Age‐induced changes in cellular membranes of imbibed soybean seed axes

Senaratna, Tissa; Gusse, Jenny F.; McKersie, Bryan D.

doi:10.1111/j.1399-3054.1988.tb09197.x

Cited by 72 publications

(44 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Also, the analysis of the fatty acid composition in the phospholipids does not indicate that lipid peroxidation occurs to any considerable extent during storage at 75% RH. This observation is in agreement with the results of others (Priestley and Leopold, 1979;Senaratna et al, 1988;Van Bilsen and Hoekstra, 1993;De Vos et al, 1994). However, during storage at 75% RH free fatty acids in the neem seeds accumulated and the phospholipid content decreased -a phenomenon that has been observed in many other systems during (accelerated) ageing (Priestley and Leopold, 1979;Senaratna et al, 1988;Van Bilsen and Hoekstra, 1993;Van Bilsen et al, 1994).…”

Section: Discussionsupporting

confidence: 83%

Is oxidative stress involved in the loss of neem (Azadirachta indica) seed viability?

et al. 2000

View full text Add to dashboard Cite

Neem (Azadirachta indica) is a valuable multipurpose tree of tropical arid and semi-arid regions. The use of its seeds is hindered by their short storage longevity. The possible causes of rapid loss of viability were investigated on different seed lots during exposure to 32% and 75% RH at 20°C. Within 6 months the seeds almost lost germinability at 75% RH, whereas at 32% RH viability decreased only slightly. On rehydration, the axis cells from nongerminable seeds had lost turgor, whereas those from viable seeds were turgescent as visualized by low temperature scanning electron microscopy images of fractured axes. Glutathione oxidation status was used to estimate oxidative stress during storage. Oxidative stress was much higher at 75% RH storage than at 32% RH, mainly caused by the rapid loss of reduced glutathione at 75% RH. Oligosaccharides and phospholipids decreased, and free fatty acids increased during storage at the high RH but remained at a constant level at the low RH. However, the degree of fatty acid unsaturation between viable and nonviable seed lots was similar. During the (slow) dehydration of fresh seeds, total glutathione, oligosaccharides and phospholipids accumulated, particularly in the initially more hydrated seeds. We interpret this accumulation as a postmaturation process associated with acquisition of the capability for long-term survival in the dry state. The mass ratio of oligosaccharides to sucrose was 0.19 on average in dehydrated neem seeds. The data suggest that the storage behaviour of neem seeds has features that characterize it as orthodox.

show abstract

Section: Discussionsupporting

confidence: 83%

Is oxidative stress involved in the loss of neem (Azadirachta indica) seed viability?

et al. 2000

View full text Add to dashboard Cite

show abstract

“…We suppose that aging begins with the slight increase in the permeability of some cells, probably caused by an accumulation of free fatty acids in membranes (Senaratna et al, 1988). This would not cause seed death but could reduce seed vigor.…”

Section: How Can the R Value Be Lnterpreted?mentioning

confidence: 99%

An Electron Paramagnetic Resonance Spin-Probe Study of Membrane-Permeability Changes with Seed Aging

1997

View full text Add to dashboard Cite

We developed an electron paramagnetic resonance spin-probe technique to study changes in the barrier properties of plasma membranes in wheat (Triticum aestivum L.) seeds during aging under dry storage. l h e estimation of these barrier properties was based on the differential permeability of membranes for the stable free radical 4-0~0-2,2,6,6-tetramethyI-l-piperidinyloxy and the broadening agent ferricyanide. l h e line-height ratio between the water and lipid components in the electron paramagnetic resonance spectra of 4-0~0-2,2,6,6-tetramethyI-l -piperidinyloxy ( R value) allowed for the quantitative assessment of the plasma membrane permeability in small samples, enabling separate studies of the axis, scutellum, aleurone layer, and starchy endosperm tissue. High R values corresponded to low permeability and vice versa. Starchy endosperm cells had completely permeable plasma membranes even in mature, viable seeds. l h e loss of germinability with aging coincided with a considerably increased plasma membrane permeability of the embryo axis cells, but not of the scutellum and aleurone layer cells. l h e threshold R value for the individual axes associated with viability loss was established at 5 to 6 , with the total ranging from O to more than 12. We suggest that the R value of an individual axis is the result of contributions from all individual cells, each of them characterized by a different permeability. l h e loss of viability, therefore, corresponds to the accumulation of cells having permeability above a critical level.

show abstract

“…Slow drying over a 6-d period renders them desiccation tolerant from 18 DAP onward, whereas rapid dehydration over a 2-d period is tolerated only from 22 DAP onward. The loss of viability in desiccation-sensitive embryos is often attributed to the loss of plasmalemma integrity after drying, as deduced from the excessive leakage of cytoplasmic solutes (Senaratna et al, 1988;Blackman et al, 1995). Disruption of membrane structures may lead to decompartmentalization of the cellular components, resulting in the release of enzymes that degrade cytoplasmic structures.…”

mentioning

confidence: 99%

Fourier Transform Infrared Microspectroscopy Detects Changes in Protein Secondary Structure Associated with Desiccation Tolerance in Developing Maize Embryos1

Wolkers

Bochicchio²,

Selvaggi³

et al. 1998

Plant Physiology

View full text Add to dashboard Cite

Isolated immature maize (Zea mays L.) embryos have been shown to acquire tolerance to rapid drying between 22 and 25 d after pollination (DAP) and to slow drying from 18 DAP onward. To investigate adaptations in protein profile in association with the acquisition of desiccation tolerance in isolated, immature maize embryos, we applied in situ Fourier transform infrared microspectroscopy. In fresh, viable, 20-and 25-DAP embryo axes, the shapes of the different amide-I bands were identical, and this was maintained after flash drying. On rapid drying, the 20-DAP axes had a reduced relative proportion of ␣-helical protein structure and lost viability. Rapidly dried 25-DAP embryos germinated (74%) and had a protein profile similar to the fresh control axes. On slow drying, the ␣-helical contribution in both the 20-and 25-DAP embryo axes increased compared with that in the fresh control axes, and survival of desiccation was high. The protein profile in dry, mature axes resembled that after slow drying of the immature axes. Rapid drying resulted in an almost complete loss of membrane integrity in the 20-DAP embryo axes and much less so in the 25-DAP axes. After slow drying, low plasma membrane permeability ensued in both the 20-and 25-DAP axes. We conclude that slow drying of excised, immature embryos leads to an increased proportion of ␣-helical protein structures in their axes, which coincides with additional tolerance of desiccation stress.

show abstract

Age‐induced changes in cellular membranes of imbibed soybean seed axes

Cited by 72 publications

References 21 publications

Is oxidative stress involved in the loss of neem (Azadirachta indica) seed viability?

Is oxidative stress involved in the loss of neem (Azadirachta indica) seed viability?

An Electron Paramagnetic Resonance Spin-Probe Study of Membrane-Permeability Changes with Seed Aging

Fourier Transform Infrared Microspectroscopy Detects Changes in Protein Secondary Structure Associated with Desiccation Tolerance in Developing Maize Embryos1

Contact Info

Product

Resources

About