Age-related arabinoxylan hydrolysis and fermentation in the gastrointestinal tract of broilers fed wheat-based diets

Bautil, An; Verspreet, Joran; Buyse, Johan; Goos, Peter; Bedford, M. R.

doi:10.3382/ps/pez159

Cited by 60 publications

(74 citation statements)

References 55 publications

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“…The composition of the basal wheat-based diet used in this study is identical to that described in the study by Bautil et al. (2019) ( Supplementary Table 1 ).…”

Section: Methodsmentioning

confidence: 99%

“…To determine the dry matter ( DM ) content of feed and digesta samples, feed moisture content was assessed after 15 h of drying at 130°C in an air oven. Moisture contents of digesta samples were assessed after drying in an air oven using the temperature scheme as previously described by Bautil et al. (2019) .…”

Section: Methodsmentioning

confidence: 99%

“…Analysis of WE-AX and total arabinoxylan ( TOT-AX ) content was performed on feed samples and pooled, freeze-dried, and homogenized digesta samples from the ileum, caeca, and feces. Quantification of the total amount of arabinose and xylose (after hydrolysis) in these samples was performed by gas chromatography according to the method previously described by Bautil et al. (2019) .…”

Section: Methodsmentioning

confidence: 99%

“…The Xylazyme AX method (Megazyme, Bray, Ireland) was used to measure the grain-associated endoxylanase activity in the experimental diets. This grain-associated endoxylanase activity was determined using a similar procedure as the one described in detail by Bautil et al. (2019) .…”

Section: Methodsmentioning

confidence: 99%

“…The procedures for measuring microbial endoxylanase and arabinofuranosidase activities in ileal and caecal samples were optimized and described by Bautil et al. (2019) .…”

Section: Methodsmentioning

confidence: 99%

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Arabinoxylan-oligosaccharides kick-start arabinoxylan digestion in the aging broiler

Bautil¹,

Verspreet²,

Buyse

et al. 2020

Poultry Science

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While arabinoxylans ( AX ), an important dietary fiber fraction of wheat-based broiler diets, are known for exerting antinutritional effects in the gastrointestinal ( GI ) tract of broilers, the prebiotic potential of arabinoxylan-oligosaccharides ( AXOS ) is also well-documented. However, inconsistent performance responses as well as the effectiveness of low amounts of AXOS used in diets of previously conducted experiments put into question the classical prebiotic route being the sole mode of action of AXOS. The objective of this study was to investigate the effects of dietary AXOS addition on the rate of AX digestion in the gastrointestinal tract of broilers as a function of broiler age to gain more insight into the mode of action of these oligosaccharides. A feeding trial was performed on 480 one-day-old chicks (Ross 308) receiving a wheat-based diet supplemented with or without 0.50% AXOS, containing no endoxylanases. Digesta samples from ileum and caeca and fecal samples were analyzed for AX content, AX digestibility, intestinal viscosity, and microbial AX-degrading enzyme activities at 6 different ages (day 5, 10, 15, 21, 28, 35). Chicks fed from hatching with 0.50% AXOS demonstrated a higher ileal viscosity ( P < 0.05). Also higher levels of AX solubilization and fermentation compared to control birds at 10 D were observed. This was noted by the higher total tract AX digestibility of water-extractable AX (WE-AX) and total AX (TOT-AX) at this age ( P < 0.05). Although no significant difference in AX-degrading enzyme activities was observed among the dietary treatments, AXOS supplementation in young broilers was shown to stimulate or “kick-start” dietary AX digestion, thereby speeding up the development of a fiber-fermenting microbiome in the young broiler. This stimulation effect of AXOS could enable greater functional value to be extracted from dietary fiber in broiler feeds.

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“…The composition of the basal wheat-based diet used in this study is identical to that described in the study by Bautil et al. (2019) ( Supplementary Table 1 ).…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

“…The procedures for measuring microbial endoxylanase and arabinofuranosidase activities in ileal and caecal samples were optimized and described by Bautil et al. (2019) .…”

Section: Methodsmentioning

confidence: 99%

See 3 more Smart Citations

Arabinoxylan-oligosaccharides kick-start arabinoxylan digestion in the aging broiler

Bautil¹,

Verspreet²,

Buyse

et al. 2020

Poultry Science

Self Cite

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The role of feed enzymes in maintaining poultry intestinal health

Bedford

Apajalahti

2021

J Sci Food Agric

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Gut health or intestinal health is frequently discussed without any clear definition as to its meaning. It is suggested that this should be defined as intestinal integrity and functionality as both are a pre-requisite for the health of the intestine itself and the host. The health of the intestine is dependent upon a successful evolution of the absorptive capacity of the intestine, which in turn is influenced by the co-evolution of the intestinal immune systems and the microbiota. Nutrient supply plays a significant role in this process and from the perspective of the microbiota this changes with age as the intestines and upper gastrointestinal tract (GIT) microbiota become more effective in nutrient removal. Feed enzymes play a significant role in this process. Phytases can improve digestion of minerals, amino acids and energy and as a result reduce the availability of nutrients in the lower intestines for the microbiota. Protease can have a similar effect with amino acid supply. Non-starch polysaccharidases (NSPases) have a unique role in that they not only improve diet digestibility from the hosts perspective, thus limiting nutrient supply to the microbiota, but they also release soluble fragments of fibre from the insoluble matrix and/or depolymerize high molecular weight viscous fibre fractions in to smaller, more fermentable carbohydrate fractions. This results in a more favourable balance between fermentable carbohydrate to protein supply, a ratio which is deemed critical to maintaining good intestinal health. The dynamic nature of this complex evolution needs greater consideration if antibiotic free production is to succeed.

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