Age-related changes in lipid and carbohydrate metabolism of the genetically obese mouse

Menahan, Lawrence A.

doi:10.1016/0026-0495(83)90225-1

Cited by 39 publications

(36 citation statements)

References 29 publications

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“…It has been reported that ob/ob mice displayed higher levels of intracellular hepatic triglycerides as early as 2 weeks after birth [27,28]. The level of fractional lipid mass (fLM) in ob/ob mice found in this study is in line with results obtained using conventional techniques [28].…”

Section: H Mr Spectroscopic Information On Hepatic Lipid Composition supporting

confidence: 81%

“…fLM values in ob/? controls were slightly higher than literature values though the difference was not significant (3.4 ± 1.0% vs. 2.7% estimated from literature [28] assuming a mean molecular weight of triglycerides of 800 Dalton). An important finding was a significantly longer mean chain length in ob/ob as compared to ob/?…”

Section: H Mr Spectroscopic Information On Hepatic Lipid Composition mentioning

confidence: 42%

“…In ob/ob mice, influx of free fatty acid from circulation is increased [39]. Moreover, fatty acid synthesis activity, de novo hepatic long chain fatty acid and triglyceride synthesis are elevated, however the very low density lipoprotein synthesis and TG export are not increased [3,28,40]. In combination with impaired liver mitochondrial b-oxidation, hepatic lipids accumulate, in particular the long chain fatty acyl-CoA [27,31].…”

Section: H Mr Spectroscopic Information On Hepatic Lipid Composition mentioning

confidence: 99%

“…They are characterized by leptin deficiency, which leads to increased food intake and reduced energy expenditure; in addition, ob/ob mice display a slightly increased feed efficiency (increased body weight gain for identical caloric intake as compared to ob/? controls) [3,22,27,28]. This metabolic condition causes a redistribution of fat storage from fat pads to non-adipose tissue such as liver, skeletal muscle, heart and pancreas.…”

Section: Introductionmentioning

confidence: 99%

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Hepatic lipid composition differs between ob/ob and ob/+ control mice as determined by using in vivo localized proton magnetic resonance spectroscopy

Danzer

Fuchs

et al. 2012

Magn Reson Mater Phy

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Object Hepatic lipid accumulation is associated with nonalcoholic fatty liver disease, and the metabolic syndrome constitutes an increasing medical problem. In vivo proton magnetic resonance spectroscopy ( 1 H MRS) allows the assessment of hepatic lipid levels noninvasively and also yields information on the fat composition due to its high spectral resolution. Materials and methods We applied 1 H MRS at 9.4T to study lipid content and composition in eight leptin-deficient ob/ob mice as a model of obesity and in four lean ob/? control mice at 24 weeks of age. PRESS sequence was used. For accurate estimation of signal intensity, differences in relaxation behavior of individual signals were accounted for each mouse individually. Also, in order to minimize spectral degrading due to motion artifacts, respiration gating was applied.Results Significant differences between ob/ob and ob/? control mice were found in both lipid content and composition. The mean chain length was found to be significantly longer in ob/ob mice with a higher fraction of monounsaturated lipids. Conclusion 1 H MRS enables accurate assessment in hepatic lipids in mice, which is attractive for mechanistic studies of altered metabolism given the large number of genetically engineered mouse models available.

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Section: H Mr Spectroscopic Information On Hepatic Lipid Composition supporting

confidence: 81%

Section: H Mr Spectroscopic Information On Hepatic Lipid Composition mentioning

confidence: 42%

Section: H Mr Spectroscopic Information On Hepatic Lipid Composition mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Hepatic lipid composition differs between ob/ob and ob/+ control mice as determined by using in vivo localized proton magnetic resonance spectroscopy

Danzer

Fuchs

et al. 2012

Magn Reson Mater Phy

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“…The ob/ob mouse develops mild type 2 diabetes by 8-9 weeks of age, but then demonstrates a phenotypic improvement by 4-5 months of age (Garthwaite et al 1980, Menahan 1983, Tassava et al 1992. Leptin induces UCP2 expression in islets ), yet in the ob/ob mouse the upregulation of UCP2 (Zhang et al 2001) is clearly independent of leptin.…”

Section: Introductionmentioning

confidence: 99%

Endogenous islet uncoupling protein-2 expression and loss of glucose homeostasis in ob/ob mice

Saleh¹,

Wheeler²,

Chan³

2006

Journal of Endocrinology

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We hypothesized that the loss of glucose homeostasis in ob/ob mice is associated with upregulation of islet uncoupling protein-2 (UCP2) expression, leading to impaired glucosestimulated insulin secretion (GSIS). Changes in glucose homeostasis in lean and ob/ob mice from 5 to 16 weeks were assessed by fasting blood glucose, plasma insulin, oral glucose tolerance, and tissue insulin sensitivity. In vitro GSIS and ATP content were assayed in isolated islets, while UCP2 expression was determined by quantitative real-time PCR and immunoblotting. Short-term reduction of UCP2 expression was achieved through transfection of islets with specific small interfering RNA. Insulin resistance was detected in 5-week-old ob/ob mice, but GSIS and blood glucose levels remained normal. By 8 weeks of age, ob/ob mice displayed fasting hyperglycemia, hyperinsulinemia and glucose intolerance, and also had elevated non-esterified fatty acid concentration in plasma. In vitro, GSIS and ATP generation were impaired in ob/ob islets. Islet UCP2 expression was elevated at 5 and 8 weeks of age. Short-term knockdown of islet UCP2 increased GSIS in islets of lean mice, but had no effect in islets from ob/ob mice. Loss of glucose homeostasis and impairment of insulin secretion from isolated islets at 8 weeks in ob/ob mice is preceded by an increase in UCP2 expression in islets. Moreover, the glucolipotoxic conditions observed are predicted to increase UCP2 activity, contributing to lower islet ATP and GSIS.

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New and Efficient Routes to Biomolecules Substituted with Cyclopentadienyltricarbonylrhenium and -Technetium Derivatives

et al. 2001

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The small, compact, robust, and nonpolar units of [CpM(CO)3] (M = Re, Tc) coupled with biomolecules may be considered as bioorganometallic entities of potential interest in the field of medicinal chemistry. However, the short half-life of useful radionuclides (186Re t1/2 = 3.7 d, 188Re t1/2 = 16.8 h, 99mTc t1/2 = 6 h), the risks inherent in their use, and their cost have led chemists to search for novel synthetic strategies that allow the rapid introduction of the [CpM(CO)3] moiety as a late step in the course of synthesizing the target molecule. The present paper describes different strategies recently reported in the literature to tackle this problem.

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Age-related changes in lipid and carbohydrate metabolism of the genetically obese mouse

Cited by 39 publications

References 29 publications

Hepatic lipid composition differs between ob/ob and ob/+ control mice as determined by using in vivo localized proton magnetic resonance spectroscopy

Hepatic lipid composition differs between ob/ob and ob/+ control mice as determined by using in vivo localized proton magnetic resonance spectroscopy

Endogenous islet uncoupling protein-2 expression and loss of glucose homeostasis in ob/ob mice

New and Efficient Routes to Biomolecules Substituted with Cyclopentadienyltricarbonylrhenium and -Technetium Derivatives

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