Age related changes in the elastic fiber system of the interfoveolar ligament

Quintas, Mario Luiz; Rodrigues, Consuelo Junqueira; Jin, Hwan Yoo; Rodrigues, Aldo Junqueira

doi:10.1590/s0041-87812000000300003

Cited by 27 publications

(27 citation statements)

References 12 publications

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“…In the present work, the linear density of elaunin fibers and elastic fibers was increased in the elderly group compared to the young group, and oxytalan fibers was decreased in the elderly group compared to the young group. Similar results were described by [44,45] , who found that during aging, there was a decrease in oxytalan fibers and an increase mature elaunin and elastic fibers. Furthermore, elastin was thicker and more fragmented in older people, and there was a greater deposition of calcium in the amorphous material.…”

Section: Discussionsupporting

confidence: 89%

“…Moreover, study of the interspinous ligament during aging found the disappearance of oxytalan fibers [48] , which is in agreement with our results. In addition to our study, authors suggested that aging is accompanied by a significant and progressive reduction in oxytalan fibers and significant increases in mature elaunin and elastic fibers in the interfoveolar ligament [44] . Elaunin fibers play an intermediary role between oxytalan fibers and mature elastic fibers, providing functional adaptation in different tissues.…”

Section: Discussionsupporting

confidence: 79%

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Effects of aging on the architecture of the ileocecal junction in rats

Brito

Chopard

Cury

et al. 2016

WJGPT

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AIM:To evaluate the structural organization of the elastic and collagen fibers in the region of the ileocecal transition in 30 young and old male Wistar rats. METHODS:Histology, immunohistochemistry (IHC), transmission electron microscopy and scanning electron microscopy were employed in this study. The results demonstrated that there was a demarcation of the ileocecal region between the ileum and the cecum in both groups. RESULTS:The connective tissue fibers had different distribution patterns in the two groups. IHC revealed the presence of nitric oxide synthase, enteric neurons and smooth muscle fibers in the ileocecal junctions (ICJs) of both groups. Compared to the young group, the elderly group exhibited an increase in collagen type Ⅰ fibers, a decrease in collagen type Ⅲ fibers, a decreased linear density of oxytalan elastic fibers, and a greater linear density of elaunin and mature elastic fibers. CONCLUSION:The results revealed changes in the patterns of distribution of collagen and elastic fibers that may lead to a possible decrease in ICJ functionality. Core tip: The ileocolonic sphincter controls the forward and backward flow by integrating its motility with that of the distal ileum and proximal the ileocolonic sphincter controls the forward and backward flow by integrating its motility with that of the distal ileum and proxima. The ileocecal junction (ICJ) includes the muscle bundles in the terminal ileum, the intrinsic nerve plexus. The ICJ includes the muscle bundles in the terminal ileum, the intrinsic nerve plexus. Given the importance of knowing how the ICJ changes with age, the objective of this study was to characterize the morphological changes in the ICJ in rats aged 21 d and 2 years, using optical microscopy and electronic scanning and transmission methodologies. Additionally, the neurochemical characterization of the inhibitory neurons of the myenteric plexus, which are immunoreactive to the enzyme nitric oxide synthase, and the staining of the neuronal population were employed to identify immunoreactivity to HuC/D.de Brito MC, Chopard RP, Cury DP, Watanabe IS, Mendes CE, Castelucci P. Effects of aging on the architecture of the ileocecal junction in rats.

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Section: Discussionsupporting

confidence: 89%

Section: Discussionsupporting

confidence: 79%

Effects of aging on the architecture of the ileocecal junction in rats

Brito

Chopard

Cury

et al. 2016

WJGPT

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“…elastic fibers and reduction in the amount of oxytalan fibers. These structural changes can explain the high incidence of groin hernias in patients after the fifth decade (Rodrigues Jr et al 6 , Quintas et al 7 ). With aging there was a decreased collagen content in the fascias, as well as decreased extractability of collagen with pepsin (Pans et al 17 ).…”

Section: Discussionmentioning

confidence: 99%

“…Rodrigues Junior et al 6 and Quintas et al 7 have shown structural and quanti-tative age-related changes in the TF elastic fibers. These results indicate clearly that there is a morphological correlate to the loss of tensile resistance and elasticity of the TF with aging, and this loss can explain the high incidence of inguinal hernia in patients after the fifth decade of life.…”

Section: Introductionmentioning

confidence: 98%

Quantitative analysis of collagen and elastic fibers in the transversalis fascia in direct and indirect inguinal hernia

Rodrigues

Cunha

et al. 2002

Rev. Hosp. Clin.

