Age-dependent changes in the response of the lamb ductus arteriosus to oxygen and ibuprofen

Coceani, Flavio; White, E; Bodach, E.; Olley, P. M.

doi:10.1139/y79-126

Cited by 80 publications

(31 citation statements)

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“…The DA of the emu showed a similar increase in contractile response to oxygen, but it occurred earlier in development before internal pipping (Dzialowski and Greyner, 2007). The mammalian DA exhibits a similar increased response to oxygen with gestation and birth (Noel and Cassin, 1976;Coceani et al, 1979). These findings are in contrast to the findings of Å gren, et al (2007) where they observed only a slight increase in contractile strength of the oxygen induced contraction between day 19 and EP embryos.…”

Section: Morphological Changesmentioning

confidence: 57%

Morphological Changes in the Chicken Ductus Arteriosi During Closure at Hatching

Belanger¹,

Copeland²,

Muirhead³

et al. 2008

The Anatomical Record

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The chicken embryo has two functioning ductus arteriosi (DA) during development. These blood vessels connect the pulmonary arteries to the descending aorta providing a right-to-left shunt of blood away from the nonrespiring lungs and to the systemic circuit and chorioallanotic membrane. The DA consists of two distinct tissue types along its length, a muscular proximal portion and an elastic distal portion. During hatching, the DA must close for proper separation of systemic and pulmonary circulation. We examined the morphological changes of the chicken DA before, during, and after hatching. Occlusion of the proximal DA began during external pipping and was complete at hatching. Anatomical remodeling began as early as external pipping with fragmentation of the internal elastic lamina and smooth muscle actin appearing in the neointimal zone. By day 2 posthatch, the proximal DA lumen was fully occluded by endothelial cells and smooth muscle actin positive cells. In contrast, the distal DA was not fully occluded by day 2 posthatch. Increases in Po 2 of the blood serves as the main stimulus for closure of the mammalian DA. The responsiveness of the chicken proximal DA to oxygen increased during hatching, peaking during external pipping. This peak correlated with an increase in blood gas Po 2 and the initial occlusion of the vessel. The distal portion remained unresponsive to oxygen throughout hatching. In conclusion, the chicken DA begins to close during external pipping when arterial Po 2 increases and vessel tone is most sensitive to oxygen.

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Section: Morphological Changesmentioning

confidence: 57%

Morphological Changes in the Chicken Ductus Arteriosi During Closure at Hatching

Belanger¹,

Copeland²,

Muirhead³

et al. 2008

The Anatomical Record

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“…[15][16][17] If treatment with oral ibuprofen is both effective and safe, then it would have the advantages of more widespread availability, simpler administration, and decreased cost. The primary objective of the current study was to determine whether orally administered ibuprofen treatment is efficacious in PDA closure in premature infants with RDS.…”

Section: Discussionmentioning

confidence: 99%

“…Ibuprofen, another prostaglandin synthesis inhibitor, has been shown to be as effective as indomethacin in ductal closure by several investigators who administered it intravenously. [15][16][17] In contrast to indomethacin, ibuprofen does not affect basal cerebral blood flow, 16 -21 cerebral metabolic rate, 18,20 or intestinal or renal hemodynamics. 17,22 If it could be shown to be as efficacious and as safe as the intravenous route, then oral ibuprofen would afford several important advantages: 1) intravenous ibuprofen is not available in the United States or in many other countries, 2) the required oral dose is of minimal volume (0.25-0.5 mL for infants who weigh 500-1000 g), 3) oral administration is extremely simple, and 4) the oral form of the drug is less expensive than the intravenous one.…”

mentioning

confidence: 87%

Closure of Patent Ductus Arteriosus With Oral Ibuprofen Suspension in Premature Newborns: A Pilot Study

et al. 2003

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ABSTRACT. Objective. Patent ductus arteriosus (PDA), a common finding among premature infants, is conventionally treated by intravenous indomethacin. Intravenous ibuprofen was recently shown to be as effective and to have fewer adverse reactions in preterm infants. If equally effective, then oral ibuprofen for PDA closure would have several important advantages over the intravenous route. This study was designed to determine whether oral ibuprofen treatment is efficacious and safe in closure of a PDA in premature infants with respiratory distress syndrome.Methods. Twenty-two preterm newborns (gestational age: 27.5 ؎ 1.75 [range: 23.9 -31 weeks]; weight: 979 ؎ 266 [range: 380-1500 g]) with PDA and respiratory distress syndrome were studied prospectively. They received oral ibuprofen suspension 10 mg/kg/body weight for the first dose, followed at 24-hour intervals by 2 additional doses of 5 mg/kg each, if needed, starting on the second day of life. Echocardiography was performed before treatment and 24 hours after each dose. Every child underwent cranial ultrasonography before and after each ibuprofen dose. The rate of ductal closure, the need for additional treatment, side effects, complications, and the infants' clinical courses were recorded.Results. Ductal closure was achieved in all newborns except for 1 (95.5%), in whom clinically nonsignificant ductal shunting persisted. No infant required surgical ligation of the ductus. There was no reopening of the ductus after closure had been achieved. Fourteen newborns were treated with 1 dose of ibuprofen, 6 were treated with 2 doses, and the remaining 2 were treated with 3 doses. The survival rate at 1 month was 86.4% (19 of 22). Three (13.6%) infants died from the following causes: 1 who was born at 24 weeks' gestation with a birth weight of 380 g died as a result of extreme prematurity complications, necrotizing enterocolitis, and low birth weight; 1 died as a result of Candida sepsis; and the third died as a result of Klebsiella sepsis. Intraventricular hemorrhage was observed in 7 infants. The classification was changed from grade 2 to grade 3 in 1 and from grade 0 to grade 1 or higher in 3 others. The rate of survival to discharge was 86.4% (19 of 22). No bronchopulmonary dysplasia was observed in the study group, and there was no case of tendency to bleed. There were no significant differences in the levels of serum creatinine before and after treatment with oral ibuprofen.Conclusions. Oral ibuprofen suspension may be an effective and safe alternative for PDA closure in premature infants with PDA. However, larger comparative studies are warranted. Pediatrics 2003;112:e354 -e358. URL: http://www.pediatrics.org/cgi/content/full/112/5/ e354; oral ibuprofen, patent ductus arteriosus, premature newborns.ABBREVIATIONS. PDA, patent ductus arteriosus; RDS, respiratory distress syndrome; IVH, intraventricular hemorrhage; NEC, necrotizing enterocolitis.T he incidence of patent ductus arteriosus (PDA) in premature infants who weigh between 500 and 1500 g at birth is appr...

