Agar Plate Method Using Lactobacillus plantarum for Biotin Determination in Serum and Urine.

Fukui, Toru; Iinuma, Kazuso; Oizumi, Jun; Izumi, Yoshikazu

doi:10.3177/jnsv.40.491

Cited by 51 publications

(33 citation statements)

References 14 publications

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“…Biotin: Bound biotin in liver was converted to free form by autoclave under acidic conditions, and biotin in urine, plasma and liver were determined by the microbioassay method using Lactobacillus plantarum ATCC 8014 ( 21 ).…”

Section: Methodsmentioning

confidence: 99%

Age-Related Alterations of B-Group Vitamin Contents in Urine, Blood and Liver from Rats

Fukuwatari

Wada

Shibata

2008

J Nutr Sci Vitaminol

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SummaryTo investigate how aging alters B-group vitamin metabolism, rats were fed with niacin-free 20% casein diet from 3 to 80 wk old, and the urinary excretions of the B group vitamins were periodically measured. The blood and liver B-group vitamin levels in 80-wk-old rats were also compared with those in 8-wk-old rats. The urinary excretion of thiamin, riboflavin, vitamin B 6 metabolite 4-pyridoxic acid, pantothenic acid, folic acid and biotin were not altered during 540 d. The urinary vitamin B 12 increased by 8-fold at 29 wk old, and further increased at 80 wk old. Conversion of nicotinamide from tryptophan gradually decreased to 60% from 29 to 48 wk old. Plasma PLP, vitamin B 12 and folate levels in 80-wk-old rats were lower than those in 8-wk-old rats, consistent with lower liver vitamin B 6 and folate levels in aged rats. Plasma and liver biotin levels in aged rats were higher than those in young rats. Other B-group vitamins such as vitamin B 1 , vitamin B 2 , niacin and pantothenic acid levels in blood and liver from aged rats were same as those from young rats. Alteration of vitamin B 6 metabolism in particular is similar to the observations in eldery humans reported previously. Our findings suggest that aged rats can be useful models to investigate aging-related B-group vitamin metabolism. Key Words aging, vitamin B 2 , vitamin B 6 , folate, biomarker The precise cascade of pathological age-related events is still not clearly understood, but enhanced production of free radicals and its deleterious effects on macro-biomolecules such as proteins and DNA ( 1 , 2 ) as well as polyunsaturated fatty acids ( 3 ) are well known during aging. Aging is a major risk factor for various chronic disorders including cancer. It is well known that taking in nutrients suitable for life stages is important to prevent the aging process and chronic disorders. Food, namely nutrient intake, is a key factor affecting aging and the incidence of many chronic disorders ( 4 -6 ). Decreased food intake and a sedentary lifestyle reduce energy expenditure and may alter their metabolism in elderly adults, and alteration of metabolism may cause alteration of nutrient requirement. However, there is not sufficient information on how aging alters vitamin metabolism ( 7 , 8 ). To investigate age-related alterations of B-group vitamins, rats were kept for 540 d from 3 to 80 wk old, and the urinary excretions of the B group vitamins were periodically measured. The blood and liver B-group vitamin levels in 80-wk-old rats were also compared with those in 8-wk-old rats.

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Section: Methodsmentioning

confidence: 99%

Age-Related Alterations of B-Group Vitamin Contents in Urine, Blood and Liver from Rats

Fukuwatari

Wada

Shibata

2008

J Nutr Sci Vitaminol

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“…Biotin: Serum and urinary biotin were determined by the microbioassay method using Lactobacillus plantarum ATCC 8014 (16).…”

Section: Determination Of Vitamins and Their Metabolites In Blood Andmentioning

confidence: 99%

Intra- and Inter-Individual Variations of Blood and Urinary Water-Soluble Vitamins in Japanese Young Adults Consuming a Semi-Purified Diet for 7 Days

