Age Modulates Fe3O4Nanoparticles Liver Toxicity: Dose-Dependent Decrease in Mitochondrial Respiratory Chain Complexes Activities and Coupling in Middle-Aged as Compared to Young Rats

Baratli, Yosra; Charles, Anne-Laure; Wolff, Valérie; Tahar, Lotfi Ben; Smiri, Leila Samia; Bouitbir, Jamal; Zoll, Joffrey; Sakly, Mohsen; Auger, Cyril; Vogel, Thomas; Abdelmelek, Hafedh; Tebourbi, Olfa; Gény, Bernard

doi:10.1155/2014/474081

Cited by 29 publications

(23 citation statements)

References 52 publications

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“…Considering the aging of the active working population, the appropriate management of occupational risks in balance with emerging age-related functional limitations appears a challenging issue. Age has been reported to modify the susceptibility of rat liver mitochondria to iron (II,III) oxide (Fe 3 O 4 )-NPs which impaired all complexes of the mitochondrial respiration chain in middle aged animals (18 month-old), but not in young rats (3 month-old) (Baratli et al, 2013, 2014). Several mechanisms might explain these results, like increased fragility of older mitochondria and an excessive iron accumulation considered a feature of the aging process that becomes a potential causative factor of age-related mitochondrial dysfunction under conditions of cellular stress (Baratli et al, 2014).…”

Section: Resultsmentioning

confidence: 99%

“…Age has been reported to modify the susceptibility of rat liver mitochondria to iron (II,III) oxide (Fe 3 O 4 )-NPs which impaired all complexes of the mitochondrial respiration chain in middle aged animals (18 month-old), but not in young rats (3 month-old) (Baratli et al, 2013, 2014). Several mechanisms might explain these results, like increased fragility of older mitochondria and an excessive iron accumulation considered a feature of the aging process that becomes a potential causative factor of age-related mitochondrial dysfunction under conditions of cellular stress (Baratli et al, 2014). Unlike young and adult rats, old animals were more sensitive to cardiovascular and respiratory alterations induced by inhalation of SiO 2 -NPs (Chen et al, 2008).…”

Section: Resultsmentioning

confidence: 99%

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Biomarkers of susceptibility: State of the art and implications for occupational exposure to engineered nanomaterials

Iavicoli

Leso

Schulte

2016

Toxicology and Applied Pharmacology

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Rapid advances and applications in nanotechnology are expected to result in increasing occupational exposure to nano-sized materials whose health impacts are still not completely understood. Scientific efforts are required to identify hazards from nanomaterials and define risks and precautionary management strategies for exposed workers. In this scenario, the definition of susceptible populations, which may be at increased risk of adverse effects may be important for risk assessment and management. The aim of this review is to critically examine available literature to provide a comprehensive overview on susceptibility aspects potentially affecting heterogeneous responses to nanomaterials workplace exposure. Genetic, genotoxic and epigenetic alterations induced by nanomaterials in experimental studies were assessed with respect to their possible function as determinants of susceptibility. Additionally, the role of host factors, i.e. age, gender, and pathological conditions, potentially affecting nanomaterial toxicokinetic and health impacts, were also analysed. Overall, this review provides useful information to obtain insights into the nanomaterial mode of action in order to identify potentially sensitive, specific susceptibility biomarkers to be validated in occupational settings and addressed in risk assessment processes. The findings of this review are also important to guide future research into a deeper characterization of nanomaterial susceptibility in order to define adequate risk communication strategies. Ultimately, identification and use of susceptibility factors in workplace settings has both scientific and ethical issues that need addressing.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Biomarkers of susceptibility: State of the art and implications for occupational exposure to engineered nanomaterials

Iavicoli

Leso

Schulte

2016

Toxicology and Applied Pharmacology

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“…This slight difference probably resides in the intrinsic distinct characteristics of these two cell lines, in a different p53 status. 23 However, a recent study indicated that Fe 3 O 4 NPs specifically impair liver mitochondrial coupling in middleaged rats but not in young animals, 24 since SK-Hep-1 is a poor-differentiated hepatoma cell line, while Hep3B is a well-differentiated hepatoma cell line, 25 suggesting that these differences of Fe 3 O 4 NPs for mitochondrial function inhibition are associated with the differentiation of cells.…”

