Age-associated modifications of Base Excision Repair activities in human skin fibroblast extracts

Pons, Bénédicte; Belmont, Anne-Sophie; Masson-Genteuil, Gwénaëlle; Chapuis, Violaine; Oddos, Thierry; Sauvaigo, Sylvie

doi:10.1016/j.mad.2010.09.002

Cited by 25 publications

(25 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We have recently shown that there is a general decrease in DNA repair capacity in aging dermal fibroblasts. Indeed, using two different types of DNA repair measurement that directly measure the activity on human dermal fibroblasts nuclear extracts on plasmid [58] and oligonucleotides [59,60] bearing specific damages, we showed that the level of NER and BER are dramatically reduced in dermal fibroblasts from a group of female volunteers with age comprised between 40 and 50 years old compared to a results obtained in a younger group 20-30 years old for both chronically UV-exposed skin or non-exposed skin site [61,62]. Sauvaigo et al also demonstrat-ed that SSB repair decreased with aging in dermal fibroblasts [60].…”

Section: Nf-κb and The Decrease In Dna Repair Capacity Of Dermal Fibrmentioning

confidence: 90%

Regulation of DNA Repair Process by the Pro-Inflammatory NF-κB Pathway

Kaur¹,

Oddos²,

Tucker-Samaras³

et al. 2013

New Research Directions in DNA Repair

Self Cite

View full text Add to dashboard Cite

Section: Nf-κb and The Decrease In Dna Repair Capacity Of Dermal Fibrmentioning

confidence: 90%

Regulation of DNA Repair Process by the Pro-Inflammatory NF-κB Pathway

Kaur¹,

Oddos²,

Tucker-Samaras³

et al. 2013

New Research Directions in DNA Repair

Self Cite

View full text Add to dashboard Cite

“…Cell nuclear extracts were prepared as already described [32], [36]. Typical protein content was 1 mg/mL.…”

Section: Methodsmentioning

confidence: 99%

“…The relevance and advantages of this concept have been demonstrated in aging studies and in an investigation of the consequences of sun photoexposure using human skin cell samples [32]–[34]. We assessed the DNA repair signatures of a panel of 7 cancer cell lines derived from 4 tumour types, treated by 5 cytotoxic anticancer drugs.…”

Section: Introductionmentioning

confidence: 99%

Functional DNA Repair Signature of Cancer Cell Lines Exposed to a Set of Cytotoxic Anticancer Drugs Using a Multiplexed Enzymatic Repair Assay on Biochip

et al. 2012

Self Cite

View full text Add to dashboard Cite

The development of resistances to conventional anticancer drugs compromises the efficacy of cancer treatments. In the case of DNA-targeting chemotherapeutic agents, cancer cells may display tolerance to the drug-induced DNA lesions and/or enhanced DNA repair. However, the role of DNA damage response (DDR) and DNA repair in this chemoresistance has yet to be defined. To provide insights in this challenging area, we analyzed the DNA repair signature of 7 cancer cell lines treated by 5 cytotoxic drugs using a recently developed multiplexed functional DNA repair assay. This comprehensive approach considered the complexity and redundancy of the different DNA repair pathways. Data was analyzed using clustering methods and statistical tests. This DNA repair profiling method defined relevant groups based on similarities between different drugs, thus providing information relating to their dominant mechanism of action at the DNA level. Similarly, similarities between different cell lines presumably identified identical functional DDR despite a high level of genetic heterogeneity between cell lines. Our strategy has shed new light on the contribution of specific repair sub-pathways to drug-induced cytotoxicity. Although further molecular characterisations are needed to fully unravel the mechanisms underlying our findings, our approach proved to be very promising to interrogate the complexity of the DNA repair response. Indeed, it could be used to predict the efficacy of a given drug and the chemosensitivity of individual patients, and thus to choose the right treatment for individualised cancer care.

show abstract

“…The excision reactions were conducted using the modified oligonucleotide arrays already described elsewhere [20,21]. The principle of these oligonucleotide arrays and oligonucleotides characteristics were detailed in a previous study [20].…”

Section: Dna Repair Assaysmentioning

confidence: 99%

“…A more comprehensive assay allowing investigation of BER has been developed, ensuring simultaneous measurement of several excision activities [18][19][20][21][22]. This new approach was made possible by the use of microarrays functionalized by fluorescent lesionbearing oligonucleotides.…”

Section: Introductionmentioning

confidence: 99%

A comprehensive approach to determining BER capacities and their change with aging in Drosophila melanogaster mitochondria by oligonucleotide microarray

et al. 2014

Self Cite

View full text Add to dashboard Cite

DNA repair mechanisms are key components for the maintenance of the essential mitochondrial genome. Among them, base excision repair (BER) processes, dedicated in part to oxidative DNA damage, are individually well known in mitochondria. However, no large view of these systems in differential physiological conditions is available yet. Combining the use of pure mitochondrial fractions and a multiplexed oligonucleotide cleavage assay on a microarray, we demonstrated that a large range of glycosylase activities were present in Drosophila mitochondria. Most of them were quantitatively different from their nuclear counterpart. Moreover, these activities were modified during aging.

show abstract

Age-associated modifications of Base Excision Repair activities in human skin fibroblast extracts

Cited by 25 publications

References 25 publications

Regulation of DNA Repair Process by the Pro-Inflammatory NF-κB Pathway

Regulation of DNA Repair Process by the Pro-Inflammatory NF-κB Pathway

Functional DNA Repair Signature of Cancer Cell Lines Exposed to a Set of Cytotoxic Anticancer Drugs Using a Multiplexed Enzymatic Repair Assay on Biochip

A comprehensive approach to determining BER capacities and their change with aging in Drosophila melanogaster mitochondria by oligonucleotide microarray

Contact Info

Product

Resources

About