Age dependent increase of elastase type protease activity in mouse skin

Labat‐Robert, J.; Fourtanier, Annie; Boyer-Lafargue, B; Robert, L.

doi:10.1016/s1011-1344(00)00085-3

Cited by 80 publications

(59 citation statements)

References 19 publications

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“…4,6,7,9,11,21,22,24,29 Moreover, gene chip analysis indicated a lot of similarities in the comparisons between young versus old skins and our control versus UVB-exposed skins, corroborating the validity of our photodamage or premature photoaging model. Taken together, the present study discloses a novel photodamage model with human skin xenograft that recapitulates mechanisms underlying morphological changes such as wrinkling reported to date, and can be used for the further study of photoaging.…”

Section: Discussionsupporting

confidence: 77%

“…5,8,10,11,20 On the other hand, it has been reported that the degeneration of these fibers in chronologically and/or photoaged skin stems from an increase in matrix metalloproteinase-12 (MMP-12) and/or elastase, an elastin-degrading enzyme that is produced and secreted by dermal fibroblasts. [21][22][23][24] To date, the mechanisms underlying the alteration of elastin fibers in photoaged skin including their production, accumulation, and degradation are not fully understood.…”

mentioning

confidence: 99%

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Mechanistic Effects of Long-Term Ultraviolet B Irradiation Induce Epidermal and Dermal Changes in Human Skin Xenografts

Hachiya

Sriwiriyanont

Fujimura

et al. 2009

The American Journal of Pathology

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UVB irradiation has been reported to induce photoaging and suppress systemic immune function that could lead to photocarcinogenesis. However, because of the paucity of an UVB-induced photodamaged skin model, precise and temporal mechanism(s) underlying the deleterious effects of long-term UVB exposure on human skin have yet to be delineated. In this study, we established a model using human skin xenografted onto severe combined immunodeficient mice, which were subsequently challenged by repeated UVB irradiation for 6 weeks. Three-dimensional optical image analysis of skin replicas and noninvasive biophysical measurements illustrated a significant increase in skin surface roughness, similar to premature photoaging, and a significant loss of skin elasticity after long-term UVB exposure. Resembling authentically aged skin, UVB-exposed samples exhibited significant increases in epithelial keratins (K6, K16, K17), elastins, and matrix metalloproteinases (MMP-1, MMP-9, MMP-12) as well as degradation of collagens (I, IV, VII). The UVB-induced deterioration of fibrous keratin intermediate filaments was also observed in the stratum corneum. Additionally, similarities in gene expression patterns between our model and chronologically aged skin substantiated the plausible relationship between photodamage and chronological age. Furthermore , severe skin photodamage was observed when neutralizing antibodies against TIMP-1 , an endogenous inhibitor of MMPs , were administered during the UVB exposure regimen. Taken together , these findings suggest that our skin xenograft model recapitulates premature photoaged skin and provides a comprehensive tool with which to assess the deleterious effects of UVB irradiation.

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Section: Discussionsupporting

confidence: 77%

mentioning

confidence: 99%

Mechanistic Effects of Long-Term Ultraviolet B Irradiation Induce Epidermal and Dermal Changes in Human Skin Xenografts

Hachiya

Sriwiriyanont

Fujimura

et al. 2009

The American Journal of Pathology

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“…The structural integrity of the ECM is compromised with the increase in the basal or induced levels of MMPs/elastase enzymes as well as their activity with aging or exposure to UV radiation. The ECM proteolytic enzymes (MMPs/elastases) are produced by epidermal keratinocytes, dermal fibroblasts, neutrophils and melanoma cells in the mediation of skin aging or cancer [3,[15][16][17][18]. P. leucotomos extract directly inhibits the activities of MMPs (MMP-1, 2, 3, 9) as well as the cellular expression of MMPs (MMP-1, 2) in nonirradiated or UV radiated dermal fibroblasts or epidermal keratinocytes [2,3].…”

Section: Discussionmentioning

confidence: 99%

Beneficial Regulation of Elastase Activity and Expression of Tissue Inhibitors of Matrixmetalloproteinases, Fibrillin, Transforming Growth Factor-β, and Heat Shock Proteins by P. leucotomos in Nonirradiated or Ultraviolet-Radiated Epidermal Keratinocytes