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“…Lamb DA at this liquid scintillation spectrometry. The identification of the band gestational age are capable of only weak constriction without at 220 kD as FN was previously confirmed by Western immuluminal obliteration ( 18). Furthermore.…”

Section: Methodsmentioning

confidence: 66%

Regulation of Fetal Lamb Ductus Arteriosus Smooth Muscle Cell Migration by Indomethacin and Dexamethasone

Koppel

Rabinovitch

1993

Pediatr Res

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ABSTRACT. Postnatal closure of the ductus arteriosus DEX, desamethasone (DA) requires the development, during late gestation, of Ao, aorta "intimal cushions." These structures, which partially oc-PC, prostaglandin clude the vessel lumen, are characterized by smooth muscle FN, fibronectin cell (SMC) migration into an expanded subendothelium. DA SMC migration is dependent upon increased fibronectin (FN) production. We hypothesized that indomethacin (INDO) or dexamethasone, which could influence FN production, may affect SMC migration and DA intimal cushionThe DA is an artery that, during fetal life, serves to divert the formation. SMC harvested from the DA and aorta of 100-flow of blood from the uninflated lungs to the systemic circulad fetal lambs were seeded onto three-dimensional collagen tion. With the initiation of breathing, the lungs are inflated and gels. Migration into the gels was quantitatively assessed the arterial oxygen saturation increases. Through a cytochrome by phase-contrast microscopy. INDO retarded DA SMC P-450 mechanism ( I ) probably linked to endothelin (2, 3), the migration (p < 0.05), but it had no effect on aorta SMC elevated oxygen tension produces a strong muscular contraction migration. Synthesis of FN was measured after ["SI-meoccluding the DA and increasing the flow of blood to the pulthionine radiolabeling of cells, gelatin sepharose extraction monary circulation. Independent of this reversible muscular of conditioned media, and resolution by SDS-PAGE. De-contraction, permanent closure of the DA requires a structural spite the decrease in migration, INDO did not affect FN change. namely, the development of "intimal cushions" (4). synthesis in DA SMC, whereas dexamethasone, a stabi-Failure of the DA to undergo obliteration. a common event in lizer of FN mRNA that increased DA FN synthesis by preterm newborns, allows blood to flow from the Ao into the 44%, had no further effect on SMC migration. We then pulmonary circulation and results in congestive heart failure. investigated whether INDO might be influencing SMC The development of intimal cushions. which occurs independmigration by decreasing collagenase production or altering ently of DA muscular contraction, involves changes in both the cell shape through changes in F-actin polymerization. Col-intima and the media of the vessel with increased deposition of lagenase activity, assessed by zymography using collagen hyaluronan in the subendothelial region associated with migratypes I and IV, was similar in control and treated DA and tion of SMC into this area (5). Studies by Boudreau and Rabiaorta cells. Image analysis of the actin cytoskeleton after novitch (6) revealed that DA SMC harvested from early gestation rhodamine-phalloidin staining of DA SMC, however, re-fetal lambs produced 2-fold more FN than SMC from the Ao. vealed significant shortening of INDO-treated DA SMC Furthermore, the increase in FN production by DA SMC was relative to control, consistent with the observed reduction associated with their enhanced migration rel...

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Age-dependent changes in the response of the lamb ductus arteriosus to oxygen and ibuprofen

Cited by 80 publications

References 0 publications

Morphological Changes in the Chicken Ductus Arteriosi During Closure at Hatching

Morphological Changes in the Chicken Ductus Arteriosi During Closure at Hatching

Closure of Patent Ductus Arteriosus With Oral Ibuprofen Suspension in Premature Newborns: A Pilot Study

Regulation of Fetal Lamb Ductus Arteriosus Smooth Muscle Cell Migration by Indomethacin and Dexamethasone

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