Age-related changes in an antioxidant defense system in elderly patients with essential hypertension compared with healthy controls

Rybka, Joanna; Kupczyk, Daria; Kędziora–Kornatowska, Kornelia; Pawluk, Hanna; Czuczejko, Jolanta; Szewczyk-Golec, Karolina; Kozakiewicz, Mariusz; Antonioli, Marco; Carvalho, Lívia A.; Kędziora, J

doi:10.1179/174329211x13002357050897

Cited by 50 publications

(27 citation statements)

References 47 publications

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“…The percentage of smokers was also higher in the MDD group than in the healthy group. Since there is evidence that increased age and smoking could contribute to oxidative stress [43,44,45], in further statistical analyses, we also examined if the effects of age and smoking contributed to observed differences between MDD and control groups. …”

Section: Resultsmentioning

confidence: 99%

Lymphocyte Levels of Redox-Sensitive Transcription Factors and Antioxidative Enzymes as Indicators of Pro-Oxidative State in Depressive Patients

Lukić¹,

Mitić²,

Djordjević³

et al. 2014

Neuropsychobiology

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Background: Oxidative stress is reliably observed in major depressive disorder (MDD). However, molecular data on the principal cellular redox-sensitive transcriptional factors and the levels of their downstream-regulated antioxidant enzymes in MDD are scarce. Methods: In the peripheral blood mononuclear cells (PBMC) of subjects with a current episode of MDD (n = 30) and healthy controls (n = 35), we investigated alterations in the levels of redox-sensing nuclear factor (erythroid-derived 2)-like 2 (Nrf2) protein, its inhibitor Keap1, and nuclear factor-κB (NF-κB), along with their cognate downstream effectors, the antioxidant enzymes (AOEs): manganese and copper zinc superoxide dismutase (MnSOD and CuZnSOD, respectively), catalase (CAT), glutathione peroxidase (GPx) and glutathione reductase (GLR). Results: MDD subjects exhibited higher levels of Nrf2 and its regulator Keap1, as well as NF-κB in the cytoplasm of PBMC compared to controls. This state was further reflected by increased levels of MnSOD, CuZnSOD and CAT proteins and by the lack of correlation between MnSOD and CAT, which could indicate impaired oxidative detoxification capacity in MDD patients. Moreover, increased levels of MnSOD, CuZnSOD and CAT in MDD patients positively correlated with levels of Nrf2, while increased levels of SODs were also positively related to NF-κB. There were no differences regarding the levels of GPx and GLR proteins, but the ratio of GLR/GPx was reduced, suggesting diminished capacity of GPx in antioxidative defence in PBMC of MDD subjects. Conclusion: These data provide evidence that MDD is characterized by up-regulation of redox-sensitive transcriptional factors (Nrf2 and NF-κB) and AOEs (MnSOD, CuZnSOD and CAT), indicating pro-oxidative state in the PBMC of MDD patients.

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Section: Resultsmentioning

confidence: 99%

Lymphocyte Levels of Redox-Sensitive Transcription Factors and Antioxidative Enzymes as Indicators of Pro-Oxidative State in Depressive Patients

Lukić¹,

Mitić²,

Djordjević³

et al. 2014

Neuropsychobiology

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“…Vascular aging and hypertension are associated with an increase in reactive species as suggested by numerous data [8,26]. Hydrogen peroxide is chemically more stable than other reactive species and can permeate through the vascular wall.…”

Section: Discussionmentioning

confidence: 94%

Relationship between catalase haplotype and arterial aging

et al. 2013

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“…40,41 Since RBCs are continuously oxygenated and their membranes are the target for the high reactivity of molecular O 2 and its reduction products, they possess various protective mechanisms to prevent the formation and the removal of various forms of ROS. These mechanisms are controlled in vivo by a wide spectrum of enzymatic 42,43 and non-enzymatic systems. [44][45][46] The antioxidant system protection depends on many factors such as hypoxia/reoxygenation 47 and this protection is more effective during hypoxia than during reoxygenation.…”

Section: Discussionmentioning

confidence: 99%

Circadian (about 24-hour) variation in malondialdehyde content and catalase activity of mouse erythrocytes

Sani

Sebaï²,

Ghanem-Boughanmi³

et al. 2014

Redox Report

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Lipid peroxidation is a part of normal metabolism that may cause biological molecule damage leading to the formation of several specific metabolites that include aldehydes of variable chains, such as malondialdehyde (MDA). These biological effects are controlled in vivo by a wide spectrum of enzymatic and non-enzymatic defense mechanisms among which catalase (CAT) is considered as an important regulator of oxidative stress. The present study aimed to investigate the possible relationship between the temporal patterns of the formation of MDA and the activity of CAT in the erythrocytes of mice. Twenty-four-hour studies were performed on male Swiss albino mice, 12 weeks old, synchronized to a 12:12 light: dark cycle for 3 weeks. Different and comparable groups of animals (n = 10) were sacrificed at an interval of 4 hours (1, 5, 9, 13, 17, and 21 hours after light onset (HALO)). The levels of erythrocyte MDA concentration and CAT activity both significantly (analysis of variance: F = 6.4, P < 0.002) varied according to the time of sampling under non-stressed conditions. The characteristics of the waveform describing the temporal patterns differed between the two studied variables, e.g. MDA content showing one peak (≅21 HALO) and CAT activity showing three peaks (≅9, 17, and 21 HALO). Cosinor analysis revealed a significant (adjusted Cosinor: P ≤ 0.018) circadian (τ ≅ 24 hours) rhythm in MDA level and no statistically significant rhythmicity in CAT activity. The differences and the absence of correlation between the curve patterns of erythrocyte MDA content and CAT activity under physiological conditions are hypothesized to explain that variation in lipid peroxidation may depend on several factors. Moreover, the identification of peak/trough levels of MDA accumulation in erythrocytes may reflect the degree of oxidative stress in these blood cells. In addition, the observed significant time-of-day effect suggests that, in both clinical and scientific settings, appropriate comparison of MDA production and CAT activity levels can only be achieved on data obtained at the same time of day.

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Age-related changes in an antioxidant defense system in elderly patients with essential hypertension compared with healthy controls

Cited by 50 publications

References 47 publications

Lymphocyte Levels of Redox-Sensitive Transcription Factors and Antioxidative Enzymes as Indicators of Pro-Oxidative State in Depressive Patients

Lymphocyte Levels of Redox-Sensitive Transcription Factors and Antioxidative Enzymes as Indicators of Pro-Oxidative State in Depressive Patients

Relationship between catalase haplotype and arterial aging

Circadian (about 24-hour) variation in malondialdehyde content and catalase activity of mouse erythrocytes

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