Age-Related Change in the Reducible Cross-Link of Human Skin and Aorta Collagens

Abstract

Age-related changes in the reducible cross-links of soluble and insoluble collagens from the human skin and aorta were evaluated. Histidinohydroxymerodesmosine decreased with age up to the fifth decade and then remained constant in the insoluble skin collagen but could not be detected in the insoluble aorta collagen. Dihydroxylysinonorleucine could be observed, if in a small amount, in the insoluble aorta collagen but not in both the soluble and insoluble skin collagens. Hydroxylysinonorleucine seemed to be present in only a small amount, although coeluting unknown substances made it difficult to evaluate correctly. Two unknown components decreasing with age were found; the one in the insoluble skin collagen and the other in the insoluble aorta collagen. Difference in reducible cross-link was indicated between the skin and aorta collagens.

Bateman purpura (dermatoporosis): a localized scurvy treated by topical vitamin C – double‐blind randomized placebo‐controlled clinical trial

“…Bateman purpura recorded by Bateman in 1836 is a classical sign of photo‐ageing, characterized by haemorrhage areas by the presence of purpuric eruptions like a petechial or confluent ecchymoses, by stellar scars, and a fragile skin due to thinness of the dermis. The location of such particular skin fragility is mainly represented by the exposed part of the forearms, legs, back of hands with dark purple blotches . In 2007, Kaya and Saurat proposed the term (dermatoporosis) to cover different manifestations and implications of the chronic cutaneous insufficiency/fragility syndrome.…”

Can proteomics yield insight into aging aorta?

“…SPARC‐like protein 1 is a member of the SPARC family that is known to modulate cell adhesion, proliferation, extracellular matrix deposition, and tissue remodeling . It has been shown that collagen I and III crosslink and increase in the arterial walls with aging . In this study, collagen alpha 1 and 2 were observed to be less abundant and the authors suggested that was caused by excessive collagen crosslinking and reduced solubility .…”

“…), suggesting the extracellular space subproteome is more reflective of aging effects than that of other parts of the aorta. Interestingly, 26 of these proteins are directly linked to the Ang II signaling cascade . Although, proteins with abundance changes also play significant roles in other cellular pathways, including a network of cell movement with a group of secreted extracellular proteins, apoptosis/cell cycle/proliferation, cytoskeletal, and extracellular structure, etc., which are important for vascular remodeling in aging .…”

“…The cytoskeleton proteins found to change abundance in aged aorta include RalA‐binding protein 1, STE20‐like serine/threonine‐protein kinase, calponin isoforms 1 and 3. RalA‐binding protein 1 interacts with the GTP‐bound form of many kinases, and can inhibit CDC42 GTPase activity that promotes cellular lamellipodia formation . STE20‐like serine/threonine‐protein kinase, which is less abundant in aged aorta , promotes actin filament/microtubule instability.…”

Vitamin C, Aged Skin, Skin Health

“…The lesions appeared mainly along the outside of the forearm in successive dark purple blotches of an irregular form and various magnitudes. The lesions are also localized to legs, back of the hands, bridge of the nose in subjects wearing glasses, and on the parts of the body where the skin is lax and inelastic [90]. These are flat, irregular, and purple lesions which appear on the skin as one gets older.…”