1992
DOI: 10.1172/jci115652
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Age-dependent expression of the erythropoietin gene in rat liver and kidneys.

Abstract: Using RNAse protection, we have made quantitative measurements of erythropoietin (EPO) mRNA in liver and kidneys of developing rats (days 1-54), to determine the relative contribution of both organs to the total EPO mRNA, to monitor changes which occur with development, and to compare the hypoxia-induced accumulation of EPO mRNA with the changes in serum EPO concentrations. To determine whether developmental and organ-specific responsiveness is related to the type of hypoxic stimulus, normobaric hypoxia was co… Show more

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Cited by 120 publications
(75 citation statements)
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“…Figure 1 shows the time course of EPO mRNA levels in primary cultures of hepatocytes. Although cells were isolated from normoxic animals EPO mRNA was readily detectable after cell isolation, which is in accordance with previous data from in vivo studies [15]. When cells were preincubated at 20% oxygen for 4 h, as in previous experiments [16] average concentrations of EPO mRNA did not change significantly (not shown), but when preincubation of cells was performed at 40% oxygen, EPO mRNA levels after 4 h were significantly lower than immediately after isolation, and in many experiments became undetectable.…”
Section: Measurement Of Total Rna Synthesis [3h]uridine Incorporationsupporting
confidence: 89%
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“…Figure 1 shows the time course of EPO mRNA levels in primary cultures of hepatocytes. Although cells were isolated from normoxic animals EPO mRNA was readily detectable after cell isolation, which is in accordance with previous data from in vivo studies [15]. When cells were preincubated at 20% oxygen for 4 h, as in previous experiments [16] average concentrations of EPO mRNA did not change significantly (not shown), but when preincubation of cells was performed at 40% oxygen, EPO mRNA levels after 4 h were significantly lower than immediately after isolation, and in many experiments became undetectable.…”
Section: Measurement Of Total Rna Synthesis [3h]uridine Incorporationsupporting
confidence: 89%
“…Hepatocytes have recently been identified as the predominant site of oxygen-dependent EPO production in rats during the early postnatal period [15,32,45], and we have previously observed that the ability of hepatocytes to modulate EPO production in an oxygen-dependent fashion is maintained after isolation of the cells [16]. Extending these observations, studies of the time course of EPO mRNA levels in isolated hepatocytes (Fig.…”
Section: Discussionmentioning
confidence: 52%
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“…The early studies of the liver-to-kidney shift were based on measuring total EPO levels (with bioassays and, subsequently, immunoassays) in response to anaemia in normal-and bilaterally nephrectomised fetal-and new-born sheep [9]. In studies on rats [10], the expression level of EPO mRNA was measured in the respective organs. Measurement of EPO mRNA has shown that the rat liver can contribute with over 33% of the produced EPO during short-term severe hypoxia, and up to 37% after severe haemorrhage [11].…”
Section: Introductionmentioning
confidence: 99%