Ag
            <sup>+</sup>
            Implantation Induces Mechanical properties, Cell Adhesion and Antibacterial Effects of TiN/Ag Multilayers
            <i>In Vitro</i>

Sun, Xiuhua; Gong, Huanhuan; Li, Dejun; Dong, Lei; Zhao, Mengli; Wan, Rongxin; Gu, Haitao

doi:10.2217/nnm-2017-0087

Cited by 11 publications

(10 citation statements)

References 32 publications

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“…Similar to cellular adhesion, the chemical properties of the surface of biomaterials also affect bacterial adhesion and aggregation. The TiN coating used in the present study has been reported to reduce bacterial adhesion [41,42], mainly due to antibacterial properties from the addition of N, as well as the topography and surface roughness [43]. The size of certain bacterial strains such as P. gingivalis is substantially smaller than that of cells, and their attachment may be affected by nanoroughness.…”

Section: Discussionmentioning

confidence: 93%

Biofunctionalization of Microgroove Surfaces with Antibacterial Nanocoatings

Lai¹,

Chen

et al. 2020

BioMed Research International

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Objectives. To investigate the physical properties of the modified microgroove (MG) and antibacterial nanocoated surfaces. In addition, the biological interactions of the modified surfaces with human gingival fibroblasts (HGFs) and the antibacterial activity of the surfaces against Porphyromonas gingivalis were studied. Methods. The titanium nitride (TiN) and silver (Ag) coatings were deposited onto the smooth and MG surfaces using magnetron sputtering. A smooth titanium surface (Ti-S) was used as the control. The physicochemical properties including surface morphology, roughness, and hydrophilicity were characterized using scanning electron microscopy, atomic force microscopy, and an optical contact angle analyzer. The “contact guidance” morphology was assessed using confocal laser scanning microscopy. Cell proliferation was analyzed using the Cell Counting Kit-8 assay. The expression level of the main focal adhesion-related structural protein vinculin was compared using quantitative reverse transcription PCR and Western blotting. The antibacterial activity against P. gingivalis was evaluated using the LIVE/DEAD BacLight™ Bacterial Viability Kit. Results. The Ag and TiN antibacterial nanocoatings were successfully deposited onto the smooth and MG surfaces using magnetron sputtering technology. TiN coating on a grooved surface (TiN-MG) resulted in less nanoroughness and greater surface hydrophilicity than Ag coating on a smooth surface (Ag-S), which was more hydrophobic. Cell proliferation and expression of vinculin were higher on the TiN-MG surface than on the Ag-coated surfaces. Ag-coated surfaces showed the strongest antibacterial activity, followed by TiN-coated surfaces. Conclusion. Nano-Ag coating resulted in good antimicrobial activity; however, the biocompatibility was questionable. TiN nanocoating on an MG surface showed antibacterial properties with an optimal biocompatibility and maintained the “contact guidance” effects for HGFs.

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Section: Discussionmentioning

confidence: 93%

Biofunctionalization of Microgroove Surfaces with Antibacterial Nanocoatings

Lai¹,

Chen

et al. 2020

BioMed Research International

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“…With the increase of total Zn and Ag coimplanted content, water angles of Zn/Ag(1/5)-TiN, Zn/Ag(1/2)-TiN, and Zn/ Ag(1/0.3)-TiN display a downward trend, which could be attributed to the fact that the increasing particles of Zn and Ag reduce the smoothness of the TiN surface to a greater extent. 25 Bovine serum albumin (BSA) is an important part of blood, which plays a role of nutrition provider and carrier, and it can promote cell adhesion. 26 The protein adsorption ability of various samples in BSA solution is shown in Figure 3b.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

Effects of Zn and Ag Ratio on Cell Adhesion and Antibacterial Properties of Zn/Ag Coimplanted TiN

Zhao

et al. 2019

ACS Biomater. Sci. Eng.

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Titanium alloy (Ti-6Al-4 V) has good biocompatibility and mechanical properties, but its biologically inert and poor antibacterial property limit its application in the field of medical implants. In this study, titanium nitride (TiN) is first deposited on the surface of titanium alloy to wrap toxic elements (for example, vanadium). Then Zn and Ag ions with different ratios are simultaneously implanted into TiN via a plasma immersion ion implantation (PIII) system. X-ray photoelectron spectrometer proves the presence of Zn, Ag, and TiN on the surface of Zn/Ag-TiN. Protein adsorption suggests that, with the increase of Zn ratio, the adsorption ability is enhanced. Biological assays indicate that Zn/Ag(1/0.3)-TiN has the best cell proliferation and adhesion, because it has a larger ratio of Zn. Zn/Ag(1/2)-TiN has the best antibacterial effect and followed by Zn/Ag(1/0.3)-TiN, since Ag plays a major role in antibacterial activity and Zn is auxiliary. But a large ratio of Ag not only impairs cells but also has a negative effect on antibacterial activity. In conclusion, Zn/Ag(1/0.3)-TiN offers both excellent cytocompatibility and antibacterial activity and has great potential in orthopedic implants.

