Age‐related changes of the endogenous cardiolipin and plasmalogens of guinea pig kidney and their in vitro hydrolysis by endogenous phospholipases: a thin layer chromatographic analysis in conjunction with densitometric measurement

Abstract

The phosphoglycerides profile of guinea pig kidney, fetal, young adult, and aged, and their in vitro response to the endogenous lipolytic enzymes, mainly in the phospholipase group were determined by TLC technology in conjunction with densitometric measurement. Changes in phosphoglycerides profile subsequent to in vitro incubation of these tissues at pH 7.4, and 38 degrees C for 45 min and prior to phospholipid extraction has provided evidence relating to their respective lipolytic enzymes capabilities and age. These changes are mainly related to endogenous cardiolipin (CL), alkenyl phospholipids (phosphatidyl ethanolamine and phosphatidyl choline) and their endogenous deacylation to their respective lyso derivatives monolysocardiolipin (MLCL), lyso alkenyl phosphatidyl ethanolamine (LPE), and lyso alkenyl phosphatidyl choline (LPC) by endogenous phospholipases. The hydrolysis of the plasmalogen confirms the action of endogenous PLA(2) on sn-2 fatty acids of these compounds.

Mitochondria as intracellular signaling platforms in health and disease

“…For instance, cardiolipin has been shown to inhibit ceramide synthesis and promote its cleavage by ceramidase ( El Bawab et al, 2001 ; Kim et al, 2016 ; Okino et al, 2003 ). On the other hand, manipulation of ceramide modulates in vivo cardiolipin levels ( Babenko and Storozhenko, 2017 ), which is consistent with observations of an age-related accumulation of ceramide and a corresponding decline in cardiolipin ( Helmy et al, 2003 ; Monette et al, 2011 ; Petrosillo et al, 2008 ; Sen et al, 2007 ). In addition, alterations in these sphingolipid-dependent pathways have been increasingly linked to conditions such as Alzheimer’s disease ( Pera et al, 2017 ).…”

Lipidomics of familial longevity

“…Ether PC species have been found to prevent oxidation of polyunsaturated fatty acids in lipoproteins, a feature suggested as sex dependent (Helmy et al ., ; Wiesner et al ., ). Ether PC species were present in higher levels in the offspring of nonagenarians a characteristic of a youthful profile, which is consistent with their antioxidant and cardioprotective roles.…”

Age-related decline in mitochondrial bioenergetics: Does supercomplex destabilization determine lower oxidative capacity and higher superoxide production?

“…Helmy et al observed an age-related increase in the ratio of monolyso-cardiolipin to mature cardiolipin in the guinea pig kidney [97], an indicator of defective incorporation of acyl chain units into cardiolipin during the remodeling cycle [98]. In addition, tetralinoleoyl-cardiolipin ([18:2] 4 -cardiolipin), the predominant species in the heart [61, 99], appears to be the most adversely affected on an age basis [100].…”