Agar gel immunodiffusion test (AGID) evaluation for detection of bovine paratuberculosis in Rio de Janeiro, Brazil

Ferreira, Renata Marques; Fonseca, Leila de Souza; Lilenbaum, Walter

doi:10.1046/j.1472-765x.2002.01149.x

Cited by 21 publications

(15 citation statements)

References 11 publications

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“…This test is economical and mostly reliable in small ruminants. AGID test in unsatisfactory for subclinical cases due to low (Ferreira et al, 2002), but it gives reliable results in clinical cases (Mohan et al, 2013;RobbeAusterman et al, 2006).…”

Section: Agar Gel Immunodiffusion (Agid) Testmentioning

confidence: 99%

Epidemiology and Management Strategies of Johne's disease in Endemic Situations

Munir

Hassan

Muhammad³

et al. 2014

Res. j. vet. pract.

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Mycobacterium paratuberculosis the subspecies of M. avium, effects wide range of animals including domestic cattle, sheep, goats, buffaloes, camelids and wild ruminants resulting in progressive and chronic enteritis known as Johne's disease (paratuberculosis). Clinically sick animals show emaciation, diarrhea and eventually death but the risk is that mostly they don't show clinical sign still can shed bacteria in feces and milk. Organism spread in the animal body through blood and lymph nodes to multiple internal organs. It is economically very important disease in livestock because effected livestock is recommended to be culled due to high treatment costs. Etiology, host range, immunology, epidemiology, stages/ forms, clinical signs, diagnostic tools and treatment have been discussed with special reference to endemic situations. Strategies to control this disease include improved management practices, testing and culling and vaccination. Modifications in management practices is not an easy job and so is the case with testing and culling; vaccine on the other hand is the simple practice but it is not usually practiced by farmers because lack of knowledge/awareness in herdsmen and availability of vaccine.All copyrights reserved to Nexus® academic publishers

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Section: Agar Gel Immunodiffusion (Agid) Testmentioning

confidence: 99%

Epidemiology and Management Strategies of Johne's disease in Endemic Situations

Munir

Hassan

Muhammad³

et al. 2014

Res. j. vet. pract.

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“…Apesar de o ELISA ser o teste mais utilizado para o monitoramento da infecção em nível de rebanho, Ferreira et al (2002), Gomes et al (2002) e Ristow et al (2007 utilizaram o teste de AGID para estimar a prevalência da paratuberculose em rebanhos bovinos. Gomes et al (2002) relataram que o teste apresentou boa sensibilidade e especificidade para confirmação da infecção em bovinos com sinais clínicos da enfermidade, no entanto, o teste apresenta menor sensibilidade em relação ao ELISA, inclusive em pequenos ruminantes (Gumber et al 2006).…”

Section: Sorologiaunclassified

“…Gomes et al (2002) relataram que o teste apresentou boa sensibilidade e especificidade para confirmação da infecção em bovinos com sinais clínicos da enfermidade, no entanto, o teste apresenta menor sensibilidade em relação ao ELISA, inclusive em pequenos ruminantes (Gumber et al 2006). Outros pesquisadores brasileiros (Ferreira et al 2002, Ristow et al 2007) relataram baixa sensibilidade do teste AGID para bovinos.…”

Section: Sorologiaunclassified

Paratuberculose em ruminantes no Brasil

et al. 2013

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A paratuberculose ou doença de Johne é uma enterite granulomatosa causada por Mycobacterium avium subsp. paratuberculosis (Map) e comumente afeta ruminantes domésticos, no entanto, pode infectar várias espécies de mamíferos. Está presente nos cinco continentes e é considerada endêmica em algumas regiões pela Organização Internacional de Epizootias (OIE). Pertence à lista de enfermidades notificáveis, que compreende as doenças transmissíveis de importância sócio-econômica e/ou em saúde-pública, cujo controle é necessário para o comércio internacional de animais e alimentos de origem animal. A importância da doença de Johne não se restringe somente aos prejuízos econômicos causados à indústria animal, mas também na possível participação do Map na íleocolite granulomatosa que afeta seres humanos, conhecida como doença de Crohn. No Brasil, a paratuberculose já foi descrita em diversas espécies de ruminantes e em vários estados. Embora os relatos naturais da enfermidade sejam pontuais, acredita-se na possibilidade da transmissão interespecífica e na disseminação do agente através da compra e venda de animais infectados. O objetivo deste artigo foi reunir as informações disponíveis referentes aos aspectos epidemiológicos, clínico-patológicos e laboratoriais da paratuberculose em bovinos, bubalinos, caprinos e ovinos no Brasil, e salientar a necessidade de implementação de medidas de controle sanitário da enfermidade no país, o que possibilitaria a melhoria da qualidade e valorização dos produtos de origem animal no mercado internacional.

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“…Despite considerable research efforts aimed at development of better fecal processing and detection methods, the sensitivity of fecal cultures remains low. Serologic tests such as agar gel immunodiffusion (18), complement fixation (23), and enzyme-linked immunosorbent assay (ELISA) (9,15,23) are limited in their use because of both low specificity and sensitivity (7,15,47).…”

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confidence: 99%

Rapid Detection and Typing of Strains ofMycobacterium aviumsubsp.paratuberculosisfrom Broth Cultures

et al. 2005

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A liquid culture followed by molecular confirmation was evaluated for potential to improve sensitivity and reduce time to diagnosis of Mycobacterium avium subsp. paratuberculosis infection. Fecal samples from 240 animals from Ohio farms were assessed for presence of M. avium subsp. paratuberculosis using four different protocols: (i) sedimentation processing followed by inoculation on Herrold's Egg Yolk media (HEYM) slants (monitored biweekly up to 16 weeks), (ii) double centrifugation processing followed by inoculation on HEYM slants (monitored biweekly up to 16 weeks), (iii) liquid-solid double culture method using modified 7H9 broth (8 weeks) followed by subculture on HEYM slants (monitored up to 8 weeks), and (iv) liquid culture using modified 7H9 broth (8 weeks) followed by molecular assays for the presence of two M. avium subsp. paratuberculosis-specific targets. The number of positive samples detected by each protocol was 37, 53, 65, and 76, respectively. Twenty-seven samples were positive by all four methods. Based on samples positive by at least one method (n ‫؍‬ 81), the sensitivities for sedimentation processing, double centrifugation processing, liquid-solid double culture, and liquid culture followed by molecular confirmation were 46%, 65%, 80%, and 94%, respectively. Fingerprinting of the positive samples using two polymorphic (G and GGT) short sequence repeat regions identified varying levels of within-farm and between-farm diversity. Our data indicate that liquid culture followed by molecular confirmation can significantly improve sensitivity and reduce time-to-diagnosis (from 16 to 8 weeks) of M. avium subsp. paratuberculosis infection and can also be efficiently employed for the systematic differentiation of M. avium subsp. paratuberculosis strains to understand the epidemiology of Johne's disease.

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Agar gel immunodiffusion test (AGID) evaluation for detection of bovine paratuberculosis in Rio de Janeiro, Brazil

Cited by 21 publications

References 11 publications

Epidemiology and Management Strategies of Johne's disease in Endemic Situations

Epidemiology and Management Strategies of Johne's disease in Endemic Situations

Paratuberculose em ruminantes no Brasil

Rapid Detection and Typing of Strains ofMycobacterium aviumsubsp.paratuberculosisfrom Broth Cultures

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