Shibata

Fukuwatari

Watanabe

et al. 2009

J Nutr Sci Vitaminol

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SummaryWe have previously reported the levels of water-soluble vitamins in the blood and urine of Japanese young adults. In the present paper, to assess the variations in these water-soluble vitamin markers during the above experiment, we comprehensively determined the intra-and inter-individual variations of blood and urinary water-soluble vitamins to exactly the same amount of water-soluble vitamin intakes in the same experiment. The blood samples before breakfast and the 24-h urine samples were periodically collected from Japanese college male ( n ϭ 10) and female ( n ϭ 10) students consuming a semi-purified diet with water-soluble vitamins based on Japanese Dietary Reference Intakes for 7 d, and the intra-and inter-individual variations of blood and urinary water-soluble vitamins or their metabolites in blood and urine samples after adaptation were calculated. Although urinary excretion of vitamin B 12 and vitamin C showed high intra-individual variations in both males and females, other urinary vitamins and all blood vitamins showed less than 20% of within-subject coefficients of variance in either male or female. Those showing more than 20% of between-subject coefficients of variances in both male and female were blood vitamin B 6 , vitamin B 12 and folate levels, and urinary vitamin B 1 , vitamin B 2 , vitamin B 12 , nicotinamide metabolites, pantothenic acid, biotin and vitamin C. These results showed that oral administration of constant of water-soluble vitamins generally decreased intra-individual variation, while individual differences in urinary vitamin excretion were observed. Key Words water-soluble vitamins, blood, urine, human study A decrease of the vitamin contents in the body causes a vitamin deficiency, and vitamin levels in blood and urine are low in that state. The concentration of nutrients is well controlled in healthy people, and their blood and urinary water-soluble vitamin levels are kept constant. Therefore, water-soluble vitamin contents in blood and urine are used to assess their status, and these indicators have also been used for estimating dietary reference intakes (DRIs) in the USA and Japan ( 1 , 2 ). The use of valid nutritional markers for watersoluble vitamins is helpful to evaluate one's nutritional status for the vitamins, and further assessment of water-soluble vitamin levels in blood and urine is important to use as more established nutritional markers. To determine water-soluble vitamin levels in the blood and urine of Japanese subjects, we have previously reported those values obtained from the last day of experiments in Japanese young adults consuming a semi-purified diet based on the Japanese Recommended Dietary Allowance for 7 d ( 3 ). Although the subjects took exactly the same amount of vitamins for consecutive 7 d and followed the same schedule through the experiment, their water-soluble vitamin contents in blood and urine were different by individual ( 3 ). It is well known that these biomarkers show some individual differences; however, how individuals respond ...

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“…The water used was 17-Mohm grade. Fukui et al (1994) previously developed an agar plate method using L. plantarum ATCC8014, a biotindependent microorganism. A small aliquot of samples was added to each well on the agar plate containing L. plantarum, BCP, and Biotin Assay Medium in a sterilized square plate, and the agar plate was incubated at 37°C for about 16 h. The diameter of the growth zone was measured and compared with the standard curve.…”

Section: Methodsmentioning

confidence: 99%

“…Biotin in natto has been measured (MEXT, 2015), but to the best of our knowledge the change of biotin level has not been examined in the natto making process (water soaking and fermentation of soybeans). Therefore, in this study, we monitored the change of biotin level during natto production by an agar plate bioassay using L. plantarum (Fukui et al, 1994;Muratsugu et al, 2017), and we detected biotinylated polypeptide in dry soybeans and Bacillus subtilis var. natto (Bacillus natto (Sawamura, 1913)).…”

Section: Introductionmentioning

confidence: 99%

Changes in biotin levels during production of natto, Japanese fermented soybean

Muratsugu

Washino

Onchi

et al. 2017

IJFAAS

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The change of biotin level during production of natto (Japanese fermented soybean) was investigated in this study. The total biotin level was measured by an agar plate bioassay using Lactobacillus plantarum ATCC 8014. The total biotin level decreased during water soaking, but increased after the fermentation of soybeans using Bacillus subtilis var. natto (B. natto) and reached a maximum level. The increase of total biotin was not affected by Asp, Arg, and Ile which promoted the growth of L. plantarum in high concentrations. The peak level of biotin in the fermented soybeans was significantly higher than that of dry soybeans. The fermented soybeans at the biotin peak level were adequate for food. In addition, we detected 9 and 4 biotinylated polypeptides in the soybeans and B. natto used in this study, respectively. We speculated that the increase of biotin level may depend on the increase of the 4 biotinylated polypeptides and free biotin in B. natto.

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Agar Plate Method Using Lactobacillus plantarum for Biotin Determination in Serum and Urine.

Cited by 51 publications

References 14 publications

Age-Related Alterations of B-Group Vitamin Contents in Urine, Blood and Liver from Rats

Age-Related Alterations of B-Group Vitamin Contents in Urine, Blood and Liver from Rats

Intra- and Inter-Individual Variations of Blood and Urinary Water-Soluble Vitamins in Japanese Young Adults Consuming a Semi-Purified Diet for 7 Days

Changes in biotin levels during production of natto, Japanese fermented soybean

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