Age-associated polyamines in peripheral blood cells and plasma in 20 to 70 years of age subjects

Abstract

Dietary polyamines have been associated with slowing ageing processes and various pathologies, raising the importance of establishing reference values at different ages throughout life. This study aimed to analyse age-dependent variations in polyamine content using peripheral blood cells and plasma in a healthy and homogeneous population. Peripheral blood of 193 volunteers of both sexes (20–70 years), selected by convenience, was processed to separate cells and plasma. A pre-column derivatization method was used to determine the amines by HPLC (nmol or pmol/mg protein or nmol/ml) to analyse their association with the age (continuous or ordinal in decades) of the subjects. Putrescine and spermine weakly declined significantly in mononuclear cells with age. In erythrocytes and plasma, putrescine showed an evident decrease in the 60–70-year-old group compared to the rest. The ratios between polyamines, mainly in erythrocytes, decreased in the 60–70 years age group and increased the ratio of putrescine in mononuclear cells/erythrocytes. The ratio of putrescine in mononuclear cells/erythrocytes was higher in the 60–70-year-old age group than in the rest. In a sample of subjects (20–29 vs. 60–70 years), whole blood polyamines were not significantly different when differences existed in erythrocytes. Polyamine homeostasis in blood cells and plasma changed with age. Putrescine declined in mononuclear cells and decreased in erythrocytes and plasma in the decade of the 60 s. Further studies should establish an age-dependent phenotype and whether polyamines’ supplementation could restore the decreased values and be associated with long-term overall biological benefits.

The Molecular Role of Polyamines in Age-Related Diseases: An Update

“…Furthermore, elevated polyamine levels are also linked to age-related diseases such as PD and AD [67,100]. This contrasts with the conventional perspective, which primarily attributes the decline in polyamine homeostasis to the aging process [101]. This paradox highlights the challenging nature of categorizing the dynamics of polyamines in age-related diseases and their potential use as therapeutic adjuvants.…”