Age changes in the crystallinity of bone mineral and in the disorder of its crystal

Matsushima, Norio; Hikichi, Kunio

doi:10.1016/0304-4165(89)90004-4

Cited by 32 publications

(26 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Despite all the criticism of hydrazine deproteination, it is still widely used in bone mineral research [19][20][21][22][23][24] and there are a great number of researchers who claim that it either causes only insignificant alterations to bone mineral [23][24][25][26] or does not alter bone mineral in any way [12,27].…”

Section: Co 2àmentioning

confidence: 99%

Effect of hydrazine deproteination on bone mineral phase: A critical view

Bertazzo

Bertran

2008

Journal of Inorganic Biochemistry

View full text Add to dashboard Cite

Section: Co 2àmentioning

confidence: 99%

Effect of hydrazine deproteination on bone mineral phase: A critical view

Bertazzo

Bertran

2008

Journal of Inorganic Biochemistry

View full text Add to dashboard Cite

“…S1). Nine 2×2×10 mm 3 blocks were cut in the longitudinal direction and subsequently dehydrated (48 hours in ethanol 70 % and 100 %) and slowly dried in a desiccator. One block was used as a control, while the others were heated to eight temperatures: 100, 200, 300, 400, 600, 700, 800 and 1000 °C for 10 min in vacuum (10 -2 mbar) inside quartz tube and cooled in air.…”

Section: Methodsmentioning

confidence: 99%

“…Bone tissue is a biological nanocomposite material essentially composed of hydrated collagen fibrils of ~100 nm in diameter and up to several microns in length, reinforced by platelet-shaped nanocrystals of calcium phosphate apatite of ~ 4×25×50 nm 3 in size [1]. These mineralized fibrils constitute the building blocks of bone tissue, and their specific arrangement is known to depend primarily on the dynamics of the formation and repair processes.…”

Section: Introductionmentioning

confidence: 99%

“…Nevertheless, as a natural extension of bone mineral density (BMD) analysis, an important marker in current clinical studies, the following key characteristics of the mineral nanocrystals have been identified as potential markers of age and diseases: chemical composition, crystallinity vs disorder, crystal structure, size, shape and orientation [3][4]. Recent progress in the field showed that in order to obtain a deeper medical insight into the mechanisms of bone function, several such parameters need to be combined and correlated to properties at larger length scales [5].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Ultrafine heat-induced structural perturbations of bone mineral at the individual nanocrystal level

et al. 2018

View full text Add to dashboard Cite

To cite this version:M. Verezhak, E.F. Rauch, M. Veron, C. Lancelon-Pin, J.-L. Putaux, et al.. Ultrafine heat-induced structural perturbations of bone mineral at the individual nanocrystal level. Acta Biomaterialia, Elsevier, 2018Elsevier, , 10.1016Elsevier, /j.actbio.2018 Online version: https://www.sciencedirect.com/science/article/pii/S1742706118301946 Abstract: The nanoscale characteristics of the mineral phase in bone tissue such as nanocrystal size, organization, structure and composition have been identified as potential markers of bone quality. However, such characterization remains challenging since it requires combining structural analysis and imaging modalities with nanoscale precision. In this paper, we report the first application of automated crystal orientation mapping using transmission electron microscopy (ACOM-TEM) to the structural analysis of bone mineral at the individual nanocrystal level. By controlling the nanocrystal growth of a cortical bovine bone model artificially heated up to 1000 ºC, we highlight the potential of this technique. We thus show that the combination of sample mapping by scanning and the crystallographic information derived from the collected electron diffraction patterns provides a more rigorous analysis of the mineral nanostructure than standard TEM. In particular, we demonstrate that nanocrystal orientation maps provide valuable information for dimensional analysis. Furthermore, we show that ACOM-TEM has sufficient sensitivity to distinguish between phases with close crystal structures and we address unresolved questions regarding the existence of a hexagonal to monoclinic phase transition induced by heating. This first study therefore opens new perspectives in bone characterization at the nanoscale, a daunting challenge in the biomedical and archaeological fields, which could also prove particularly useful to study the mineral characteristics of tissue grown at the interface with biomaterials implants.

show abstract

“…However, other factors also play a role: macroarchitecture of bones [2], cortical thickness [3], quality of bone crystal and of collagen network [4] and trabecular microarchitecture. The last plays a major role [5], and is a part of the definition of osteoporosis: 'disease characterized by low bone mass and microarchitectural deterioration of bone tissue, leading to enhanced bone fragility and consequent increase in fracture risk' [6].…”

Section: Introductionmentioning

confidence: 99%

Fractal Analysis of Trabecular Bone Texture on Radiographs: Discriminant Value in Postmenopausal Osteoporosis

Pothuaud¹,

Lespessailles²,

Harba

et al. 1998

Osteoporosis International

147

View full text Add to dashboard Cite

Trabecular bone microarchitecture cannot be routinely evaluated. We have developed and validated a fractal analysis of trabecular bone texture on calcaneus radiographs. The aim of this work was to evaluate the ability of the fractal analysis to discriminate a group of 39 postmenopausal women with osteoporotic (OP) vertebral crush fractures (68.0 +/- 10.8 years) from an age-matched control group of 39 women (68.0 +/- 10.7 years). The value of the fractal analysis was compared with the value of the femoral neck bone mineral density (FNBMD) and trochanteric bone mineral density (TRBMD). The result is expressed by the parameter Hmean (Hmean = 2 - fractal dimension). Hmean value was 0.691 +/- 0.050 in the OP group versus 0.739 +/- 0.024 in the controls, while FNBMD was 0.598 +/- 0.113 g/cm2 versus 0.645 +/- 0.109 g/cm2 and TRBMD was 0.512 +/- 0.108 g/cm2 versus 0.594 +/- 0.106 g/cm2 respectively. The statistical significance of the Hmean test (p < 0.0001) was higher than for FNBMD (p < 0.05) and for TRBMD (p = 0.0004). We used a receiver operating characteristic (ROC) curve to check this superiority. The area under the ROC curve was 0.824 for Hmean, 0.633 for FNBMD and 0.727 for TRBMD. This superiority of the Hmean ROC curve was statistically significant versus FNBMD, but not versus TRBMD. In a second analysis, we studied the subgroups of OP patients and controls with overlapping FNBMD or TRBMD values to check whether the fractal dimension test could be discriminant in these subgroups. Significant statistical differences were found for Hmean between OP patients and controls in the overlapping subgroup for FNBMD or TRBMD (respectively p = 0.006 and p < 0.02). These data confirm that the fractal analysis of texture on calcaneus radiographs is able to discriminate OP patients with vertebral crush fracture from controls. This discrimination was stronger than that obtained by FNBMD or TRBMD alone. It was also present when we compared subgroups with overlapping values of FNBMD or TRBMD.

show abstract

Age changes in the crystallinity of bone mineral and in the disorder of its crystal

Cited by 32 publications

References 18 publications

Effect of hydrazine deproteination on bone mineral phase: A critical view

Effect of hydrazine deproteination on bone mineral phase: A critical view

Ultrafine heat-induced structural perturbations of bone mineral at the individual nanocrystal level

Fractal Analysis of Trabecular Bone Texture on Radiographs: Discriminant Value in Postmenopausal Osteoporosis

Contact Info

Product

Resources

About