Section: Discussionmentioning

confidence: 99%

Size-dependent cytotoxicity of Fe3O4 nanoparticles induced by biphasic regulation of oxidative stress in different human hepatoma cells

Xie

Liu

Cai³

et al. 2016

IJN

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The application of Fe3O4 nanoparticles (NPs) has made great progress in the diagnosis of disease and in the drug delivery system for cancer therapy, but the relative mechanisms of potential toxicity induced by Fe3O4 have not kept pace with its development in the application, which has hampered its further clinical application. In this article, we used two kinds of human hepatoma cell lines, SK-Hep-1 and Hep3B, to investigate the cytotoxic effects and the involved mechanisms of small Fe3O4 NPs with different diameters (6 nm, 9 nm, and 14 nm). Results showed that the size of NPs effectively influences the cytotoxicity of hepatoma cells: 6 nm Fe3O4 NPs exhibited negligible cytotoxicity and 9 nm Fe3O4 NPs affected cytotoxicity via cellular mitochondrial dysfunction and by inducing necrosis mediated through the mitochondria-dependent intracellular reactive oxygen species generation. Meanwhile, 14 nm Fe3O4 NPs induced cytotoxicity by impairing the integrity of plasma membrane and promoting massive lactate dehydrogenase leakage. These results explain the detailed mechanism of different diameters of small Fe3O4 NPs-induced cytotoxicity. We anticipate that this study will provide different insights into the cytotoxicity mechanism of Fe3O4 NPs, so as to make them safer to use in clinical application.

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“…To promote its safe application, especially as a clinical agent, any potential nanotoxicity should be thoroughly and carefully evaluated. Some important nanotoxicities of FeNPs have been therefore discovered, such as inductions of cell inflammation [7], mitochondrial injury [8][9][10], detriment to cell viability [9,11], reactive oxygen species (ROS) [8,9,12,13], apoptosis [9,14,15], oxidative stress [15][16][17], cell motility impairment [12], autophagy [8,9], and DNA damage [16,17].…”

Section: Introductionmentioning

confidence: 99%

Research on Nanotoxicity of an Iron Oxide Nanoparticles and Potential Application

Zhang¹,

Xie²,

Liu³

et al. 2017

Toxicol Open Access

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The iron oxide nanoparticles (FeNPs) are widely used in biomedicine for good biocompatibility. To promote its safe application, any potential nanotoxicity should be thoroughly and carefully investigated. This paper systematically summarizes our lab's research on the nanotoxicity of iron oxide nanoparticles coated with dimercaptosuccinic acid (DMSA), including the effects of FeNPs on viability, apoptosis, cycle, and oxidative stress at cell level. In vitro studies revealed that the FeNPs showed obvious apoptosis of human acute monocyte cells (THP-1) and human hepatoma cells (HepG2) at the highest concentration. FeNPs resulted in common and cell typespecific nanotoxicities of the FeNPs to both human and mouse cells at the gene, disturbed cell's iron and osmosis homeostasis by the internalization of FeNPs through releasing iron ion in cells, resulted in cytotoxicity of DMSA as coating molecules of FeNPs and the inhibitor of DNA binding/differentiation (Id) related nanotoxicity of FeNPs at gene level. The studies of our lab shed many new insights into the nanotoxicity of the nanoparticle. Furthermore, the toxicity may play more value if it is guided and applied reasonably, such as iron supplement, anti-oxidation, and immunotherapy.

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Age Modulates Fe₃O₄Nanoparticles Liver Toxicity: Dose-Dependent Decrease in Mitochondrial Respiratory Chain Complexes Activities and Coupling in Middle-Aged as Compared to Young Rats

Cited by 29 publications

References 52 publications

Biomarkers of susceptibility: State of the art and implications for occupational exposure to engineered nanomaterials

Biomarkers of susceptibility: State of the art and implications for occupational exposure to engineered nanomaterials

Size-dependent cytotoxicity of Fe<sub>3</sub>O<sub>4</sub> nanoparticles induced by biphasic regulation of oxidative stress in different human hepatoma cells

Research on Nanotoxicity of an Iron Oxide Nanoparticles and Potential Application

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