Age-related changes in cerebral congenital toxoplasmosis: Histopathological and immunohistochemical evaluation

Saad, Abeer E.; Ashour, Dalia S.; Dawood, Lamees M.; El-Shorbagy, Safinaz H.

doi:10.1016/j.jneuroim.2020.577384

Cited by 6 publications

(2 citation statements)

References 71 publications

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“…During pregnancy, T. gondii can infect the fetus via placenta causing abortion or congenital abnormalities (Arefkhah et al, 2019), with affection of about 190,000 individuals worldwide annually (Saad et al, 2020). Pathogenesis is related to induction of oxidative stress and lipid peroxidation that cause overproduction of Reactive Oxygen free Radicals (ROR) with reduction of endogenous antioxidants (Motavalli et al, 2018) that makes Malondialdehyde (MDA); a biomarker of lipid peroxidation and oxida-tive stress, increases in chronic acquired toxoplasmosis (Al-Kuraishy et al, 2019) and becomes a good indicative method for toxoplasmosis and assessment of its treatment (Kiran et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Efficacy of Murine Platelet Rich-Plasma Versus Spiramycin in Treatment of Chronic Toxoplasmosis Infected Mice

Ibrahim

MOHAMED

FOAAD

et al. 2022

Journal of the Egyptian Society of Parasitology

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Apicomplexan parasite, Toxoplasma gondii, is an obligate intracellular parasite, infecting and replicating within nucleated cells in many homoeothermic organisms, mammals and birds with the possibility of causing serious health problems. Recent studies indicated several side effects to the current drugs like Pyrimethamine ® & Sulfadiazine ® . This work evaluated murine platelet rich plasma (PRP) efficacy versus Spiramycin ® treating toxoplasmosis infected mice parasitological, biochemical and histopathological studies. Fifty five immunocompetent male mice were divided into 5 groups of 11 mice each. G1: non-infected control, G2: infected non-treated control, G3: infected and treated with spiramycin, G4: infected and treated with murine PRP, and G5: infected and treated with combined spiramycin and PRP.Spiramycin and PRP treated mice gave the highest reduction in Toxoplasma tissue cysts number, lowest level of serum malondialdehyde (MDA) and marked pathological improvement.

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Section: Introductionmentioning

confidence: 99%

Efficacy of Murine Platelet Rich-Plasma Versus Spiramycin in Treatment of Chronic Toxoplasmosis Infected Mice

Ibrahim

MOHAMED

FOAAD

et al. 2022

Journal of the Egyptian Society of Parasitology

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“…About one-third of the world's population are reportedly infected [2] . Congenital toxoplasmosis is estimated to impact 190,000 individuals worldwide per year, resulting in a significant global burden of disease of 1.20 million disability-adjusted life years [3] . The ingestion of undercooked and raw meat containing bradyzoite cysts has been linked to human infections.…”

Section: Introductionmentioning

confidence: 99%

Behavior and neuropsychiatric changes in experimental chronic toxoplasmosis: Histopathological and immunohistochemical studies

Etewa

Sarhan

Moawad

et al. 2021

Parasitologists United Journal

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Background: T. gondii is an intracellular protozoan parasite that can establish a latent infection in the central nervous system that may develop into chronic inflammation resulting in neurobehavioral problems in the host. The processes behind these alterations are still largely mysterious. Objective: Detection of behavioral, histopathological and immunohistochemical changes in mice infected by T. gondii Me49 strain. Material and Methods: A total of 105 adult male Swiss albino mice were divided into 60 used for experimental infection, and 45 as control. Assessment of physical appearance was monitored for acute toxoplasmosis daily for three weeks post infection (PI). Correlation between behavior changes and the degree of infection was conducted by measuring histopathological (H&E and silver stain) and immunohistochemical (presence or absence of CD3, CD138 and caspase-3 immunoreactive cells) parameters weekly starting from 7 th week to the 12 th week PI. Results: Infected mice had neurobehavioral problems. Variable degrees of perivascular and interstitial inflammatory infiltrates, astrocytosis, deteriorated neurons, and meningitis were demonstrated by histopathology when compared to uninfected controls. Inflammatory cells (mainly lymphocytes) entered the parenchyma at mild, moderate, and severe levels in the brains of infected mice. Immunohistochemical assessment of CD3, CD138 and caspase-3 revealed a substantial increase in CD3 expression by clusters of activated astrocytes in the cerebral parenchyma, suggesting an increase in astrocyte numbers and function that was progressive over time. CD138 and caspase-3 immunoreactivity showed decreased expression by the activated astrocytes. Conclusion: Chronic toxoplasmosis causes deterioration in cognitive and emotional behavior of the infected host, resulting in neuropsychiatric and behavioral disturbances.

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Type-1 diabetes mellitus down-regulated local cerebral glial fibrillary acidic protein expression in experimental toxoplasmosis

et al. 2023

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Age-related changes in cerebral congenital toxoplasmosis: Histopathological and immunohistochemical evaluation

Cited by 6 publications

References 71 publications

Efficacy of Murine Platelet Rich-Plasma Versus Spiramycin in Treatment of Chronic Toxoplasmosis Infected Mice

Efficacy of Murine Platelet Rich-Plasma Versus Spiramycin in Treatment of Chronic Toxoplasmosis Infected Mice

Behavior and neuropsychiatric changes in experimental chronic toxoplasmosis: Histopathological and immunohistochemical studies

Type-1 diabetes mellitus down-regulated local cerebral glial fibrillary acidic protein expression in experimental toxoplasmosis

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