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PURPOSE:Our previous studies demonstrated structural and quantitative age-related changes of the elastic fibers in transversalis fascia, which may play a role in inguinal hernia formation. To verify whether there were differences in the extracellular matrix between direct and indirect inguinal hernia, we studied the amount of collagen and elastic fibers in the transversalis fascia of 36 male patients with indirect inguinal hernia and 21 with direct inguinal hernia. MATERIAL AND METHODS:Transversalis fascia fragments were obtained during surgical intervention and underwent histological quantitative analysis of collagen by colorimetry and analysis of elastic fibers by histomorphometry. RESULTS:We demonstrated significantly lower amounts of collagen and higher amounts of elastic fibers in transversalis fascia from patients with direct inguinal hernia compared to indirect inguinal hernia patients. The transversalis fascia from direct inguinal hernia patients showed structural changes of the mature and elaunin elastic fibers, which are responsible for elasticity, and lower density of oxytalan elastic fibers, which are responsible for resistance. These changes promoted loss of resiliency of the transversalis fascia.CONCLUSION: These results improve our understanding of the participation of the extracellular matrix in the genesis of direct inguinal hernia, suggesting a relationship with genetic defects of the elastic fiber and collagen synthesis. DESCRIPTORS: Inguinal hernia. Transversalis fascia. Elastin. Collagen. Extracellular matrix.

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“…Research by our group has shown age-related structural changes (breaching and wrapping) in the elastic fibers, with ultrastructural studies showing a large decrease in the quantity of microfibril component ( Rodrigues Jr. et al., 1990;Quintas et al, 2000). Some studies have shown reduced amounts of collagen in the fascia transversalis of patients with direct inguinal hernia compared to those with indirect hernia, suggesting that the development of some hernias might be influenced by disturbances in collagen metabolism (Pans et al, 2001;Rodrigues Jr et al, 2002).…”

Section: Introductionmentioning

confidence: 93%

Elastin (ELN) gene point mutation in patients with inguinal hernia

2006

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Groin hernias emerge at the myopectineal orifice of Fruchaud which is closed off by the fascia transversalis. Our previous studies showed structural and quantitative changes of the fascia transversalis elastic fibers of inguinal hernia patients and elderly people. The present study used single-strand conformation polymorphism (SSCP) elastin (analysis to investigate the 34 exons of the ELN gene of 49 inguinal hernia patients (7 females, 42 males aged 58.7 ± 19.82 years) and 75 non-herniated controls (35 females, 40 males aged 46.2 ± 14.32 years). We found that 47 patients and 24 controls had an abnormal exon 20 pattern caused by a g28197A > G missense mutation leading to an S422G amino acid substitution in the elastin hydrophobic domain. The g28197A > G allele frequency was 0.71 ± 0.045 in hernia patients and 0.21 ± 0.030 in controls and 23 patients and 7 controls were g28197A > G homozygous and 24 patients and 17 controls were heterozygous. This point-mutation showed a statistically significant association with inguinal hernia, chi-squared being 46.89 (p < 0.001) and the odds ratio 49.93 (95% confidence interval of @11 to 223). These results indicate that the g28197A > G mutation is involved in the genesis of inguinal hernia (possibly due to abnormal elastic fiber production) and explains impaired fascia transversalis function. Key words: fascia transversalis, extracellular matrix, elastic fibers, elastin gene, inguinal hernia. All groin hernias emerge at the myopectineal orifice of Fruchaud which is closed off by the fascia transversalis, a connective tissue composed of a framework of elastic and collagen fibers, which supports the abdominal tension forces.The elastic fibers are composed of microfibrils and elastin and are responsible for the resilience of the fascia transversalis. Research by our group has shown age-related structural changes (breaching and wrapping) in the elastic fibers, with ultrastructural studies showing a large decrease in the quantity of microfibril component ( Rodrigues Jr. et al., 1990;Quintas et al., 2000). Some studies have shown reduced amounts of collagen in the fascia transversalis of patients with direct inguinal hernia compared to those with indirect hernia, suggesting that the development of some hernias might be influenced by disturbances in collagen metabolism (Pans et al., 2001;Rodrigues Jr et al., 2002). Taken together, these results indicate that there is a loss of tensile resistance and elasticity in the fascia transversalis tissue which may explain the high incidence of inguinal hernia after the fifth decade of life.Mutations in the elastin (ELN) gene may be responsible for disorders of the elastic fiber system because they have been observed in cases of supravalvular aortic stenosis and autosomal dominant cutis laxa, individuals with either of these conditions may show smooth and soft skin, hernias, gastrointestinal diverticula and genital prolapse (Ewart et al., 1994;Li et al., 1997;Meng et al., 1998;Tassabehji et al., 1998). These reports and our previous st...

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Age related changes in the elastic fiber system of the interfoveolar ligament

Cited by 27 publications

References 12 publications

Effects of aging on the architecture of the ileocecal junction in rats

Effects of aging on the architecture of the ileocecal junction in rats

Quantitative analysis of collagen and elastic fibers in the transversalis fascia in direct and indirect inguinal hernia

Elastin (ELN) gene point mutation in patients with inguinal hernia

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