Age-related changes in turnover and concentration of a subset of thorax polypeptides from Drosophila melanogaster

Dubitsky, R.; Bensch, Klaus G.; Fleming, James E.

doi:10.1016/0047-6374(85)90088-0

Cited by 3 publications

References 21 publications

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Gene Expression and Protein Degradation

Remmen¹,

Ward²,

Sabia³

et al. 1995

Comprehensive Physiology

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Chromatin proteins and cellular aging The relationship between aging and protein synthesis RNA synthesis Protein synthesis and degradation during aging and senescence Aging and transcription Age-related changes in protein synthesis Enzymes, enzyme alteration, and protein turnover Age-related changes in the structure and function of chromatin Macromolecular methylation during aging The effect of age and nutrition on protein synthesis by cells and tissues from mammals Levels of specific messenger RNA species as a function of age Aging and gene expression Age-related changes of transcription and RNA processing Age-related changes in the synthesis of individual liver-specific proteins Effect of aging on translation and transcription Implication of protein oxidation in protein turnover, aging, and oxygen toxicity Biochemical markers of aging Protein oxidation and aging DNA methylation in aging and cancer RNA and protein metabolism in the aging brain Alterations in gene expression with aging Protein synthesis and the components of protein synthetic machinery during cellular ageing Effect of age on liver protein synthesis and degradation The next frontier: studies of the effects of age and diet on mammalian gene expression Protein synthesis, posttranslational modifications, and aging Gene expression and aging ~ TABLE 9.3. Effect of Age on the mRNA Levels in Liver

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Gene Expression and Protein Degradation

Remmen¹,

Ward²,

Sabia³

et al. 1995

Comprehensive Physiology

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Analysis of effect of age on synthesis of specific proteins by hepatocytes

Butler

Heydari

Richardson

1989

Journal Cellular Physiology

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The effect of age on the synthesis of specific proteins by hepatocytes was studied in Fischer F344 rats using two-dimensional polyacrylamide gel electrophoresis. Almost all proteins synthesized by hepatocytes from young rats were synthesized by hepatocytes isolated from old rats. Of over 500 proteins visually compared by two-dimensional polyacrylamide gel electrophoresis, only 11 proteins were observed to disappear and/or appear consistently with increasing age. The rates of synthesis of 36 randomly chosen proteins were quantified. Interestingly, the synthesis of 35 of the 36 proteins decreased between 5 and 30 months of age. The decrease in protein synthesis varied (15% to 70%) from one protein to another; i.e., a heterogeneity was observed in the age-related decrease in the synthesis of proteins. The age-related decrease in protein synthesis was statistically significant for 53% of the proteins studied. The total decrease in the rate of synthesis of all 36 proteins studied was 40% between 5 and 30 months of age, which is essentially the same as the decrease in total protein synthesis by suspension of hepatocytes isolated from 5- and 30-month-old rats. The results of this study demonstrate that the mechanism underlaying aging is different from development, which is characterized by a major change in the species of proteins synthesized by a cell.

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