Age-Related Cell Proliferation and Apoptosis in the Kidney of Male Fischer 344 Rats With Observations on a Spontaneous Tubular Cell Adenoma

Abstract

Cell proliferation rate and apoptosis were examined in archival kidneys from young, middle-aged, and old male F344 rats. Immunohistochemical expression of proliferating cell nuclear antigen (PCNA) and apoptosis were quantified in the same cell populations of the proximal tubule epithelium. A total of 79 kidneys from 40 rats were examined. There was a progressive increase in cell proliferation rates in rats from 4 and 6-10 months of age. In 23-month-old rats, proliferative activity appeared to be reduced. No agerelated variations in apoptotic indices were found. One of the 16 rats aged 23 months had a tubular cell adenoma. In the tumoraffected kidney, cell proliferation rate was dramatically higher than in the contralateral kidney as well as in all the other kidneys examined. This high proliferative activity was not balanced by variation in cell death.

The Effect of Chronic Progressive Nephropathy on the Incidence of Renal Tubule Cell Neoplasms in Control Male F344 Rats

“…It stands to reason and has been shown that there is increased cell proliferation in kidneys with more severe CPN (3,15). Also, in a recent study, indices associated with increased cell proliferation were noted in the kidney of a male F344 rat bearing a unilateral renal tubule neoplasm as compared to the contralateral kidney (35).…”

“…It stands to reason and has been shown that there is increased cell proliferation in kidneys with more severe CPN (3,15). Also, in a recent study, indices associated with increased cell proliferation were noted in the kidney of a male F344 rat bearing a unilateral renal tubule neoplasm as compared to the contralateral kidney (35). If there were a known causal relationship between exacerbation of CPN and occurrence of RTCNs, some of the increases observed in NTP studies might be considered secondary to the exacerbated CPN rather than a potential direct effect of the chemical.…”

Age-dependent changes in cell proliferation and cell death in the periodontal tissue and the submandibular gland in mice: a comparison with other tissues and organs

“…In this study, we progressive cell proliferation of rat kidney in young-middle age, and then the proliferative activity decreased in old age. Meanwhile, no age-related variation was found in the apoptotic indices of rats (Cardani and Zavanella 2000). Another report indicated that the cell proliferation of renal glomeruli in rats was limited to the early stage of aging in diabetic rats (Murata et al 2002).…”

The Healthy, Aging, and Diseased Kidney: Relationship with Cardiovascular Disease

“…A small fraction of the renal cells have the ability for proliferation and repair, which plays a crucial role in tissue turnover and regeneration. This fraction of proliferative cells declines with age, further impairing the ability of the kidney to recover in response to an injury 15 . Growth factors, such as epidermal growth factor, insulin‐like growth factor‐1, and vascular endothelial growth factor, play important roles in kidney repair by offsetting proapoptotic cytokines 16 .…”