Age-induced and photoinduced changes in gene expression profiles in facial skin of Caucasian females across 6 decades of age

Kimball, Alexa B.; Alora-Palli, Maria B.; Tamura, Makio; Mullins, Lisa; Soh, Chieko; Binder, Robert L.; Houston, Neil; Conley, Emily Drabant; Tung, Joyce Y.; Annunziata, Nikki E.; Bascom, Charles C.; Isfort, Robert J.; Jarrold, Bradley B.; Kainkaryam, Raghu; Rocchetta, Heather L.; Swift, Dionne; Tiesman, Jay P.; Toyama, Kazumi; Xu, Jun; Yan, Xianghong; Osborne, Rosemarie

doi:10.1016/j.jaad.2017.09.012

Cited by 55 publications

(81 citation statements)

References 40 publications

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“…The biopsy specimens were processed for separation into epidermal and dermal compartments by laser capture microdissection (SI Appendix, SI Methods). Collection of the unordered samples from 152 Caucasian and 67 African-American females has been described previously (34). The mRNA target labeling and processing steps are described in detail in SI Appendix, SI Methods.…”

Section: Methodsmentioning

confidence: 99%

Population-level rhythms in human skin with implications for circadian medicine

Ruben

Schmidt

et al. 2018

Proc. Natl. Acad. Sci. U.S.A.

103

107

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Skin is the largest organ in the body and serves important barrier, regulatory, and sensory functions. The epidermal layer shows rhythmic physiological responses to daily environmental variation (e.g., DNA repair). We investigated the role of the circadian clock in the transcriptional regulation of epidermis using a hybrid experimental design, in which a limited set of human subjects (n = 20) were sampled throughout the 24-h cycle and a larger population (n = 219) were sampled once. We found a robust circadian oscillator in human epidermis at the population level using pairwise correlations of clock and clock-associated genes in 298 epidermis samples. We then used CYCLOPS to reconstruct the temporal order of all samples, and identified hundreds of rhythmically expressed genes at the population level in human epidermis. We compared these results with published time-series skin data from mice and found a strong concordance in circadian phase across species for both transcripts and pathways. Furthermore, like blood, epidermis is readily accessible and a potential source of biomarkers. Using ZeitZeiger, we identified a biomarker set for human epidermis that is capable of reporting circadian phase to within 3 hours from a single sample. In summary, we show rhythms in human epidermis that persist at the population scale and describe a path to develop robust single-sample circadian biomarkers.

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Section: Methodsmentioning

confidence: 99%

Population-level rhythms in human skin with implications for circadian medicine

Ruben

Schmidt

et al. 2018

Proc. Natl. Acad. Sci. U.S.A.

103

107

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show abstract

“…We used the microarray dataset from a previous population study of human epidermis and dermis [23]. This study recruited 154 Caucasian females (aged 20-74 y) in the U.S. Three punch biopsies were collected from each subject, with one biopsy per body site (arm, buttock and cheek).…”

Section: Comparing Clock Robustness Between Human Epidermis and Dermismentioning

confidence: 99%

“…To evaluate clock function in the broader population, we next analyzed clock gene expression from 154 subjects, aged 20-74 y. Each subject contributed three punch biopsies from cheek, forearm, and buttock [23]; sampling time was not recorded. To compare robustness of clock oscillations between different skin layers and body sites, we computed correlation matrices of clock and clock-associated genes [24] for each of the six conditions (e.g., epidermis-cheek, dermis-cheek, etc.).…”

Section: Clock Strength Varies Between Skin Layersmentioning

confidence: 99%

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A single-sample circadian biomarker that performs across populations and platforms

Ruben

Francey

et al. 2019

Preprint

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Background: For circadian medicine to influence health, such as when to take a drug or undergo a procedure, a practical way to measure body time is needed. Recent machine learning algorithms show that gene expression data from blood and skin can provide reliable estimates of body time. However, for clinical viability, a biomarker must be easily measured and generalizable to a broad population. It is not clear that any circadian biomarker yet satisfies these criteria. Results:We analyzed 24 h molecular rhythms in human dermis and epidermis at three distinct body sites, leveraging both longitudinal and population data. Circadian clock function was strongest in the epidermis, regardless of body site. We identified a 12-gene biomarker set that reported circadian phase to within 3 hours from a single sample of epidermis--the skin's most superficial layer. This set performed well across body sites, ages, sexes, and detection platforms. Conclusions:This research shows that the clock in epidermis is more robust than dermis regardless of body site. To encourage ongoing validation of this biomarker in diverse populations, diseases, and experimental designs, we developed SkinPhaser--a user-friendly app to test biomarker performance in datasets (https://github.com/gangwug/SkinPhaser).

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“…After 40-50 years, especially in the menopausal and postmenopausal period of women, changes in skin indices begin to be expressed, a deformational type of involutional changes is formed, ptosis, the oval of the face is deformed. Kimball and colleagues demonstrates a wide range of molecular processes in skin affected by aging, providing relevant targets for improving the condition of aging skin at different life stages and defining a molecular pattern of epidermal gene expression in women who appear younger than their chronologic age (18). On the other hand, men are a growing patient population in aesthetic medicine and are increasingly seeking minimally invasive cosmetic procedures.…”

Section: Groupsmentioning

confidence: 99%

Instrumental Assessment of the Face Skin Aging in Women

Мантурова

Stupin

Smirnova

et al. 2018

Serbian Journal of Experimental and Clinical Research

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The aim of this study is to conduct several non-invasive methods for assessing the level of circulatory disturbance, elasticity and aging of skin in patients of different age groups in order to expand the diagnostic capabilities and evaluate the effectiveness of current research in aesthetic medicine. Clinical and instrumental exploration of 160 women aged 17 to 75 years with varying degrees of involutional skin changes was carried out. To objectify the assessment of skin condition, in all group of patients modern instrumental methods were used, such as: elastometry, ultrasound examination of the skin, laser Doppler flowmetry, transcutaneous oxygen tension. Concurrent implementation of several non-invasive methods for assessing the level of circulatory disturbance, elasticity and aging of the skin, allowed us to find new possibilities for studying the functional state of the skin. These methods extend the possibilities of ultrasonic research methods used today in aesthetic cosmetology. The obtained comparative data of elastometry, ultrasonography, laser Doppler flowmetry and transcutaneous oximetry in patients of different age groups showed the presence of elasticity and structure defect, skin thickness and subcutaneous fat, as well as microcirculation changes since 25 years and marked changes after 40 years.

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Age-induced and photoinduced changes in gene expression profiles in facial skin of Caucasian females across 6 decades of age

Cited by 55 publications

References 40 publications

Population-level rhythms in human skin with implications for circadian medicine

Population-level rhythms in human skin with implications for circadian medicine

A single-sample circadian biomarker that performs across populations and platforms

Instrumental Assessment of the Face Skin Aging in Women

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