Age-Related Alterations of Hyaluronan and Collagen in Extracellular Matrix of the Muscle Spindles

Fan, Chenglei; Pirri, Carmelo; Fede, Caterina; Guidolin, Diego; Biz, Carlo; Petrelli, Lucía; Porzionato, Andrea; Macchi, Veronica; De, Raffaele; Stecco, Carla

doi:10.3390/jcm11010086

Cited by 10 publications

(4 citation statements)

References 56 publications

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“…Moreover, HA is abundant and randomly distributed in the first part of gestation (24th-29th), while in the second part of gestation (36th-40th) it was found to be distributed longitudinally within the whole layers of the fascial structures studied. These findings are consistent with those of other studies that reported the crucial role of HA in the fascial layers, permitting their gliding during the movement and the feature to reduce it with the age [24,25].…”

Section: Discussionsupporting

confidence: 93%

Fetal Fascial Reinforcement Development: From “a White Tablet” to a Sculpted Precise Organization by Movement

Pirri

Petrelli

Pérez‐Bellmunt

et al. 2022

Biology

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Fasciae have received much attention in recent years due to their important role in proprioception and muscular force transmission, but few studies have focused on fetal fasciae development and there is no study on the retinacula. The latter are fascial reinforcements that play a key role in proprioception and motor coordination. Furthermore, it is still unclear if they are genetically determined or if they are defined by movements, and if they are present during gestation or if they appear only later in the childhood. We aim to identify their structural organization by qualitative and quantitative assessments to establish their role the myofascial development, highlighting their appearance and organization. Samples from the wrist retinacula, posterior forearm, ankle retinacula, anterior leg, iliotibial tract and anterior thigh of six fetus body donors (from 24th to 40th week of gestation) and histological sections were obtained and a gross anatomy dissection was performed. Sections were stained with hematoxylin-eosin to observe their overall structure and measure their thicknesses. Using Weigert Van Gieson, Alcian blue and immunostaining to detect Hyaluronic Acid Binding Protein (HABP), Collagens I and III (Col I and III) were realized to assess the presence of elastic fibers and hyaluronan. This study confirms that the deep fasciae initially do not have organized layers and it is not possible to highlight any reinforcement. The fascial development is different according to the various area: while the deep fascia and the iliotibial tract is already evident by the 27th week, the retinacula begin to be defined only at the end of pregnancy, and their complete maturation will probably be reached only after birth. These findings suggest that the movement models the retinacula, structuring the fascial system, in particular at the end of pregnancy and in the first months of life. The fasciae can be imagined, initially, as “white tablets” composed of few elastic fibers, abundant collagens and HA, on which various forces, u movements, loads and gravity, “write their history”.

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Section: Discussionsupporting

confidence: 93%

Fetal Fascial Reinforcement Development: From “a White Tablet” to a Sculpted Precise Organization by Movement

Pirri

Petrelli

Pérez‐Bellmunt

et al. 2022

Biology

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“…In addition, inactivity and sedentary behaviors in the elderly cause a decline in skeletal muscle metabolism, together with a reduction of the ability of motor learning, range of motion and new locomotor patterns [ 45 ]. The same age-related process was demonstrated in the ECM of muscle spindles in mice samples: the increased stiffness partly explains the peripheral mechanisms underlying the age-related decline of proprioception and motor coordination [ 46 ].…”

Section: Discussionmentioning

confidence: 87%

The Effects of Aging on the Intramuscular Connective Tissue

Fede

Fan

Pirri

et al. 2022

IJMS

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The intramuscular connective tissue plays a critical role in maintaining the structural integrity of the muscle and in providing mechanical support. The current study investigates age-related changes that may contribute to passive stiffness and functional impairment of skeletal muscles. Variations in the extracellular matrix in human quadriceps femoris muscles in 10 young men, 12 elderly males and 16 elderly females, and in the hindlimb muscles of 6 week old, 8 month old and 2 year old C57BL/6J male mice, were evaluated. Picrosirius red, Alcian blue and Weigert Van Gieson stainings were performed to evaluate collagen, glycosamynoglycans and elastic fibers. Immunohistochemistry analyses were carried out to assess collagen I, collagen III and hyaluronan. The percentage area of collagen was significantly higher with aging (p < 0.01 in humans, p < 0.001 in mice), mainly due to an increase in collagen I, with no differences in collagen III (p > 0.05). The percentage area of elastic fibers in the perimysium was significantly lower (p < 0.01) in elderly men, together with a significant decrease in hyaluronan content both in humans and in mice. No significant differences were detected according to gender. The accumulation of collagen I and the lower levels of hyaluronan and elastic fibers with aging could cause a stiffening of the muscles and a reduction of their adaptability.

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“…AOD = integrated optical density/area. 35 For the immunofluorescence analysis, the sections were deparaffinized and blocked with 5% BSA solution for 30 min. Then, the slides were treated with the primary antibody solution at 4 °C overnight.…”

Section: Histological and Immunological Analysismentioning

confidence: 99%

Chitosan/Alginate Nanoparticles with Sustained Release of Esculentoside A for Burn Wound Healing

Zhu

Shao

et al. 2023

ACS Appl. Nano Mater.

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Burn injury remains one of the most devastating burdens on global public health. The inflammation, caused by burn injury and transplantation of dermal scaffolds, often leads to delayed burn wound healing. Esculentoside A (EsA), with a strong anti-inflammatory capacity, is an available agent that might contribute to the treatment of burn wounds. However, the poor stability and toxicity of EsA limit its clinical application. In the present study, we constructed chitosan/alginate nanoparticles (EsA-CS/ALG-NPs) to improve sustainability and reduce toxicity followed by impregnation of the prepared EsA-CS/ALG-NPs into a collagen/chitosan scaffold (EsA-CS/ALG-NPs@CCS). The particle size, structural morphology, thermal properties, and chemical interaction of repaired nanoparticles were evaluated using the Nanometrics instrument, differential scanning calorimetry, transmission electron microscopy, and Fourier transform infrared spectroscopy, respectively. The hybrid EsA-CS/ALG-NPs@CCS was evaluated for physical characteristics, in vitro drug release, biocompatibility, and anti-inflammation capacity with RAW 264.7 cells and in vivo burn wound healing studies with SD rats. The results showed that we successfully constructed CS/ALG-NPs and optimized the preparation process to achieve the highest encapsulation efficiency. The hybrid EsA-CS/ALG-NPs@CCS, with reduced cytotoxicity and sustained release of EsA, could alleviate inflammation, decrease the ratio of M1 macrophages, and increase the proportion of M2 macrophages in vitro. It was demonstrated that 5 μg EsA CS/ALG-NPs@CCS not only reduces inflammatory cytokines secretion and inhibits M1 macrophages but also promotes the release of anti-inflammatory cytokines and activates M2 macrophages, thereby achieving accelerated and high-quality healing of burn wounds ultimately. In summary, our work suggests that the synergistic combination of EsA, nanoparticles, and scaffolds provided a promising strategy for treating burn injuries.

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Age-Related Alterations of Hyaluronan and Collagen in Extracellular Matrix of the Muscle Spindles

Cited by 10 publications

References 56 publications

Fetal Fascial Reinforcement Development: From “a White Tablet” to a Sculpted Precise Organization by Movement

Fetal Fascial Reinforcement Development: From “a White Tablet” to a Sculpted Precise Organization by Movement

The Effects of Aging on the Intramuscular Connective Tissue

Chitosan/Alginate Nanoparticles with Sustained Release of Esculentoside A for Burn Wound Healing

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