Age-dependent expression of VEGF isoforms and receptors in the rabbit anterior cruciate ligament

Hofstaetter, Jochen G.; Saad, Fawzy A.; Sunk, Ilse-Gerlinde; Bobacz, Klaus; Friehs, Ingeborg; Glimcher, Melvin J.

doi:10.1016/j.bbagen.2007.02.006

Cited by 14 publications

(17 citation statements)

References 33 publications

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“…Since an age-dependent expression of VEGF isoforms and receptors in rabbits has been demonstrated,10 we decided to also include in the present study young patients with asthma (YA) and young controls (≤40 years old) in order to evaluate potential differences related to age.…”

Section: Methodsmentioning

confidence: 99%

Expression of vascular remodelling markers in relation to bradykinin receptors in asthma and COPD

Ricciardolo

Sabatini

Sorbello

et al. 2013

Thorax

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Section: Methodsmentioning

confidence: 99%

Expression of vascular remodelling markers in relation to bradykinin receptors in asthma and COPD

Ricciardolo

Sabatini

Sorbello

et al. 2013

Thorax

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“…Real-time quantitative RT-PCR was used to evaluate mRNA levels using an ABI PRISM ® 7700 sequence detection system (Applied Biosystems, Foster City, CA, USA). We have previously described the real-time RT-PCR conditions (Hofstaetter et al ., 2004; Hofstaetter et al ., 2005; Hofstaetter et al ., 2007). Briefly, the reactions were run in triplicate, each containing 1 μl cDNA along with 2.5 pmoles of gene specific primers (Table 1) in a final volume of 25 μl with the following thermal profile: 5 min initial denaturing step to activate the DNA polymerase, then 40 cycles each at 95°C for 35 sec, 60°C for 45 sec, and 72°C for 45 sec.…”

Section: Methodsmentioning

confidence: 99%

Use of genetically modified muscle and fat grafts to repair defects in bone and cartilage

Evans

Liu

Glatt

et al. 2009

eCM

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We report a novel technology for the rapid healing of large osseous and chondral defects, based upon the genetic modification of autologous skeletal muscle and fat grafts. These tissues were selected because they not only possess mesenchymal progenitor cells and scaffolding properties, but also can be biopsied, genetically modified and returned to the patient in a single operative session. First generation adenovirus vector carrying cDNA encoding human bone morphogenetic protein-2 (Ad.BMP-2) was used for gene transfer to biopsies of muscle and fat. To assess bone healing, the genetically modified ("gene activated") tissues were implanted into 5mm-long critical size, mid-diaphyseal, stabilized defects in the femora of Fischer rats. Unlike control defects, those receiving gene-activated muscle underwent rapid healing, with evidence of radiologic bridging as early as 10 days after implantation and restoration of full mechanical strength by 8 weeks. Histologic analysis suggests that the grafts rapidly differentiated into cartilage, followed by efficient endochondral ossification. Fluorescence in situ hybridization detection of Y-chromosomes following the transfer of male donor muscle into female rats demonstrated that at least some of the osteoblasts of the healed bone were derived from donor muscle. Gene activated fat also healed critical sized defects, but less quickly than muscle and with more variability. Anti-adenovirus antibodies were not detected. Pilot studies in a rabbit osteochondral defect model demonstrated the promise of this technology for healing cartilage defects. Further development of these methods should provide ways to heal bone and cartilage more expeditiously, and at lower cost, than is presently possible.

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“…Vascular endothelial growth factor‐A (VEGF‐A, hereafter referred to as VEGF) is the most potent mediator of this neovascularization event in both health and disease [ 39 , 103 ]. The VEGF gene resides on chromosome 6 [ 167 and is organized as a single gene consisting of eight exons spanning approximately 14 kbp and separated by 7 introns [ 64 ]. The VEGF xxx family of isoforms, where xxx refers to the number of amino acids within a given isoform, is formed by differential splicing in exons 6 and 7 and the proximal splice site in exon 8 (termed exon 8a).…”

mentioning

confidence: 99%

Molecular Diversity of VEGF-A as a Regulator of Its Biological Activity

et al. 2009

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Age-dependent expression of VEGF isoforms and receptors in the rabbit anterior cruciate ligament

Cited by 14 publications

References 33 publications

Expression of vascular remodelling markers in relation to bradykinin receptors in asthma and COPD

Expression of vascular remodelling markers in relation to bradykinin receptors in asthma and COPD

Use of genetically modified muscle and fat grafts to repair defects in bone and cartilage

Molecular Diversity of VEGF-A as a Regulator of Its Biological Activity

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