Philips

González

2013

ISRN Oxidative Medicine

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There is loss of the structural integrity of the extracellular matrix (ECM) with intrinsic aging as well as photoaging, largely due to reactive oxygen species (ROS). The structural ECM proteins include the microfibrils that are composed of fibrillin. The structural ECM proteins are primarily degraded by the matrixmetalloproteinases (MMPs) and elastase enzymes. The MMPs are inhibited by the tissue inhibitors of MMPs (TIMPs). A primary regulator of the ECM proteins is transforming growth factor-(TGF-), and the chaperone proteins important for its formation are the heat shock proteins (HSP). P. leucotomos extract beneficially regulates of MMPs, TIMPs, and TGF-in nonirradiated or ultraviolet (UV) radiated fibroblasts and melanoma cells. The hypothesis of this research was that the antioxidant activity or chemistry of P. leucotomos extract would also directly inhibit elastase activity, stimulate the cellular expression of TIMPs, fibrillins, and TGF-, and regulate HSPs in nonirradiated and UVA or UVB radiated epidermal keratinocytes. P. leucotomos directly inhibited elastase activity, stimulated the cellular expression of TIMPs, fibrillins, and TGF-, and differentially regulated HSPs in nonirradiated and UVA or UVB radiated epidermal keratinocytes. We infer that the P. leucotomos extract strengthens the ECM and is effective in the prevention or treatment of intrinsic and photoaging of skin.

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“…Further, the increase in the activity of collagenase, elastase, and HYAL is triggered by the high levels of reactive oxygen species (ROS) produced when the skin is exposed to excessive UV radiations leading to skin aging. [9][10][11] The skin shows hypertrophic features with deep wrinkles, leathery appearance, dark/light pigmentation, sallowness, premalignant lesions, irregular dryness, and lentigines. [12] Other signs of aging include elastosis (a coarse, yellow, cobblestoned effect is on the skin) and actinic purpura (easy bruising corresponding to vascular wall fragility in dermis).…”

Section: Clinical Manifestation Of Photoagingmentioning

confidence: 99%

Untitled

Garg

2017

IJGP

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Skin ageing is a complex multifactorial process occurring in all living beings. It generally comprises two independent and distinct processes, i.e., intrinsic, age-dependent or chronological aging and premature aging, or photoaging which is the result of undue exposure of the skin to ultraviolet (UV) radiations. Photoaging can be prevented or the effects of photoaging can be reduced by the use of certain anti-aging and anti-wrinkle agents. The extracellular matrix (ECM) which forms the outermost part of skin comprises fibroblasts and proteins such as collagen and elastin. Degradation of ECM is directly linked to skin aging and is responsible for the increase in activity of certain enzymes such as collagenase, elastase, and hyaluronidase (HYAL) that are involved in skin aging. With aging there is decrease in the levels of elastin, collagen, and hyaluronic acid which leads to loss of strength and flexibility of skin and appear as wrinkles on the skin. Further, the increase in the activity of collagenase, elastase, and HYAL is triggered by the high levels of reactive oxygen species produced when the skin is exposed to excessive UV radiations leading to skin aging. Today, in the age of modern science and advanced technology, although many techniques such as laser rejuvenation, plastic surgery, and lots of synthetic products such as sunscreen lotions, creams are available yet, there is a place for natural, herbal anti-aging cosmetics. Various botanical extracts have the power to reduce the appearance of skin aging and enhance the beauty of the skin. Medicinal plants have several phytoconstituents such as polyphenols, alkaloids, tannins, saponins, carotenoids, and terpenoids which possess antioxidant properties and can be used in treating the signs of aging. Some plants contain phenolic compounds which have free-radical scavenging property and can suppress aging. Some plants or their extracts possess several other constituents that may have the capability of inhibiting various enzyme such as HYAL, elastase, and matrix metalloproteinases enzyme that play a major role in skin aging. Some of them may possess the free radical scavenging or antioxidant property to fight against the signs of aging. This review focuses on the compilation of medicinal plants and natural compounds as anti-aging and anti-wrinkling agents along with their chemical constituents based on their possible molecular mechanism of action. The present review will facilitate the scientists working in this area for the development of new anti-aging and anti-wrinkle formulations with better efficiency and safety.

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Age dependent increase of elastase type protease activity in mouse skin

Cited by 80 publications

References 19 publications

Mechanistic Effects of Long-Term Ultraviolet B Irradiation Induce Epidermal and Dermal Changes in Human Skin Xenografts

Mechanistic Effects of Long-Term Ultraviolet B Irradiation Induce Epidermal and Dermal Changes in Human Skin Xenografts

Beneficial Regulation of Elastase Activity and Expression of Tissue Inhibitors of Matrixmetalloproteinases, Fibrillin, Transforming Growth Factor-β, and Heat Shock Proteins by P. leucotomos in Nonirradiated or Ultraviolet-Radiated Epidermal Keratinocytes

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