Age-related decline in protein synthesis in the rat parotid gland

Kim, S. K.; Weinhold, Paul A.; Han, Seong S.; Wagner, D. J.

doi:10.1016/0531-5565(80)90078-9

Cited by 35 publications

(27 citation statements)

References 31 publications

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“…As we have described previously (Kim et al, 1980;Kim, 1981), fat, which is first detected in the glands of 12-month-old rats, increases in amounts with age. However, the age-related differences that involve the secretory acini are less obvious at this level.…”

Section: Observationsmentioning

confidence: 64%

“…Therefore, it is unlikely that the increasing number of lipofuscin granules affects the cell function by replacing other cytoplasmic organelles. For example, the rate of protein synthesis in rat parotid glands declines by about 50% between the ages of 2 and 30 months (Kim et al, 1980). Thus, it seems unlikely that the mere presence of lipofuscin granules affects protein synthesis to this degree.…”

Section: Discussionmentioning

confidence: 97%

“…The parotid glands were removed, sliced into smaller pieces, and further fixed in the same fixative for another hour. The tissues were postfixed in Os04, and prepared for electron microscopy as previously described (Kim et al, 1980;Kim, 1981).…”

Section: Methodsmentioning

confidence: 99%

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Changes in the secretory acinar cells of the rat parotid gland during aging

Kim

1984

Anat. Rec.

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The secretory acinar cells of parotid glands from rats of varying ages have been examined by electron microscopy to determine what age-related changes occur in these cells. The most prominent change noted in these cells is the progressive increase in the amount of lipofuscin granules with age. Lipofuscin granules are membrane-bound structures consisting of lipids, other subcomponents, and a matrix. In addition, these cells contain lipid droplets that are not associated with any other components and tend to accumulate at the base of the cells in older rats. Also, many acinar cells in the glands of old rats contain altered secretory granules which appear to be in the process of degeneration. The accumulation of lipid and degenerating secretory granules appears to be related to the reduced level of cellular secretory activity in the glands of older rats. It is possible that these two types of inclusions contribute to the formation of lipofuscin granules. Lipofuscin and degenerating secretory granules are associated with acid phosphatase, which is demonstrated cytochemically, indicating that these granules are lysosomal structures.

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Section: Observationsmentioning

confidence: 64%

Section: Discussionmentioning

confidence: 97%

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Changes in the secretory acinar cells of the rat parotid gland during aging

Kim

1984

Anat. Rec.

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“…-The aamylase activity is highest in the glands of 12-month-old rats, and it is reduced significantly in older (18,24, and 30 mo) rats (Table 1). It appears that the glandular level of a-amylase activity does not change significantly with age after it reaches the low level at 18 mo.…”

Section: Resultsmentioning

confidence: 94%

“…A decline in protein synthesis has been demonstrated in prostate,5 liver,6-9 brain, 0-12 muscle,13,14 testis, 5 738 and lens.16 We have also reported previously that the synthesis of proteins declines with aging in parotid salivary glands. [17][18][19] To determine whether the cellular content of secretory proteins decreases with the reduction in protein synthesis in salivary glands, the differences in the level of aamylase activity and the incorporation of 3H-leucine into acid-insoluble proteins have been compared in parotid glands of rats of varying ages. The rat parotid gland provides an excellent system for this kind of study since the gland consists primarily of one type of secretory cell which produces a purely proteinaceous secretion consisting largely of a-amylase.…”

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confidence: 99%

Age-related Changes in the Cellular Level of Amylase and Protein Synthesis in the Rat Parotid Gland

Kim

1981

J Dent Res

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Age-related changes in the cellular content of secretory proteins and protein synthesis were studied in parotid glands of rats of various ages. The secretory protein content (determined by measuring the level of alpha-amylase activity) and the synthesis of proteins (assayed by the rate of incorporation of 3H-leucine into acid-insoluble proteins) decline with increasing age. Morphological and radioautographic studies of the gland indicate that the decline in protein synthesis is due to the reduction in the ability of secretory cells to synthesize proteins.

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Changes with senescence in the fine structure of the granular convoluted tubule of the submandibular gland of the mouse

Gresik

1989

Am. J. Anat.

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Cells of the granular convoluted tubules (GCTs) of the submandibular gland of senescent male mice show structural changes indicative of functional decline. In order to define the nature of these age-related changes more clearly, the fine structure of GCT cells of 12- and 28-month-old males was compared. In old mice, there was cell-to-cell variation in the extent of these changes, with some cells of senescent males appearing no different from those of young adults. In affected cells the most striking alterations were seen in secretion granules and lysosomal elements. Secretion granules varied greatly in size, with some GCT cells having only very fine apical granules. Secondary lysosomes and large lipofuscin granules were frequent in the basal cytoplasm. Very large dense bodies (3-5 micron) occurred in many cells. These possibly represent intracellular pools of released secretory materials, as they were occasionally seen in continuity with the luminal contents. Structures whose appearance was intermediate between the very large dense bodies and lipofuscin granules were common, suggesting crinophagic activity. There was an apparent decrease in numbers of polysomes and in the extent of the Golgi apparatus. These fine structural changes are consistent with impairments with advanced age in synthesis and posttranslational processing of secretory products by affected GCT cells. In addition to cell-to-cell variation in any one male, there was also interanimal variation in the degree and extent of these senescent changes.

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Age-related decline in protein synthesis in the rat parotid gland

Cited by 35 publications

References 31 publications

Changes in the secretory acinar cells of the rat parotid gland during aging

Changes in the secretory acinar cells of the rat parotid gland during aging

Age-related Changes in the Cellular Level of Amylase and Protein Synthesis in the Rat Parotid Gland

Changes with senescence in the fine structure of the granular convoluted tubule of the submandibular gland of the mouse

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