AGC kinases in plant development and defense

Hirt, Heribert; García, Ana Victoria Torres; Oelmüller, Ralf

doi:10.4161/psb.6.7.15580

Cited by 27 publications

(13 citation statements)

References 40 publications

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“…In addition, we identified the phosphoinositide‐specific phospholipase C, PLC2, as a putative target of AGC kinases. PLCs generate diacylglycerol that activates PKCs in animals and is rapidly phosphorylated into phosphatidic acid in plants, leading to the activation of AGC kinases . Of potential relevance in such pathway is AT4G32285, a protein active in phosphatidylinositol binding that might be phosphorylated by AGC kinases as well (Fig.…”

Section: Discussionmentioning

confidence: 99%

Phosphorylation dynamics of membrane proteins from Arabidopsis roots submitted to salt stress

et al. 2014

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An excess of NaCl in the soil is detrimental for plant growth. It interferes with mineral nutrition and water uptake and leads to accumulation of toxic ions in the plant. Understanding the response of roots to NaCl stress may facilitate the development of crops with increased tolerance to this and other stresses. Since controls achieved at the posttranslational level are of critical importance for regulating protein function, the present work used a robust label-free quantitative proteomic methodology to quantify phosphorylation events that affect root membrane proteins in Arabidopsis, in response to short-term (up to 2 h) NaCl treatments. This work identified 302 proteotypic phosphopeptides including 77 novel phosphorylated sites. NaCl treatment significantly altered the abundance of 74 phosphopeptides, giving novel insights into the regulation of major classes of membrane proteins, including ATPases, sodium transporters, and aquaporins. The data provide a unique access to phosphorylation reprogramming of ionic equilibrium in plant cells under NaCl stress. The use of predictive bioinformatic tools for kinase motifs suggested that root membrane proteins are substrates of cAMP-dependent protein kinase, cGMP-dependent protein kinase, and protein kinase C families, also called AGC kinases, arguing for an important role of lipid signaling in abiotic stress responses. It also pointed to cross-talks between protein kinase families during NaCl stress.

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Section: Discussionmentioning

confidence: 99%

Phosphorylation dynamics of membrane proteins from Arabidopsis roots submitted to salt stress

et al. 2014

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“…This attracts many researchers to use the positive effects of P. indica as a model system to study the molecular basis of mutualistic symbioses and symbiont-induced plant immunity (Qiang et al , 2012). Genetic and biochemical analyses uncovered possible signal transduction processes induced by P. indica in the root cells of host plants (Shahollari et al , 2005, 2007; Serfling et al , 2007; Vadassery et al , 2009 b ; Camehl et al , 2011; Hirt et al , 2011; Lee et al , 2011). Furthermore, Stein et al (2008) have shown that P. indica -induced resistance against powdery mildew requires jasmonic acid (JA), but no salicylic acid (SA) in Arabidopsis .…”

Section: Introductionmentioning

confidence: 99%

The maturation zone is an important target of Piriformospora indica in Chinese cabbage roots

She-qin

Zhi-hong

Chen

et al. 2013

Journal of Experimental Botany

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The mutualistic symbiont Piriformospora indica exhibits a great potential in agriculture. The interaction between P. indica and Chinese cabbage (Brassica campestris cv. Chinensis) results in growth and biomass promotion of the host plant and in particular in root hair development. The resulting highly bushy root phenotype of colonized Chinese cabbage seedlings differs substantially from reports of other plant species, which prompted the more detailed study of this symbiosis. A large-scale expressed sequence tag (EST) data set was obtained from a double-subtractive EST library, by subtracting the cDNAs of Chinese cabbage root tissue and of P. indica mycelium from those of P. indica-colonized root tissue. The analysis revealed ~700 unique genes rooted in 141 clusters and 559 singles. A total of 66% of the sequences could be annotated in the NCBI GenBank. Genes which are stimulated by P. indica are involved in various types of transport, carbohydrate metabolism, auxin signalling, cell wall metabolism, and root development, including the root hair-forming phosphoinositide phosphatase 4. For 20 key genes, induction by fungal colonization was confirmed kinetically during the interaction by real-time reverse transcription–PCR. Moreover, the auxin concentration increases transiently after exposure of the roots to P. indica. Microscopic analyses demonstrated that the development of the root maturation zone is the major target of P. indica in Chinese cabbage. Taken together, the symbiotic interaction between Chinese cabbage and P. indica is a novel model to study root growth promotion which, in turn, is important for agriculture and plant biotechnology.

