Age-related Changes in the Ultrastructure and Membrane Properties of Brassica napus L. Seeds

Dawidowicz-Grzegorzewska, A.; Podstolski, Andrzej

doi:10.1093/oxfordjournals.aob.a088304

Cited by 16 publications

(9 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the sub-epidermal cells of the 3-year-old seed, the membrane of the protein bodies had ruptured, leading to the coalescence of protein bodies into a large confluent masse and the engulfment of lipid bodies by the protein substances. Similar observations have been reported by Smith (1978) and Dawidowicz-Grzegorzewska and Podstolski (1992) , in artificially aged lettuce and Brassica napus seeds. Protein bodies contain several hydrolytic enzymes, among them phosphatases.…”

Section: Discussionsupporting

confidence: 91%

“…As found for M. oleifera , lipid and protein bodies in cells of the embryo axis have been reported in the seed of many species such as B. napus ( Kuraś 1984 ; Dawidowicz-Grzegorzewska and Podstolski 1992 ), Origananum majorana ( Wiśniewska et al . 2006 ), Amaranthus hypocondriacus ( Ciombra and Salema 1994 ), Picea mariana ( Wang and Berjak 2000 ) and carrot ( Dawidowicz-Grzegorzewska 1997 ).…”

Section: Discussionsupporting

confidence: 55%

“…Protein bodies contain several hydrolytic enzymes, among them phosphatases. The release of their contents into the cytosol as a result of membrane deterioration can cause localized cellular autolysis ( Dawidowicz-Grzegorzewska and Podstolski 1992 ). The breakdown of the cell wall and the disintegration of the Golgi apparatus were also evident in severely deteriorated cells.…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Germination and ultrastructural studies of seeds produced by a fast-growing, drought-resistant tree: implications for its domestication and seed storage

2015

View full text Add to dashboard Cite

The inner layers of the seed coat that remain attached to the cotyledons probably play a role in seed dormancy of Moringa oleifera. Cotyledons of seeds stored for one year showed no sign of deterioration. In some cells of the three-year-old cotyledons, the membranes of the protein bodies were deteriorated. Cell deterioration was also marked by the collapse of the cell wall adjacent to the intercellular cavity. The decrease in seed viability during storage is associated with the loss in membrane integrity as confirmed by the increase in electrolyte leakage. The longevity of seeds can be extended if they are stored within their fruits under favourable conditions.

show abstract

Section: Discussionsupporting

confidence: 91%

Section: Discussionsupporting

confidence: 55%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Germination and ultrastructural studies of seeds produced by a fast-growing, drought-resistant tree: implications for its domestication and seed storage

2015

View full text Add to dashboard Cite

show abstract

“…It is essential for the rapid mobilisation of its storage lipid after germination that the oil bodies of the seed can withstand the rigours of dehydration and rehydration, while maintaining their structural integrity. Indeed, the non-viability of stored seeds has been correlated with a loss of oil body integrity, possibly due to the partial breakdown of their protective layer of oleosins [4,51].…”

Section: Discussionmentioning

confidence: 99%

Differential, temporal and spatial expression of genes involved in storage oil and oleosin accumulation in developing rapeseed embryos: implications for the role of oleosins and the mechanisms of oil-body formation

et al. 1993

View full text Add to dashboard Cite

The temporal and spatial expression of oleosin and delta 9-stearoyl-ACP desaturase genes and their products has been examined in developing embryos of rapeseed, Brassica napus L. var. Topas. Expression of oleosin and stearate desaturase genes was measured by in situ hybridisation at five different stages of development ranging from the torpedo stage to a mature-desiccating embryo. The temporal pattern of gene expression varied dramatically between the two classes of gene. Stearate desaturase gene expression was relatively high, even at the torpedo stage, whereas oleosin gene expression was barely detectable at this stage. By the stage of maximum embryo fresh weight, stearate desaturase gene expression had declined considerably while oleosin gene expression was at its height. In contrast to their differential temporal expression, the in situ labelling of both classes of embryo-specific gene showed similar, relatively uniform patterns of spatial expression throughout the embryo sections. Immunogold labelling of ultra-thin sections from radicle tissue with anti-oleosin antibodies showed similar patterns to sections from cotyledon tissue. However, whereas at least three oleosin isoforms were detectable on western blots of homogenates from cotyledons, only one isoform was found in radicles. This suggests that some of the oleosin isoforms may be expressed differentially in the various types of embryo tissue. The differential timing of stearate desaturase and oleosin gene expression was mirrored by similar differences in the timing of the accumulation of their ultimate products, i.e. storage oil and oleosin proteins.(ABSTRACT TRUNCATED AT 250 WORDS)

show abstract

“…What causes the degenerative events remains speculative. However, it is generally accepted that the decline in viability of naturally or artificially aged seeds results mainly from damage to nucleic acid and the deterioration of cellular membranes (Parrish and Leopold 1978;Ram and Wiesner 1988;Dawidowicz-Grzegorzewska and Podstolski 1992). The damage accumulates progressively both during normal seed storage and during accelerated ageing, and is affected by seed moisture content and temperature (Dong et al 1998;Yin et al 2000).…”

Section: Introductionmentioning

confidence: 99%

Effect of post-irradiation ageing on onion seeds

Amjad¹,

Anjum

2006

Acta Physiol Plant

View full text Add to dashboard Cite

Seeds of onion (Allium cepa) cv. Ailsa Craig were exposed to various doses of gamma radiation (0, 10, 20, 40, 80 and 100 krad) and subjected to accelerated ageing (RH 100%, 42°C) for 12 h. Radiation doses significantly affected the percentage of normal seedlings, abnormality types (%) and seedling growth. Seed viability, conductivities of seed leachates, final germination (%), germination speed and time to reach 50% germination (T 50 ) were not affected by the seed irradiation. Accelerated ageing after irradiation had significant influence on seed viability, conductivities of seed leachates, final germination (%) and percentage of normal seedlings. Germination speed, T 50 and seedling growth were not affected by the accelerated ageing. However, all the parameters studied were significantly influenced by the interaction of radiation doses and accelerated ageing. Accelerated ageing generally enhanced the damaging effects of irradiation on seeds. Therefore, it was concluded that onion seeds should not be exposed to adverse environmental conditions after irradiation.

show abstract

Age-related Changes in the Ultrastructure and Membrane Properties of Brassica napus L. Seeds

Cited by 16 publications

References 0 publications

Germination and ultrastructural studies of seeds produced by a fast-growing, drought-resistant tree: implications for its domestication and seed storage

Germination and ultrastructural studies of seeds produced by a fast-growing, drought-resistant tree: implications for its domestication and seed storage

Differential, temporal and spatial expression of genes involved in storage oil and oleosin accumulation in developing rapeseed embryos: implications for the role of oleosins and the mechanisms of oil-body formation

Effect of post-irradiation ageing on onion seeds

Contact Info

Product

Resources

About