Age-Dependent Changes Cooperatively Impact Skeletal Muscle Regeneration after Compartment Syndrome Injury

Zhou, Yu; Lovell, Daniel J.; Bethea, Maigen; Zhan, Wei; Christ, George J.; Söker, Shay; Criswell, Tracy

doi:10.1016/j.ajpath.2014.03.018

Cited by 11 publications

(16 citation statements)

References 32 publications

(49 reference statements)

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“…We have previously shown that the muscles of aged rats contain a significant amount of fibrosis after injury compared with similarly treated muscles from young and adult rats. 33 To determine the effect of MPC transplantation on tissue fibrosis, we quantitated the fibrotic areas represented on Masson's trichrome-stained slides. No change in fibrosis was detected between the muscles compared with uninjured muscles from young and adult animals in any of the cellinjected groups, but an unexpected significant decrease in fibrosis was noted in the PBS-treated muscles (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…Total RNA was isolated from the rat TA muscle using the PerfectPure RNA Fibrous Tissue Kit (5 Prime, Inc., Gaithersburg, MD) as previously described. 33 Quantitative PCR (qPCR) was performed to measure the transcripts of myogenic, fibrogenic, FIG. 1.…”

Section: Mpc Culturementioning

confidence: 99%

“…Injury was induced in rats as previously described. 33,34 In brief, prenatal blood pressure cuffs were secured on the hindlimb proximal to the anterior muscle compartment of anesthetized rats and held at a pressure of 120-140 mmHg for 3 h. Animals were euthanized 14 days after injury for histological analyses. At least three animals were used for each experiment.…”

Section: Mpc Culturementioning

confidence: 99%

“…Contractile function (i.e., torquefrequency relationship) of the left anterior crural muscles was measured in vivo as previously described. 33,34 In brief, the left hindlimb of anesthetized rats was clamped, and the left foot was secured to a custom-made footplate that is attached to the shaft of an Aurora Scientific 305C-LR-FP servomotor, which in turn was controlled using a PC. Electrical stimulus was applied to the left common peroneal nerve.…”

Section: Fig 2 Characterization Of Mpcs (A)mentioning

confidence: 99%

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^{The Impact of Age on Skeletal Muscle Progenitor Cell Survival and Fate After Injury}

Zhou

Lovell

Bethea

et al. 2017

Tissue Engineering Part C: Methods

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Sarcopenia is defined as the loss of skeletal muscle mass and function due to age, and represents a major cause of disability in the elderly population. The contributing factors to the onset of sarcopenia are not well defined, but appear to involve age-dependent changes in both the tissue microenvironment and muscle progenitor cell (MPC) population. MPC transplantation has the potential to be a novel therapy for treatment of muscle dysfunction due to aging or injury, but has not shown significant clinical efficacy to date. The goal of this research was to use a rat model of skeletal muscle injury to examine the differential effects of age on MPC survival, differentiation, and tissue regeneration after transplantation. Fluorescently labeled MPCs, derived from young (YMPCs) and adult (AMPCs) donor rats, were transplanted in the injured tibialis anterior (TA) muscles of young, adult, and aged rats. Our results demonstrated that integration and maturation of YMPCs into mature myofibers were dependent on the age of the host microenvironment; whereas, the integration and maturation of AMPCs were less dependent on age and more dependent on intrinsic cellular changes. These data suggest that the age of both the host microenvironment and cells for transplantation must be considered when designing cell therapy regimens.

