Age-related changes of antioxidant enzyme activities, glutathione status and lipid peroxidation in rat erythrocytes after heat stress

Öztürk, Oğuz; Gümüşlü, Saadet

doi:10.1016/j.lfs.2004.03.020

Cited by 47 publications

(9 citation statements)

References 40 publications

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“…Reduced form of GSH is the most abundant nonprotein thiol present in the cell. It can nonenzymatically reduce substances, such as peroxides or free radicals, which accumulate in cells under oxidizing conditions (Bukowska and Kowalska,; Öztürk and Gümülü,). By maintaining the intracellular reducing environment, GSH prevents intracellular protein thiols from oxidizing to disulfides.…”

Section: Discussionmentioning

confidence: 99%

Silymarin prevents the toxicity induced by benzo(a)pyrene in human erythrocytes by preserving its membrane integrity: Anin vitrostudy

2011

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Silymarin, the purified extract from milk thistle Silybum marianum (L.) Gaertn, consists mainly of four isomeric flavonolignans: silibinin, isosilibinin, silidianin, and silichristin. The present study was carried out to evaluate the protective potential of silymarin in human erythrocytes against in vitro exposure to the carcinogen benzo(a)pyrene (B(a)P). Erythrocytes isolated from human blood were divided into four groups and treated with Vehicle [Group I], B(a)P (300 μM) [Group II], Silymarin (500 μM) + B(a)P (300 μM) [Group III], and Silymarin alone (500 μM)] [Group IV]. Silymarin treatment maintains the integrity of erythrocytes by preventing hemolysis, protein thiol oxidation and by decreasing the activity of AChE. SEM observations indicate that B(a)P induced significant alteration in the morphology of erythrocytes to echinocytes, which may be due to the interaction of B(a)P with the membrane's outer phopholipid monolayer. The light microscopic and SEM images show that silymarin treatment maintains the normal discocytic morphology of erythrocytes. The protective effect of silymarin might be attributed to its chemical structure and membranotrophic nature. The components silibinin, silydianin, and silychristin have OH in the 3rd, 5th, and 7th carbon atoms that may account for its increased antioxidant activity and removal of ROS formed during B(a)P metabolism.

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Section: Discussionmentioning

confidence: 99%

Silymarin prevents the toxicity induced by benzo(a)pyrene in human erythrocytes by preserving its membrane integrity: Anin vitrostudy

2011

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“…Glutathione also acts as a substrate for the glutaredoxins, which reduce proteins that have been glutathionylated by reducing GSSG to a mixed disulphide protein and GSH. As the occurrence of mixed disulphides increases with increasing concentrations of GSSG, the ratio of the reduced to oxidized fractions (GSH/GSSG) of GSH within cells is often used as a reliable indicator of redox imbalance and has been shown to strongly influence cell cycle progression in proliferating cells (Menon et al, 2003 ; Öztürk and Gümüslü, 2004 ; Rose et al, 2012 ). The transcription factor nuclear factor-erythroid-2-related factor 2 (Nrf2) is essential for the coordinated induction of cytoprotective enzymes and related proteins in response to oxidative and electrophilic stresses (Itoh et al, 1999 ; Uruno and Motohashi, 2011 ).…”

Section: The Endogenous Antioxidant Systemmentioning

confidence: 99%

Activation of the hypothalamic-pituitary-adrenal stress axis induces cellular oxidative stress

et al. 2015

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Glucocorticoids released from the adrenal gland in response to stress-induced activation of the hypothalamic-pituitary-adrenal (HPA) axis induce activity in the cellular reduction-oxidation (redox) system. The redox system is a ubiquitous chemical mechanism allowing the transfer of electrons between donor/acceptors and target molecules during oxidative phosphorylation while simultaneously maintaining the overall cellular environment in a reduced state. The objective of this review is to present an overview of the current literature discussing the link between HPA axis-derived glucocorticoids and increased oxidative stress, particularly focussing on the redox changes observed in the hippocampus following glucocorticoid exposure.

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“…These results indicate that MDA levels shown to be higher after immobilization stress than before in mice which were given peppermint oil. Lipid peroxidation markers (MDA and TBARS) are known to be increased in stress conditions in plasma [36], erythrocyte [37-39] and different tissues [40-45] of animals. Our results in agreement with previous study [36].…”

Section: Discussionmentioning

confidence: 99%

Effect of Peppermint Oil on Serum Lipid Peroxidation and Hepatic Enzymes after Immobility Stress in Mice

Marjani

Rahmati²,

Mansourian³

et al. 2012

TOBIOCJ

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This study was undertaken to determine the influences of various doses of peppermint oil on the hepatic en-zymes, alanine transaminase, apartate tranaminase, alkaline phosphotase and gamma glutamyl transferase and the level of malondialdehyde in the serum of mice with and without immobility stress. The mice exposed to drink water, 0.9, 27 and 60 mg/kg peppermint oil from the days 1 to 5 for a period of 4 h before and after immobility stress. Serum MDA in-creased in treatment group II, III and IV after immobility stress. There was a significant decrease in ALT in treatment group III and IV after immobility stress. There were also significant decreases in ALP and GGT in treatment group IV af-ter immobility stress. This result may suggest that, MDA level is higher in immobilization stress group than in the un-immobilized animals in serum and this results show that enzyme activities decreased after immobilization stress.

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Age-related changes of antioxidant enzyme activities, glutathione status and lipid peroxidation in rat erythrocytes after heat stress

Cited by 47 publications

References 40 publications

Silymarin prevents the toxicity induced by benzo(a)pyrene in human erythrocytes by preserving its membrane integrity: Anin vitrostudy

Silymarin prevents the toxicity induced by benzo(a)pyrene in human erythrocytes by preserving its membrane integrity: Anin vitrostudy

Activation of the hypothalamic-pituitary-adrenal stress axis induces cellular oxidative stress

Effect of Peppermint Oil on Serum Lipid Peroxidation and Hepatic Enzymes after Immobility Stress in Mice

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