Age-related changes in substance P-immunoreactive nerve structures of the rat recto-anal region

Lazarov, Nikolai; Itzev, Dimitar E.; Usunoff, Kamen G.; Negrev, Negrin; Radomirov, R.

doi:10.2478/s11536-009-0098-2

Cited by 3 publications

(5 citation statements)

References 25 publications

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“…Similar to our findings, an absence of significant SP‐immunostaining in the myenteric neuronal bodies has previously been reported (Lazarov et al, 2009). Conversely, another study has shown an intense staining of myenteric neuronal somata (Hens et al, 2002).…”

Section: Discussionsupporting

confidence: 93%

“…In this regard, the present findings strongly reinforce the suggestion that SP-positive structures are an important component of the enteric circuitry. Similar to our findings, an absence of significant SPimmunostaining in the myenteric neuronal bodies has previously been reported (Lazarov et al, 2009). Conversely, another study has shown an intense staining of myenteric neuronal somata (Hens et al, 2002).…”

Section: Discussionsupporting

confidence: 93%

“…This is also true for studies in dogs showing that such beaded SP-immunopositive fibers synapse on the surface of SP-immunonegative myenteric neurons (Polidoro et al, 2017). The presence of SPimmunoreactive fibers in the circular muscle layer is in accordance with previous experiments, conducted on the rat small and large intestine (Lazarov et al, 2009;Mitsui, 2011). The observations related to the longitudinal muscle layer, however, are contradictory, since according to Bauer (1993) in the guinea pigs and rats SPergic fibers are not only a common finding, but are even shown to have a quite significant excitatory motor influence.…”

Section: Discussionsupporting

confidence: 88%

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Neurochemical profile of the myenteric plexus in the rat colorectal region

Kirov

Atanasova

Lazarov

2022

The Anatomical Record

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The enteric nervous system, a major subdivision of the autonomic nervous system, is known for its neurochemical heterogeneity and complexity. The myenteric plexus, one of its two principal components, primarily controls peristalsis and its dysfunction may lead to a number of gastrointestinal motility disorders. The myenteric neurons have been described to use a wide variety of neurotransmitters although no evidence has been reported for the existence of adrenergic neurons in the hindgut. This study aims at elucidating the chemical coding of neurons in the myenteric plexus of the rat colon and anorectal region with particular emphasis on cholinergic and the so‐called nonadrenergic, noncholinergic (NANC) transmitter systems. The immunostaining for choline acetyltransferase revealed an intense staining of the myenteric ganglia with clear delineation of their neuronal cell bodies and without local distributional differences in the colonic region. The myenteric ATPergic structures were mostly limited to fiber bundles surrounding unstained myenteric neurons and penetrating the two muscle layers. We also observed an abundance of intensely stained varicose substance P‐immunopositive fibers, ensheathing the immunonegative myenteric neuronal cell bodies in a basket‐like manner. Applying NADPH‐diaphorase histochemistry and nitric oxide synthase immunohistochemistry, we were able to demonstrate numerous nitrergic somata of myenteric neurons with Dogiel Type I morphology. Apart from the observed nitrergic distributional patterns, no distinct variations were found in the staining intensity or distribution of myenteric structures in the colon and anorectal area. Our results suggest that myenteric neurons in the distal intestinal portion utilize a broad spectrum of enteric transmitters, including classical and NANC transmitters.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionsupporting

confidence: 93%

Section: Discussionsupporting

confidence: 88%

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Neurochemical profile of the myenteric plexus in the rat colorectal region

Kirov

Atanasova

Lazarov

2022

The Anatomical Record

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“…2B). Such negative perikaryal staining has been described in previous studies (27,28). On the other hand, positive staining has been observed in other experiments (29,30).…”

Section: Peptidergic Transmission In the Myenteric Plexussupporting

confidence: 79%

Morphology of the myenteric plexus and nanc transmission in therat colorectal region

Kirov,

Malinova,

Atanasova

et al. 2023

Biomedical Reviews

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The enteric nervous system exerts local control on the gastrointestinal motility, exocrine and endocrine secretions, ensures proper blood supply, and modulates the immune and inflammatory processes in the gut. The aim of this paper is to give insights into current advances and present knowledge about morphology of the myenteric plexus and the neurochemical coding of adult rat myenteric neurons in the large intestine with a special emphasis on non-adrenergic, non-cholinergic (NANC) transmitters. Major neuroactive substances involved in the NANC transmission include nitric oxide (NO), tachykinins, mostly substance P (SP), and adenosine triphosphate (ATP), co-localized in variable proportions with other neuroactive substances. After applying NADPH-diaphorase enzyme histochemistry and neuronal NO synthase (nNOS) immunohistochemistry, we were able to demonstrate numerous nitrergic somata of myenteric neurons with Dogiel type I morphology. In addition to the observed nitrergic distributional patterns, significant variations were registered in the staining intensity of myenteric structures in the colon and anorectal area. Immunohistochemistry for SP revealed an abundance of intensely stained SP-immunoreactive varicose fibers, ensheathing the immunonegative myenteric neuronal cell bodies in a basket-like manner. ATPergic structures were mostly limited to fiber bundles surrounding unstained myenteric neurons and penetrating the two muscle layers. An important finding was the notable presence of purinergic immunoreactive nerve fiber bundles in the longitudinal muscle layer. In conclusion, our results show that the cytoarchitecture and morphometric properties appeared to mimic their equivalent in the more proximal parts of the rat digestive tract and in other experimental animals.

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“…At the time of sacrifice, all the animals were anesthetized with Thiopental (50 mg/kg) intraperitoneally, and the animals were transcardially perfused, first, with warm heparinized 0.05 mol/l PBS (pH 7.4), followed by freshly prepared 4% paraformaldehyde in 0.1 mol/l phosphate buffer [18]. The stomachs were dissected and rinsed with PBS (pH 7.4), poured into the gastric cavity with 40 g/l paraformaldehyde (until three to four times of normal stomach volume), and placed in the same fixative for 4-6 h (4ºC).…”

Section: Animal Usedmentioning

confidence: 99%