Age-dependent neuroectodermal differentiation capacity of human mesenchymal stromal cells: limitations for autologous cell replacement strategies

Hermann, Andreas; List, Catrin; Habisch, Hansjörg; Vukićević, Vladimir; Ehrhart‐Bornstein, Monika; Brenner, Rolf E.; Bernstein, Peter S.; Fickert, Stefan; Storch, Alexander

doi:10.3109/14653240903313941

Cited by 64 publications

(58 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This potential was also reported for MSCs [4][5][6]. If analyzed more precisely, MSCs often retain mesodermal transcriptome characteristics after transgerminal conversion and thus lack full transdifferentiation characteristics [7,8].…”

Section: Discussionmentioning

confidence: 87%

Perivascular Mesenchymal Stem Cells From the Adult Human Brain Harbor No Instrinsic Neuroectodermal but High Mesodermal Differentiation Potential

Lojewski

Srimasorn

Rauh

et al. 2015

Stem Cells Translational Medicine

Self Cite

View full text Add to dashboard Cite

Brain perivascular cells have recently been identified as a novel mesodermal cell type in the human brain. These cells reside in the perivascular niche and were shown to have mesodermal and, to a lesser extent, tissue-specific differentiation potential. Mesenchymal stem cells (MSCs) are widely proposed for use in cell therapy in many neurological disorders; therefore, it is of importance to better understand the "intrinsic" MSC population of the human brain. We systematically characterized adult human brain-derived pericytes during in vitro expansion and differentiation and compared these cells with fetal and adult human brain-derived neural stem cells (NSCs) and adult human bone marrowderived MSCs. We found that adult human brain pericytes, which can be isolated from the hippocampus and from subcortical white matter, are-in contrast to adult human NSCs-easily expandable in monolayer cultures and show many similarities to human bone marrow-derived MSCs both regarding both surface marker expression and after whole transcriptome profile. Human brain pericytes showed a negligible propensity for neuroectodermal differentiation under various differentiation conditions but efficiently generated mesodermal progeny. Consequently, human brain pericytes resemble bone marrow-derived MSCs and might be very interesting for possible autologous and endogenous stem cell-based treatment strategies and cell therapeutic approaches for treating neurological diseases. STEM CELLS

show abstract

Section: Discussionmentioning

confidence: 87%

Perivascular Mesenchymal Stem Cells From the Adult Human Brain Harbor No Instrinsic Neuroectodermal but High Mesodermal Differentiation Potential

Lojewski

Srimasorn

Rauh

et al. 2015

Stem Cells Translational Medicine

Self Cite

View full text Add to dashboard Cite

show abstract

“…Human umbilical cord perivascular cells (HUCPVCs), derived from the region surrounding the blood vessels within the human umbilical cord, are an attractive and readily available alternative source of mesenchymal stromal cells (MSCs) for cell therapy [1] and may overcome some of the limitations of bone marrow (BM)-derived MSCs, such as a decline in function with donor age [2,3]. Furthermore, HUCPVCs have a higher clonogenic frequency than BM-MSCs and contain selfrenewing stem cells with multilineage differentiation potential in vitro and in vivo [4].…”

Section: Introductionmentioning

confidence: 99%

Brief Report: The Potential Role of Epigenetics on Multipotent Cell Differentiation Capacity of Mesenchymal Stromal Cells

et al. 2012

View full text Add to dashboard Cite

Human umbilical cord perivascular cells (HUCPVCs) are a readily available source of mesenchymal stromal cells (MSCs) for cell therapy. We were interested in understanding how differences from human bone marrow (BM)-derived MSCs might yield insights into MSC biology. We found that HUCPVCs exhibited increased telomerase activity and longer telomeres compared with BM-MSCs. We also observed enhanced expression of the pluripotency factors OCT4, SOX2, and NANOG in HUCPVCs. The methylation of OCT4 and NANOG promoters was similar in both cell types, indicating that differences in the expression of pluripotency factors between the MSCs were not associated with epigenetic changes. MSC methylation at these loci is greater than reported for embryonic stem cells but less than in dermal fibroblasts, suggesting that multipotentiality of MSCs is epigenetically restricted. These results are consistent with the notion that the MSC population (whether BM-or HUCPV-derived) exhibits higher proliferative capacity and contains more progenitor cells than do dermal fibroblasts. STEM CELLS 2013;31:215-220 Disclosure of potential conflicts of interest is found at the end of this article.

show abstract

“…Por otra parte, estudios en animales han mostrado cambio en su función; los linfocitos TCD4 FoxP3 de ratones viejos producen más IL-10 y suprimen a la molécula CD80 (B7-1) en células dendríticas, lo que aumenta la supresión inmune. 55 Células madre Las células madre del mesénquima provenientes de ancianos, contrario a lo que sucede con las de jó-venes, no son capaces de diferenciarse in vitro a células neuroectodérmicas, 56 lo que limita la utilización de células autólogas de personas mayores, para reemplazo o regeneración de tejidos, más aún su donación.…”

Section: Células Tcd24cd25unclassified

Senescencia del sistema inmune y alteraciones relacionadas con el asma

Vega-Robledo

Rico-Rosillo

2017

RAM

View full text Add to dashboard Cite

Senescence is an irreversible process by which cells enter to a permanent cell cycle arrest with generalized molecular changes. Senescent cells remain metabolically active and most of them show a secretory phenotype; through its secretion may induce senescence or cancer in other cells. The secretory cells in the so-called transient senescence may participate in embryogenesis, tissue regeneration and immune response. The deleterious changes associated with age affect the immune system members and the immune senescence cause poor response to vaccines and susceptibility to cancer and infections. These latter are a frequent cause of asthma mostly in the elderly, the incidence is increasing in old people, and it may be related with those anatomical, physiological and immune changes caused by age, asthma chronicity and external agents. Comorbidity in the elderly worsens the ailment and hinders diagnosis, therefore, knowledge and handling of these clinical entities must be in control by the physicians responsible of the first level attention to old patients. Senescencia del sistema inmune y alteraciones relacionadas con el asma

show abstract

Age-dependent neuroectodermal differentiation capacity of human mesenchymal stromal cells: limitations for autologous cell replacement strategies

Cited by 64 publications

References 47 publications

Perivascular Mesenchymal Stem Cells From the Adult Human Brain Harbor No Instrinsic Neuroectodermal but High Mesodermal Differentiation Potential

Perivascular Mesenchymal Stem Cells From the Adult Human Brain Harbor No Instrinsic Neuroectodermal but High Mesodermal Differentiation Potential

Brief Report: The Potential Role of Epigenetics on Multipotent Cell Differentiation Capacity of Mesenchymal Stromal Cells

Senescencia del sistema inmune y alteraciones relacionadas con el asma

Contact Info

Product

Resources

About