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“…S3 A, B, and D, Table S2, and SI Materials and Methods) identified UCN as At1g51170 (Fig. 3A), encoding a member of the plant-specific AGC VIII family of protein kinases (22)(23)(24)(25). UCN is a functional kinase that can autophosphorylate in trans as well as transphosphorylate the general kinase substrate myelin basic protein (Fig.…”

Section: Ucn Restrains Growth In Various Lateral Organs and The Embryomentioning

confidence: 99%

Regulation of planar growth by the Arabidopsis AGC protein kinase UNICORN

Enugutti¹,

Kirchhelle²,

Oelschner³

et al. 2012

Proc. Natl. Acad. Sci. U.S.A.

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The spatial coordination of growth is of central importance for the regulation of plant tissue architecture. Individual layers, such as the epidermis, are clonally propagated and structurally maintained by symmetric cell divisions that are oriented along the plane of the layer. The developmental control of this process is poorly understood. The simple cellular basis and sheet-like structure of Arabidopsis integuments make them an attractive model system to address planar growth. Here we report on the characterization of the Arabidopsis UNICORN (UCN) gene. Analysis of ucn integuments reveals localized distortion of planar growth, eventually resulting in an ectopic multicellular protrusion. In addition, ucn mutants exhibit ectopic growth in filaments and petals, as well as aberrant embryogenesis. We further show that UCN encodes an active AGC VIII kinase. Genetic, biochemical, and cell biological data suggest that UCN suppresses ectopic growth in integuments by directly repressing the KANADI transcription factor ABERRANT TESTA SHAPE. Our findings indicate that UCN represents a unique plant growth regulator that maintains planar growth of integuments by repressing a developmental regulator involved in the control of early integument growth and polarity.AGC protein kinase | growth suppression | floral development | ovule | signal transduction P lant tissue morphogenesis depends on the coordination of cellular behavior within tissue layers. The formation of distinct layers depends on asymmetric cell division, the developmental control of which is under intense investigation (1, 2). After initiation, individual cell layers are propagated by symmetric cell divisions (3) with division planes often oriented along the plane of the layer (planar growth). For example, the layered structure of the epidermis is maintained by anticlinal cell divisions, whereas periclinal cell divisions are usually suppressed (4). Division planes of symmetrically dividing cells can be accurately predicted by a mathematical rule linking cell geometry and cytoskeletal dynamics (5), and much progress has been made in the elucidation of the cellular machinery controlling the division plane of dividing plant cells. However, developmental controls that maintain the planar orientation of symmetrical cell divisions within a tissue layer remain poorly understood (3).Arabidopsis integuments represent an attractive model to study tissue morphogenesis. They are lateral determinate tissues of the ovule, the progenitors of the seed coat, and undergo a regular mode of development resulting in simple tissue architecture. An inner and an outer integument originate from the epidermis of the central chalaza (6, 7). They grow into laminar extensions with each integument consisting of a bilayered sheet of anticlinally dividing cells. The outer integument eventually develops into a hood-like structure that envelops the distal nucellus carrying the developing embryo sac and the inner integument.Coordination of symmetrical cell divisions along the plane of the extendi...

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AGC kinases in plant development and defense

Cited by 27 publications

References 40 publications

Phosphorylation dynamics of membrane proteins from Arabidopsis roots submitted to salt stress

Phosphorylation dynamics of membrane proteins from Arabidopsis roots submitted to salt stress

The maturation zone is an important target of Piriformospora indica in Chinese cabbage roots

Regulation of planar growth by the Arabidopsis AGC protein kinase UNICORN

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