Objective: In this study, it was aimed to evaluate the relationship between chronological ages and pulp areas of mandibular canine teeth of patients that received and not received orthodontic treatment. Methods: 102 patients that completed fixed orthodontic treatment and between the ages of 13-24, and 102 age and sex-matched control group subjects were included in the study. A total of 204 dental panoramic radiographs taken with the same procedures and with the same device (Soredex; Cranex Novus, Tuusula, Finland) were evaluated in this study. The pulp areas of the mandibular canine teeth were measured using the Image J software (US National Institutes of Health, Bethesda, MD). Data were analyzed statistically. Results: In both the orthodontic group (r =-0,511) and the control group (r =-0,592), there was a negative correlation between chronological age and pulp area. There was no significant difference between the groups in terms of the pulp area and gender (p >0.05). Conclusion: Orthodontic treatment did not result in a significant difference in the correlation between the pulp area and the chronological age.