Age‐Dependent Changes in the Distribution of BrdU‐ and TUNEL‐Positive Cells in the Murine Gingival Tissue

Sakai, Takako; Kiyoshima, Tamotsu; Kobayashi, Ieyoshi; Moroi, Ryoji; Ibuki, T.; Nagadome, Mieko; Terada, Yoshihiro; Sakai, Hidetaka

doi:10.1902/jop.1999.70.9.973

Cited by 23 publications

(25 citation statements)

References 18 publications

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“…3). These findings confirm work by Scott et al 19) , Çelenligil-Nazliel et al 4) and Sakai et al 15) . According to Çelenligil-Nazliel et al 4) , the only difference caused by aging is a reduction in conjunctive tissue cell turnover due to increased apoptosis.…”

Section: Discussionsupporting

confidence: 92%

“…A single population was followed up, making this a longitudinal study and minimizing the chances of individual characteristics influencing the results. Many studies have evaluated two experimental times, one in adolescence and the other in senescence 6,15,17,19,24) . This could generate misunderstandings, as observed in this study.…”

Section: Discussionmentioning

confidence: 99%

“…The AgNOR technique has been Aging Effects on Mouse Tongue Epithelium used for over 20 years and, as such, its relationship with cell proliferation rate is well established 2,5,8,[10][11][12]14,18,20,21,23) . Çelenligil-Nazliel et al 4) and Sakai et al 15) used PCNA and BrdU, respectively, which are markers that are directly related to cell proliferation expressed during the interphase. The AgNOR technique has an advantage over other proliferation markers such as Ki-67, PCNA, tritiated thymidine and BrdU, since it provides information about cell proliferation rate, i.e., cell doubling time, whereas the other proliferation markers mentioned indicate whether cells are dividing or not, i.e., the growth fraction 10,11) .…”

Section: Discussionmentioning

confidence: 99%

“…Aging affects many tissues and organs throughout the body 7,16,25) , but there are currently disagreements about certain clinical and histological data concerning its effects on the oral mucosa 6,13,15,19,24) . The major function of the oral mucosa is to protect the deeper tissues and organs in the oral cavity.…”

Section: Introductionmentioning

confidence: 99%

“…Some studies have analyzed the effect of aging on epithelial renewal. Nevertheless, there is no consensus in the literature on how epithelial maturation and proliferation are affected by age 6,13,15,19,24) . Çelenligil-Nazliel et al 4) did not find differences in epithelial renewal.…”

Section: Introductionmentioning

confidence: 99%

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Effects of Aging on Mouse Tongue Epithelium Focusing on Cell Proliferation Rate and Morphological Aspects

Carrard

Pires

Badauy

et al. 2008

Bull. Tokyo Dent. Coll.

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The aim of this study was to investigate cell proliferation rate and certain morphological features of mouse epithelium as aging progresses. Tongue biopsies were performed on female mice (Mus domesticus domesticus) at 2, 8, 14 and 20 months of age as indicative of adolescence, adulthood, early senescence and senescence, respectively. Histological sections of tongue were stained with hematoxylin-eosin and subjected to silver staining for active nucleolar organizer region counting. Cell proliferation rate and epithelial thickness analysis were carried out. Analysis of variance detected no differences between the groups in terms of numbers of silver-stained dots associated with nucleolar proteins. There was an increase in mean epithelial thickness in adult animals, followed by a gradual reduction until senescence. Mean keratin thickness presented an increase at 8 and 20 months of age. This difference is probably related to puberty, growth or dietary habits. Aging has no influence on oral epithelial proliferation rate in mice. A gradual reduction in epithelial thickness is a feature of aging in mammals. A conspicuous increase in the keratin layer was observed in senescence as an adaptative response to the reduction in epithelial thickness. These results suggest that aging affects the oral epithelium maturation process through a mechanism that is not related to cell proliferation.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Effects of Aging on Mouse Tongue Epithelium Focusing on Cell Proliferation Rate and Morphological Aspects

Carrard

Pires

Badauy

et al. 2008

Bull. Tokyo Dent. Coll.

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Comparative Analysis of Gene Expression Patterns for Oral Epithelium-Related Functions with Aging

Ebersole¹,

Orraca²,

Novak³

et al. 2019

Oral Mucosal Immunity and Microbiome

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Age-dependent changes in cell proliferation and cell death in the periodontal tissue and the submandibular gland in mice: a comparison with other tissues and organs

et al. 2007

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This study investigated the age-dependent changes in the number of BrdU- and TUNEL-positive cells in murine gingival tissue and submandibular gland, and compared the findings with those in other tissues and organs. The cell proliferative activity was decreased after 20 weeks of age in epithelial cells of the gingiva, tongue, buccal mucosa and skin. A decreased cell proliferative activity was also associated with aging in the liver and kidney parenchymal cells. Meanwhile, cell death showed peculiar changes in gingival subepithelial tissue, and mucous and serous acini of the submandibular gland. An increase of TUNEL-positive cells was demonstrated in gingival subepithelial tissue after 20-week-old of age. A significant increase of TUNEL-positive cells was also found in the mucous acinar cells in the 20-week-old mice and in the serous acini after 20 weeks. The fluctuation in the number of TUNEL-positive cells in the subepithelial tissue of the skin, and BrdU- and TUNEL-positive staining ratios in the liver was smaller than that in other tissue and organs throughout life. This study may provide useful information for better understanding the influence of aging on the functional alteration that occurs in the gingival tissue and submandibular gland of the elderly.

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Age‐Dependent Changes in the Distribution of BrdU‐ and TUNEL‐Positive Cells in the Murine Gingival Tissue

Cited by 23 publications

References 18 publications

Effects of Aging on Mouse Tongue Epithelium Focusing on Cell Proliferation Rate and Morphological Aspects

Effects of Aging on Mouse Tongue Epithelium Focusing on Cell Proliferation Rate and Morphological Aspects

Comparative Analysis of Gene Expression Patterns for Oral Epithelium-Related Functions with Aging

Age-dependent changes in cell proliferation and cell death in the periodontal tissue and the submandibular gland in mice: a comparison with other tissues and organs

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