Age-related changes in the composition of proteins in the trabecular meshwork of the human eye

Horstmann, Hans‐Joachim; Rohen, Johannes W.; Sames, K.

doi:10.1016/0047-6374(83)90069-6

Cited by 41 publications

(11 citation statements)

References 20 publications

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“…Indeed, dysfunction of the TM increases the resistance of the outflow pathway and induces an increase in intraocular pressure. The number of TM cells decreases with aging [15] the histopathologic findings of primary open-angle glaucoma tissue being similar to those of aged tissue [16], [17]. Reactive oxygen species (ROS) damage TM cells, induce apoptosis, and promote cellular aging in this tissue [18], [19].…”

Section: Introductionmentioning

confidence: 99%

Oxidative Damage and Autophagy in the Human Trabecular Meshwork as Related with Ageing

et al. 2014

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Autophagy is an intracellular lysosomal degradation process induced under stress conditions. Autophagy also plays a major role in ocular patho-physiology. Molecular aging does occur in the trabecular meshwork, the main regulator of aqueous humor outflow, and trabecular meshwork senescence is accompanied by increased oxidative stress. However, the role of autophagy in trabecular meshwork patho-physiology has not yet been examined in vivo in human ocular tissues. The purpose of the herein presented study is to evaluate autophagy occurrence in ex-vivo collected human trabecular meshwork specimens and to evaluate the relationship between autophagy, oxidative stress, and aging in this tissue. Fresh trabecular meshwork specimens were collected from 28 healthy corneal donors devoid of ocular pathologies and oxidative DNA damage, and LC3 and p62 protein expression analyzed. In a subset of 10 subjects, further to trabecular meshwork proteins, the amounts of cathepesin L and ubiquitin was analyzed by antibody microarray in aqueous humor. Obtained results demonstrate that autophagy activation, measured by LC3II/I ratio, is related with. oxidative damage occurrence during aging in human trabecular meshwork. The expression of autophagy marker p62 was lower in subjects older than 60 years as compared to younger subjects. These findings reflect the occurrence of an agedependent increase in the autophagy as occurring in the trabecular meshwork. Furthermore, we showed that aging promotes trabecular-meshwork senescence due to increased oxidative stress paralleled by autophagy increase. Indeed, both oxidative DNA damage and autophagy were more abundant in subjects older than 60 years. These findings shed new light on the role of oxidative damage and autophagy during trabecular-meshwork aging.

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Section: Introductionmentioning

confidence: 99%

Oxidative Damage and Autophagy in the Human Trabecular Meshwork as Related with Ageing

et al. 2014

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“…It is important to maintain the normal physiological function of the trabecular meshwork because it functions in regulating outflow [20]. The number of trabecular meshwork cells decreases with aging [45] and the histopathologic findings of primary open-angle glaucoma tissue are similar to those of aged cells [46-49]. …”

Section: Discussionmentioning

confidence: 99%

Effect of Advanced Glycation End Products on Oxidative Stress and Senescence of Trabecular Meshwork Cells

Park

Kim

2012

Korean J Ophthalmol

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PurposeTo investigate the effect of advanced glycation end products (AGE) on oxidative stress and cellular senescence in cultured human trabecular meshwork cells (HTMC).MethodsPrimarily cultured HTMC were exposed to 0, 10, 50, 100, 200 µg/mL of glycated bovine serum albumin (G-BSA) for 5 days. Also co-exposed were L-arginine, sepiapterin, and antioxidant N-acetylcysteine (NAC). Cellular survival and production of nitric oxide (NO), superoxide, and reactive oxygen species were assessed by 3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide assay, Griess assay, cytochrome c assay, and dichlorofluorescin diacetate assay, respectively. Senescence-associated β-galactosidase staining was performed to quantify the degree of cellular senescence.ResultsG-BSA decreased cellular survival, NO production, and increased superoxide production significantly in a dose-dependent manner. The effects of G-BSA were abolished with co-exposure of L-arginine, sepiapterin, and NAC. G-BSA enhanced cellular senescence accompanied by increased production of reactive oxygen species. G-BSA-induced cellular senescence was suppressed by application of L-arginine, sepiapterin, and NAC.ConclusionsAGE enhances cellular senescence of HTMC accompanied with increased oxidative stress. AGE-induced oxidative stress and cellular senescence could be delayed by application of anti-oxidants.

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“…Some authors have reported success with trabeculotomy in adults [55], while others have reported poor success and suggested that inadvertent filtration blebs may explain the successful cases [56]. Higher success rates for angle surgery in infants may be due to the higher elasticity of the trabecular tissues in infants [57], which allows the cut ends of the TM to retract, preventing them from re-approximating and closing from fibrosis. The thicker and less elastic tissue of adults may predispose the ends of the TM to fold back into their original position and fibrose together after goniotomy or trabeculotomy [51,58].…”

Section: Angle Surgerymentioning

confidence: 96%

Advances in glaucoma surgery

Filho

Sit

2009

Expert Review of Ophthalmology

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Despite being introduced over 50 years ago, the current gold standard for glaucoma surgery is still trabeculectomy. However, significant complications can occur with the procedure and, consequently, recent developments in glaucoma surgery have focused on improving safety and reliability. These developments can be categorized into three main areas: advances in filtering surgery, angle surgery and uveoscleral shunts. Each of these approaches has advantages and disadvantages, which are discussed in this review. In general, angle surgery and uveoscleral shunts do not appear to offer as low an intraocular pressure as filtering surgery, but appear to have fewer serious complications than filtering surgery. However, clinical data for these newer approaches are still limited in many cases, and further research is required to determine the role of new surgical options in glaucoma management.

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Age-related changes in the composition of proteins in the trabecular meshwork of the human eye

Cited by 41 publications

References 20 publications

Oxidative Damage and Autophagy in the Human Trabecular Meshwork as Related with Ageing

Oxidative Damage and Autophagy in the Human Trabecular Meshwork as Related with Ageing

Effect of Advanced Glycation End Products on Oxidative Stress and Senescence of Trabecular Meshwork Cells

Advances in glaucoma surgery

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