Age and Visual Experience-dependent Expression of NMDAR1 Splice Variants in Rat Retina

Manta, Georgia A.; Spathis, Athanasios; Taraviras, Stavros; Kouvelas, Elias D.; Mitsacos, Ada

doi:10.1007/s11064-011-0467-5

Cited by 5 publications

(1 citation statement)

References 69 publications

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“…The relative amount of gene expression was calculated using the 2 −ΔΔCt method. 30,31) Immunocytochemical Analysis The neurons on coatedglass chamber slide were treated with 3, 10, and 30 µM nobiletin, or with 0.1% DMSO, for 24 h, followed by washing with DPBS, fixation with 4% paraformaldehyde, and permeabilization with 0.2% Triton X-100. They were then blocked with 3% BSA/DPBS for 1 h at room temperature.…”

Section: Methodsmentioning

confidence: 99%

Nobiletin Reduces Intracellular and Extracellular β-Amyloid in iPS Cell-Derived Alzheimer’s Disease Model Neurons

Kimura

Shimizu

Koyama

et al. 2018

Biological & Pharmaceutical Bulletin

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Alzheimer's disease (AD) is the most common cause of dementia, with progressive memory impairment. Recently, neprilysin, a β-amyloid (Aβ)-degrading enzyme has become featured as a drug target for AD. Previously, we identified nobiletin from citrus peels as a natural compound possessing anti-dementia activity. In addition, we demonstrated that nobiletin improved memory in memory-impaired animals and, further, that Aβ levels were markedly decreased in the brains of these animals. We demonstrated in vitro that nobiletin up-regulates neprilysin expression and activity in human neuroblastoma cells. However, the action of nobiletin with regard to Aβ degradation under in vitro AD pathological conditions remains unclear. In this study, we examined whether nobiletin could enhance the degradation of intra- and extracellular Aβ using human induced pluripotent stem cell-derived AD model neurons, which generate an excess of Aβ due to the familial AD presenilin-1 mutation. The neurons were treated in the presence or absence of nobiletin. The results of real-time quantitative RT-PCR indicated that neprilysin mRNA levels were significantly up-regulated by nobiletin. Furthermore, immunostaining with an anti-Aβ antibody revealed that nobiletin substantially reduced the intraneuronal content of Aβ. Interestingly, the results of Aβ immunoassays confirmed that nobiletin also significantly decreased the levels of Aβ released into the cellular medium. These results suggest that nobiletin enhanced the reduction of intra- and that extracellular Aβ levels under AD pathologic conditions, and this is associated with the up-regulation of neprilysin expression. Collectively, nobiletin appears to be a promising novel prophylactic seed drug or functional food for AD.

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Section: Methodsmentioning

confidence: 99%

Nobiletin Reduces Intracellular and Extracellular β-Amyloid in iPS Cell-Derived Alzheimer’s Disease Model Neurons

Kimura

Shimizu

Koyama

et al. 2018

Biological & Pharmaceutical Bulletin

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Distribution and development of P2Y1-purinoceptors in the mouse retina

et al. 2013

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There is increasing evidence that ATP acts on purinergic receptors and mediates synaptic transmission in the retina. In a previous study, we raised the possibility that P2X-purinoceptors, presumably P2X(2)-purinoceptors in OFF-cholinergic amacrine cells, play a key role in the formation of OFF pathway-specific modulation. In this study, we examined whether the P2Y(1)-purinoceptors can function in cholinergic amacrine cells in the mouse retina since cholinergic amacrine cells in the rat retina express P2Y(1)-purinoceptors. P2Y(1)-purinoceptors were shown to be expressed in dendrites of both ON- and OFF-cholinergic amacrine cells in adults. At postnatal day 7, there was immunoreactivity for P2Y(1)-purinoceptors in the soma of cholinergic amacrine cells. At postnatal day 14, weak immunoreactivity for P2Y(1)-purinoceptors was detected in the dendrites but not in the soma of cholinergic amacrine cells. At postnatal day 21, strong immunoreactivity for P2Y(1)-purinoceptors was detected in dendrites of cholinergic amacrine cells. The expression pattern of P2Y(1)-purinoceptors was not affected by visual experience. We concluded that P2Y(1)-purinoceptors are not involved in the OFF-pathway-specific signal transmission in cholinergic amacrine cells of the mouse retina.

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Central leptin signaling deficiency induced by leptin receptor antagonist leads to hypothalamic proteomic remodeling

Mazuecos,

Artigas-Jerónimo,

Pintado

et al. 2024

Life Sciences

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Age and Visual Experience-dependent Expression of NMDAR1 Splice Variants in Rat Retina

Cited by 5 publications

References 69 publications

Nobiletin Reduces Intracellular and Extracellular β-Amyloid in iPS Cell-Derived Alzheimer’s Disease Model Neurons

Nobiletin Reduces Intracellular and Extracellular β-Amyloid in iPS Cell-Derived Alzheimer’s Disease Model Neurons

Distribution and development of P2Y1-purinoceptors in the mouse retina

Central leptin signaling deficiency induced by leptin receptor antagonist leads to hypothalamic proteomic remodeling

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