Age-dependent alterations in hepatic subcellular copper distribution and plasma ceruloplasmin

Evans, Gerald W.; Myron,; Cornatzer, NF; We, Cornatzer

doi:10.1152/ajplegacy.1970.218.1.298

Cited by 60 publications

(18 citation statements)

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“…Increased Cp activity of rat plasma during the suckling period has been observed previously (Evans et al, 1970b). Our data confirm that process and extend it to another rodent model, the mouse.…”

Section: Discussionsupporting

confidence: 91%

Plasma peptidylglycine alpha-amidating monooxygenase (PAM) and ceruloplasmin are affected by age and copper status in rats and mice

Prohaska

Broderius

2006

Comparative Biochemistry and Physiology Part B: Biochemistry an

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In an attempt to identify a sensitive and improved marker of mammalian copper status during neonatal development experiments compared two plasma cuproenzymes, peptidylglycine α-amidating monooxygenase (PAM ), an enzyme involved in peptide posttranslational activation, to ceruloplasmin (Cp), a ferroxidase involved in iron mobilization. Dietary Cu deficiency (Cu−) was studied in dams and offspring at postnatal age 3 (P3), P12, and P28. Rodent Cp activity rose during lactation whereas PAM activity fell. Reduction in Cp activity was more severe than reduction in PAM activity in Cu− offspring and dams. Cp activity was greater in rats than mice whereas PAM activity was similar in adults but greater in mouse than rat pups. Both cuproenzymes changed during neonatal development and when dietary copper was limiting. With proper controls, each enzyme can be used to assess copper status.

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Section: Discussionsupporting

confidence: 91%

Plasma peptidylglycine alpha-amidating monooxygenase (PAM) and ceruloplasmin are affected by age and copper status in rats and mice

Prohaska

Broderius

2006

Comparative Biochemistry and Physiology Part B: Biochemistry an

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“…Several studies indicated that CP activity increases during the early postnatal develop mental period o f rats [22,23], pigs [24] and that o f humans [25]. Our results show the same 3-fold increase in the activity as it was measured in rat blood by Evans and Wieder-anders [22].…”

Section: Discussionsupporting

confidence: 86%

Effects of Age and Turpentine-Induced Inflammation on the Activity of Ceruloplasmin from Blood of CFY Rats

Semsei¹

1991

Gerontology

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The activity of ceruloplasmin (CP) increased 3-fold in rat blood between 1 week and 20 months of age, but the difference in CP activity of adult and old rats was only 14%, which still is statistically significant (p < 0.01). The increase in NaCl and KCl concentrations in vitro resulted in the same exponential decrease in activities of CP from both young and old animals. Turpentine-induced inflammation caused an increase in blood CP levels in both young (52%) and old (25%) animals compared to age-matched controls (p < 0.001 and p < 0.01, respectively). These results indicate that the antioxidant activity of CP is maintained as a function of age in rat blood, which is beneficial for preventing the increase in lipid peroxidation.

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“…Several LEC and LEA rats aged 2 d, 1,4,8,12,16,20,24,28, and 32 wk, and 13 mo were anesthetized by ether, and blood was taken by heart puncture. Saline-perfused liver, kidney, small intestine, and brain were taken and immediately frozen at -70°C.…”

Section: Animalsmentioning

confidence: 99%

“…They were suspended in the same solution and homogenized in a Potter-Elvehjem homogenizer with a Teflon pestle. The procedure for subcellular fractionation was carried out as described by Evans et al (20). The pellet, after being centrifuged for 10 min at 14 000 x g, containing both mitochondria and lysosomes, was referred to as the large-granule fraction.…”

Section: Animalsmentioning

confidence: 99%

Inherited Copper Toxicity in Long-Evans Cinnamon Rats Exhibiting Spontaneous Hepatitis: A Model of Wilson's Disease

et al. 1992

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Age-dependent alterations in hepatic subcellular copper distribution and plasma ceruloplasmin

Cited by 60 publications

References 0 publications

Plasma peptidylglycine alpha-amidating monooxygenase (PAM) and ceruloplasmin are affected by age and copper status in rats and mice

Plasma peptidylglycine alpha-amidating monooxygenase (PAM) and ceruloplasmin are affected by age and copper status in rats and mice

Effects of Age and Turpentine-Induced Inflammation on the Activity of Ceruloplasmin from Blood of CFY Rats

Inherited Copper Toxicity in Long-Evans Cinnamon Rats Exhibiting Spontaneous Hepatitis: A Model of Wilson's Disease

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