-Seq Revealed the Gene Expression Pattern during the In Vitro Maturation of Donkey Oocytes

Li¹,

Song²,

Yin

et al. 2021

Genes

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“…Considering the significant role of adiponectin in counteracting inflammatory processes and increasing the sensitivity of tissues to insulin, this adipokine may be considered as a potential drug [ 147 , 290 ]. Exogenous adiponectin supplementation in early pregnant individuals may help to avoid the metabolic syndrome of adult female offspring, according to the recent studies on a mouse PCOS model [ 294 ]. A probable mechanism underlying this effect is related to the activation of the AMPK/PI3K-Akt signaling pathway in PCOS offspring mice [ 294 ].…”

Section: Discussionmentioning

confidence: 99%

“…Exogenous adiponectin supplementation in early pregnant individuals may help to avoid the metabolic syndrome of adult female offspring, according to the recent studies on a mouse PCOS model [ 294 ]. A probable mechanism underlying this effect is related to the activation of the AMPK/PI3K-Akt signaling pathway in PCOS offspring mice [ 294 ].…”

Section: Discussionmentioning

confidence: 99%

Recent Update on the Molecular Mechanisms of Gonadal Steroids Action in Adipose Tissue

Wawrzkiewicz-Jałowiecka

Lalik

Soveral

2021

IJMS

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The gonadal steroids, including androgens, estrogens and progestogens, are involved in the control of body fat distribution in humans. Nevertheless, not only the size and localization of the fat depots depend on the sex steroids levels, but they can also highly affect the functioning of adipose tissue. Namely, the gonadocorticoids can directly influence insulin signaling, lipid metabolism, fatty acid uptake and adipokine production. They may also alter energy balance and glucose homeostasis in adipocytes in an indirect way, e.g., by changing the expression level of aquaglyceroporins. This work presents the recent advances in understanding the molecular mechanism of how the gonadal steroids influence the functioning of adipose tissue leading to a set of detrimental metabolic consequences. Special attention is given here to highlighting the sexual dimorphism of adipocyte functioning in terms of health and disease. Particularly, we discuss the molecular background of metabolic disturbances occurring in consequence of hormonal imbalance which is characteristic of some common endocrinopathies such as the polycystic ovary syndrome. From this perspective, we highlight the potential drug targets and the active substances which can be used in personalized sex-specific management of metabolic diseases, in accord with the patient’s hormonal status.

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“…For poor responders over 35 years, the rates of implantation and clinical pregnancy were lower than those of under 35. Meanwhile, younger poor responders (<35) still have a reasonable number of transferable embryos (2.02 ± 0.57) and an acceptable pregnancy rate (37.50%) (32). It is well understood that increased age leads to a reduction in the quantity and quality of oocytes, which is accompanied by a decline in female fertility (33,34).…”

Section: Discussionmentioning

confidence: 99%

Development and Validation of a Clinical Pregnancy Failure Prediction Model for Poor Ovarian Responders During IVF/ICSI

Zeng

et al. 2021

Front. Endocrinol.

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BackgroundsDespite the great advances in assisted reproductive technology (ART), poor ovarian response (POR) is still one of the most challenging tasks in reproductive medicine. This predictive model we developed aims to predict the individual probability of clinical pregnancy failure for poor ovarian responders (PORs) under in vitro fertilization/intracytoplasmic sperm injection (IVF/ICSI).MethodsThe nomogram was developed in 281 patients with POR according to the Bologna criteria from January 2016 to December 2019, with 179 in the training group and 102 in the validation group. Univariate and multivariate logistic regression analyses were used to identify characteristics that were associated with clinical pregnancy failure. The nomogram was constructed based on regression coefficients. Performance was evaluated using both calibration and discrimination.ResultsAge >35 years, body mass index (BMI) >24 kg/m2, basic follicle-stimulating hormone (FSH) >10 mIU/ml, basic E2 >60 pg/ml, type B or C of endometrium on human chorionic gonadotropin (hCG) day, and the number of high-quality embryos <2 were associated with pregnancy failure of POR patients. The area under the receiver operating characteristic curve (AUC) of the training set is 0.786 (95% confidence interval (CI): 0.710–0.861), and AUC in the validation set is 0.748 (95% CI: 0.668–0.827), showing a satisfactory goodness of fit and discrimination ability in this nomogram.ConclusionOur nomogram can predict the probability of clinical pregnancy failure in PORs before embryo transfer in IVF/ICSI procedure, to help practitioners make appropriate clinical decisions and to help infertile couples manage their expectations.

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Age-related decline in the expression of GDF9 and BMP15 genes in follicle fluid and granulosa cells derived from poor ovarian responders

Cited by 25 publications

References 41 publications

Single-Cell RNA-Seq Revealed the Gene Expression Pattern during the In Vitro Maturation of Donkey Oocytes

Single-Cell RNA-Seq Revealed the Gene Expression Pattern during the In Vitro Maturation of Donkey Oocytes

Recent Update on the Molecular Mechanisms of Gonadal Steroids Action in Adipose Tissue

Development and Validation of a Clinical Pregnancy Failure Prediction Model for Poor Ovarian Responders During IVF/ICSI

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