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Section: Resultsmentioning

confidence: 99%

Section: Mpc Culturementioning

confidence: 99%

Section: Mpc Culturementioning

confidence: 99%

Section: Fig 2 Characterization Of Mpcs (A)mentioning

confidence: 99%

See 2 more Smart Citations

^{The Impact of Age on Skeletal Muscle Progenitor Cell Survival and Fate After Injury}

Zhou

Lovell

Bethea

et al. 2017

Tissue Engineering Part C: Methods

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“…The underlying molecular mechanisms of I/R injury are fairly well understood, and a number of protective agents and cell therapies have been tested as potential treatments. In considering therapies, however, patient characteristics, including age and activity level, may dictate the severity and rate of recovery after I/R injury . Surprisingly, the benefits of physical activity in protecting or improving recovery after limb I/R injury have not been tested previously in an animal model.…”

mentioning

confidence: 99%

Activity attenuates skeletal muscle fiber damage after ischemia and reperfusion

2015

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Introduction: In this investigation we aimed to determine whether: (1) physical activity protects rat skeletal muscle from ischemia/reperfusion (I/R) injury; and (2) continued activity after I/R improves the rate of healing. Methods: Rats were divided into sedentary or active (voluntary wheel running) groups. Active rats ran for 4 weeks before I/R or 4 weeks before plus 4 weeks after I/R. Results: Activity before I/R resulted in 73.2% less muscle damage (Evans blue dye inclusion). Sedentary and active rats had a similar decline in neural‐evoked (∼99%) and directly stimulated (∼70%) in vivo muscle torque, and a similar reduction in junctophilin 1. Active rats produced 19% and 15% greater neural‐evoked torque compared with sedentary rats at 14 and 28 days postinjury, respectively, although the rate of recovery appeared similar. Conclusions: Activity protects against long‐term muscle damage, but not short‐term neural injury or excitation‐contraction uncoupling. Continued activity neither accelerates nor hinders the rate of functional recovery. Muscle Nerve 52: 640–648, 2015

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Diagnostic Modalities for Acute Compartment Syndrome of the Extremities

et al. 2019

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IMPORTANCE Acute compartment syndrome (ACS) can cause catastrophic tissue damage leading to permanent muscle and nerve loss. Acute compartment syndrome is a clinical diagnosis, with intracompartmental pressure (ICP) used in equivocal cases. There are no reliable diagnostic methods. The clinical evaluation is impossible to standardize, and the threshold for ICP has been known to be unreliable; thus, guidelines for diagnosis can result in overtreatment or delayed diagnosis.OBJECTIVE To present and review the advantages and disadvantages of each diagnostic modality and identify gaps that need to be addressed in the future and to review the most used and appropriate animal and human ACS models. EVIDENCE REVIEWWe included clinical studies and animal models investigating diagnostic modalities for ACS of the extremities. A MEDLINE and Web of Science search was performed. The protocol for the study was registered on PROSPERO (CRD42017079266). We assessed the quality of the clinical studies with Newcastle-Ottawa scale and reported level of evidence for each article.FINDINGS Fifty-one articles were included in this study, reporting on 38 noninvasive and 35 invasive modalities. Near-infrared spectroscopy and direct ICP measurement using a Stryker device were the most common, respectively. Cadaveric studies used saline infusions to create an ACS model. Most studies with human participants included injured patients with acquired ACS or at risk of developing ACS. In healthy human participants, tourniquets formed the most commonly used ACS model. Application of tourniquets and infusion of saline or albumin were the most used ACS models among animal studies. CONCLUSIONS AND RELEVANCEThis article reports on the most common as well as many new and modified diagnostic modalities, which can serve as inspiration for future investigations to develop more effective and efficient diagnostic techniques for ACS. Future studies on diagnostic modalities should include the development of tools for continuous assessment of ICP to better identify the earliest alterations suggestive of impending ACS. With the advent of such technologies, it may be possible to develop far less aggressive and more effective approaches for early detection of ACS.

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Age-Dependent Changes Cooperatively Impact Skeletal Muscle Regeneration after Compartment Syndrome Injury

Cited by 11 publications

References 32 publications

^{The Impact of Age on Skeletal Muscle Progenitor Cell Survival and Fate After Injury}

^{The Impact of Age on Skeletal Muscle Progenitor Cell Survival and Fate After Injury}

Activity attenuates skeletal muscle fiber damage after ischemia and reperfusion

Diagnostic Modalities for Acute Compartment Syndrome of the Extremities

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