Age dependence of tissue plasminogen activator concentrations in plasma, as studied by an improved enzyme-linked immunosorbent assay.

Rånby, Mats; Bergsdorf, Nils; Nilsson, Tobias; Mellbring, G; Winblad, Bengt; Bucht, Gösta

doi:10.1093/clinchem/32.12.2160

Cited by 200 publications

(39 citation statements)

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“…Plasma tissue plasminogen activator (tPA) antigen concentrations were determined in citrated plasma by an enzyme-linked immunosorbent assay (Imulyse. Biopool AB, UmeA, Sweden) [5]. Plasma plasminogen activator inhibitor (PAI) activity was measured in citrated plasma with a chromogen substrate assay (Spectrolyse/pL, Biopool AB, Umel, Sweden) [6].…”

Section: Assay Methodsmentioning

confidence: 99%

Cardiovascular risk factors in young snuff‐users and cigarette smokers

Eliasson¹,

Lundblad²,

Hägg

1991

Journal of Internal Medicine

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We studied cardiovascular risk factors in 21 young men who were habitual snuff-users, and compared them with the same risk factors in 18 non-tobacco-users and 19 cigarette smokers of the same age and body mass index. Both snuff-users and smokers showed increased levels of alcohol and coffee consumption and a decreased level of physical exercise compared to non-users. Both groups of tobacco-users showed increased serum insulin levels compared to the control group at similar blood glucose concentrations. In contrast to the smokers, snuff-users showed no significant elevation of diastolic blood pressure, haemoglobin concentrations, white cell count, serum cholesterol or triglyceride levels. Snuff users had higher plasma fibrinogen levels than non-users (P = 0.07). The use of snuff by young men appears to have less impact than smoking on cardiovascular risk factors, with the possible exception of elevated serum insulin and plasma fibrinogen levels.

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Section: Assay Methodsmentioning

confidence: 99%

Cardiovascular risk factors in young snuff‐users and cigarette smokers

Eliasson¹,

Lundblad²,

Hägg

1991

Journal of Internal Medicine

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“…Enzyme-linked immunosorbent assays were used for measurements of; t-PA antigen using Imulyse ™ t-PA (Biopool AB.) [16], PAI-1 antigen using Imulyse ™ PAI-1 (Biopool AB.) [17];  -dimers using TintElize ®  -dimer (Biopool AB.)…”

Section: Blood Sampling and Analysesmentioning

confidence: 99%

Endothelial dysfunction in Buerger's disease and its relation to markers of inflammation

Joras

Poredoš

Fras

2006

Eur J Clin Investigation

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“…EC-SOD in plasma was analysed by an enzyme-linked immunosorbent assay [28], as were the concentrations of tissue plasminogen activator, tPA [29]. tPA activity was determined with a parabolic rate assay based on fibrin stimulation of the tPA-mediated conversion of plasminogen and plasmin using the Spectrolyse/fibrin kit from Biopool AB (Umeå, Sweden) [27,30].…”

Section: Biochemical Analysesmentioning

confidence: 99%

Two variants of extracellular‐superoxide dismutase: relationship to cardiovascular risk factors in an unselected middle‐aged population

Nilsson

Israelsson

et al. 1997

Journal of Internal Medicine

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Abstract. Marklund SL, Nilsson P, Israelsson K, Schampi I, Peltonen M, Asplund K (University Hospital, Umeå, Sweden). Two variants of extracellular-superoxide dismutase: relationship to cardiovascular risk factors in an unselected middle-aged population. J Intern Med 1997; 242: 5-14.Objective. Description of the distribution of plasma levels of two variants of extracellular-superoxide dismutase (EC-SOD) and their relationship to cardiovascular risk factors in the general population. Design. A cross-sectional study. Setting. Norrbotten and Västerbotten counties in northern Sweden. Subjects. Four thousand, nine hundred 25-74-yearold randomly selected subjects. Main outcome measures. Plasma levels of EC-SOD.Genotyping by an allele-specific PCR method. Results. Plasma EC-SOD levels showed a distinct bimodal distribution with the smaller group (3.8%) having a variant of the enzyme with about eight-fold-higher plasma levels. Genotyping was performed in 65 individuals with the high-level variant. All but one were found to carry the same mutation, Arg213Gly, affecting the heparin-binding domain of EC-SOD. Subjects with the high-level variant of EC-SOD had modestly higher body mass index and higher levels of serum cholesterol, serum triglycerides and plasma fibrinogen than those with the common EC-SOD phenotype. Within the population with common EC-SOD, the plasma levels were lower in men than in women and increased with age. Low levels of common phenotype EC-SOD were associated with smoking, high plasma levels of fibrinogen and low activity of tissue plasminogen activator in both univariate and multivariate analyses. Obesity and total serum cholesterol were associated with high common phenotype EC-SOD levels. Conclusions. A high-level variant of EC-SOD caused by one and the same mutation and with low tissue binding and high plasma levels is present in approximately four per cent of an unselected middle-aged population in northern Sweden. Plasma levels of EC-SOD may be modulated by lifestyle factors such as smoking and show a complex covariation with many of the conventional cardiovascular risk factors.

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Age dependence of tissue plasminogen activator concentrations in plasma, as studied by an improved enzyme-linked immunosorbent assay.

Cited by 200 publications

References 0 publications

Cardiovascular risk factors in young snuff‐users and cigarette smokers

Cardiovascular risk factors in young snuff‐users and cigarette smokers

Endothelial dysfunction in Buerger's disease and its relation to markers of inflammation

Two variants of extracellular‐superoxide dismutase: relationship to cardiovascular risk factors in an unselected middle‐aged population

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