Agastache rugosa leaf extract inhibits the iNOS expression in ROS 17/2.8 cells activated with TNF-α and IL-1β

Abstract

It has been suggested that nitric oxide (NO) derived from inducible nitric oxide synthase (iNOS) may act as a mediator of cytokine-induced effects on bone turn-over. NO is also recognized as an important factor in bone remodeling, i.e., participating in osteoblast apoptosis in an arthritic joint. The components of Agastache rugosa are known to have many pharmacological activities. In the present study, we investigated the effects of Agastache rugosa leaf extract (ELAR) on NO production and the iNOS expression in ROS 17/2.8 cells activated by a mixture of inflammatory cytokines including TNF-alpha and IL-1beta. A preincubation with ELAR significantly and concentration-dependently reduced the expression of iNOS protein in ROS 17/2.8 cells activated with the cytokine mixture. Consequently, the NO production was also significantly reduced by ELAR with an IC50 of 0.75 mg/mL. The inhibitory mechanism of iNOS induction by ELAR prevented the activation and translocation of NF-kappaB (p65) to the nucleus from the cytosol fraction. Furthermore, ELAR concentration-dependently reduced the cellular toxicity induced by sodium nitroprusside, an NO-donor. These results suggest that ELAR may be beneficial in NO-mediated inflammatory conditions such as osteoporosis.

A Chinese Decoction, Kuan-Sin-Yin, Improves Autonomic Function and Cancer-Related Symptoms of Metastatic Colon Cancer

“…Each of the components has been shown to have pharmacological activity in modulating the immune system and to have anti-inflammatory or antioxidant properties or both. [16][17][18][19][20][21][22] The purity of the KSY was evaluated by screening ingredients for contamination by heavy metals and pesticides and by a microbial limit test. Standard contents and quality control were analyzed by Internal Transcribed Space (ITS) technology and high-performance liquid chromatography (HPLC) at the Industrial Technology Research Institute, Hsinchu, Taiwan.We used ITS to analyze each of the 7 component ingredients in KSY to establish their DNA sequence, then analyzed the final KSY decoction using HPLC-PCA.…”

Rosmarinic acid production in hairy root cultures of Agastache rugosa Kuntze

“…It is used in Chinese traditional medicine for the treatment of cholera, vomiting, and miasma, and has been reported to have antitumor, antifungal, antiatherogenic and cytotoxic activities (Oh et al 2005;Shin and Kang 2003;Hong et al 2001).…”

A Chinese Medicine, Kuan-Sin-Yin Decoction, Improves Liver Function in Hepatitis B Virus Carriers: A Randomized, Controlled Study

“…Previous in vitro and in vivo studies have shown that Codonopsis pilosula, Atractylodes macrocephala, Poria cocos, Glycyrrhiza, Radix astragali, Agastachis herba, and Fructus ligustri have the potential to modulate the immune system, with antiinflammatory and antioxidant properties. [17][18][19][20][21][22][23] Thus, KSY might have the potential to improve liver function through immune modulation, with anti-inflammation and antioxidant properties. The details of the mechanism must be further explored in the future.…”