Age changes in the number and proliferation of fibroblasts in the human skin

Gunin, G.; Kornilova, N. K.; Petrov, Vadim V.; Vasilyeva, O. V.

doi:10.1134/s2079057011040059

Cited by 24 publications

(24 citation statements)

References 21 publications

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“…Cell proliferation, MMP release and collagen deposition results ( Fig. 4a-d) corroborated in vivo observations described in the literature, that is, progressive decrease over time of fibroblasts proliferation [28,29] and collagen dermal content [30] and increase in MMPs with global ageing [31,32]. MMPs concentrations in culture supernatants were higher at t2 h than at t6 h and after 24 h under menopausal conditions compared with non-menopausal conditions, suggesting a rapid but not stable increase linked with fresh medium addition.…”

Section: In Vitro Simulation Of Menopause (Step 4)supporting

confidence: 90%

Development of an in vitro model of menopause using primary human dermal fibroblasts

Remoué¹,

Molinari²,

Andrès³

et al. 2013

Intern J of Cosmetic Sci

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Our results demonstrated that the increased procollagen synthesis with menopausal conditions was not sufficient to compensate for the MMPs' catabolic effects and/or the impaired procollagen protein maturation, resulting in a decrease in extracellular collagen content. These findings add to the overall understanding of hormone-dependent skin behaviour and highlight the suitability of this in vitro model for cosmetic actives testing aiming to underpin claims of anti-ageing efficacy, specifically for menopausal women, regarding collagen metabolism and balance of types, for maintenance of dermal mechanical properties.

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Section: In Vitro Simulation Of Menopause (Step 4)supporting

confidence: 90%

Development of an in vitro model of menopause using primary human dermal fibroblasts

Remoué¹,

Molinari²,

Andrès³

et al. 2013

Intern J of Cosmetic Sci

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“…That is, the older the patient at the time of ADM implantation, the more likely that the original ADM was near-completely degraded. While other studies looking at the impact of age on scaffold degradation and ADM remodeling are lacking, a reduction in cellular proliferation, and in particular fibroblasts, 33 have been shown to be age-related and clinically, advanced age is a risk factor for ADM explantation when used in prosthetic breast reconstruction. 34 Not surprisingly, our data suggest that a history of smoking is associated with less extensive bioabsorption and constructive remodeling of the ADM.…”

Section: Discussionmentioning

confidence: 99%

Remodeling Characteristics and Collagen Distributions of Biologic Scaffold Materials Biopsied From Postmastectomy Breast Reconstruction Sites

Cavallo

Gangopadhyay

Dudas

et al. 2015

Annals of Plastic Surgery

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OBJECTIVE The study purpose was to evaluate the associations between patient characteristics and the histologic remodeling scores of acellular dermal matrices (ADMs) biopsied from breast reconstruction sites in the first attempt to generate a multivariable risk prediction model of non-constructive remodeling. It was hypothesized that host characteristics and surgical site assessments predict the degree of graft remodeling for ADMs used during breast reconstruction. METHODS ADMs were biopsied from the breast reconstruction sites of n=62 patients during a subsequent breast procedure, stained with Hematoxylin and Eosin, and evaluated according to a semi-quantitative scoring system for remodeling characteristics [cell types (CT), cell infiltration (CI), extracellular matrix (ECM) deposition, scaffold degradation (SD), fibrous encapsulation (FE), and neovascularization (NEO)] and a mean composite score (CR). Biopsies were stained with Sirius Red and Fast Green, and analyzed to determine the collagen I:III ratio. Based on univariate analyses between subject clinical characteristics and the histologic remodeling scores, cohort variables were selected for multivariable regression models using a p ≤0.20. RESULTS The CR score model yielded 3 variables: pack-year history, corticosteroid use, and radiation timing (r2pseudo=0.81). The model for collagen I yielded 2 variables: corticosteroid use and reason for reoperation (r2pseudo=0.78). The model for collagen III yielded 1 variable: reason for reoperation (r2pseudo=0.35). CONCLUSION These preliminary results constitute the first steps in generating a risk prediction model that predicts the patients and clinical circumstances most likely to experience non-constructive remodeling of biologic grafts used to reconstruct the breast.

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“…In addition, the amount of GAGs, the water-holding fi lling material between the collagen bundles, decreases with age (Ghersetich et al 1994 ;Bernstein et al 1996b ;Fleischmajer et al 1972 ), explaining the reduced hydration of the skin and possibly the impairment of cell movement and nutrition. All this correlates with a reduced number of dermal fi broblasts (Gunin et al 2011a ;West 1994 ) and mast cells (West 1994 ). At the age of 80 years, the loss of elastic fi bers is as high as 50 %, which is mainly due to the progressive thinning of the skin and results in dramatic loss of elastic properties (Frances et al 1990 ).…”

Section: Intrinsically Aged Skinmentioning

confidence: 99%