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“…Biomedical titanium alloy (Ti‐6Al‐4 V) plates with a diameter of 10 mm were carefully polished and ultrasonically cleaned several times in ethanol and deionized water. A detailed description of the multilayer deposition and ion implantation has been presented in our previous study (Sun et al, 2016b; Sun et al, 2017). TiN/Ag multilayers were prepared using a multiarc ion‐plating system (SA‐6 T, China) and different doses of Ag ions were implanted on the surface of the TiN/Ag multilayers using an ion implantation system (MEVVA80III‐100, China).…”

Section: Methodsmentioning

confidence: 99%

“…In this study, Ag ion-incorporated TiN/Ag multilayers were fabricated on titanium alloys to achieve both immediate and long-term antibacterial activity. The data presented in our previous study, suggested that coating Ti-6Al-4 V with TiN/Ag multilayers using an appropriate Ag ion implantation dose improved crystallinity, hardness, elastic modulus, and resistance to wear (Sun et al, 2017). However, the osteogenesis of rat bone mesenchymal stem cells (BMSCs) and the long-term antibacterial activity of the Ag-implanted TiN/Ag multilayers are not well understood.…”

Section: Introductionmentioning

confidence: 99%

Study on the osteogenesis of rat mesenchymal stem cells and the long‐term antibacterial activity of Staphylococcus epidermidis on the surface of silver‐rich TiN/Ag modified titanium alloy

et al. 2020

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The main causes of failure of orthopedic implants are infection and poor bone ingrowth. Surface modification of the implants to allow for long‐term antibacterial and osteogenic functions is an effective solution to prevent failure of the implants. We developed silver‐rich TiN/Ag nano‐multilayers on the surface of titanium alloy with different doses of Ag+. The antibacterial stability and osteogenesis of the silver‐rich surface were determined by evaluating the adhesion and proliferation of Staphylococcus epidermidis, and the adhesion, proliferation, alkaline phosphatase activity, extracellular matrix mineralization, and the expression level of genes involved in osteogenic differentiation of rat bone mesenchymal stem cells (BMSCs). The results demonstrated that the antibacterial rates (Ra) of 5 × 1016‐Ag, 1 × 1017‐Ag, 5 × 1017‐Ag, and 1 × 1018‐Ag were respectively 46.21%, 85.66%, 94.99%, 98.48%, and 99.99%. After subcutaneous implantation in rats or immersion in phosphate buffered saline for up to 12 weeks, the silver‐rich surface of the titanium alloy showed long‐term stable inhibition of Staphylococcus epidermidis. Furthermore, in vitro and in vivo studies indicated that the Ag‐implanted titanium did not show apparent cytotoxicity and that lower Ag+ implanted groups (5 × 1016‐Ag, 1 × 1017‐Ag) had better viability and biological safety when compared with higher Ag+ implanted groups. In addition, when compared with the Ti6Al4V‐group, all Ag‐implanted groups exhibited enhanced osteogenic indicators in rat BMSCs. Regarding osteogenic indicators, the surfaces of the 5 × 1017‐Ag group had better osteogenic effects than those of other groups. Therefore, the proper dose of Ag+ implanted TiN/Ag nano‐multilayers may be one of the options for the hard tissue replacement materials with antibacterial activity and osteogenic functions.

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Ag ⁺ Implantation Induces Mechanical properties, Cell Adhesion and Antibacterial Effects of TiN/Ag Multilayers In Vitro

Abstract: Our results indicate that a dose of 1 × 1017 ions/cm induced an improvement in crystallinity, mechanical properties, as well as preferable cell adhesion and antibacterial effects.

Cited by 11 publications

References 32 publications

Biofunctionalization of Microgroove Surfaces with Antibacterial Nanocoatings

Biofunctionalization of Microgroove Surfaces with Antibacterial Nanocoatings

Effects of Zn and Ag Ratio on Cell Adhesion and Antibacterial Properties of Zn/Ag Coimplanted TiN

Study on the osteogenesis of rat mesenchymal stem cells and the long‐term antibacterial activity of Staphylococcus epidermidis on the surface of silver‐rich TiN/Ag modified titanium alloy

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Ag + Implantation Induces Mechanical properties, Cell Adhesion and Antibacterial Effects of TiN/Ag Multilayers In Vitro

Abstract: Our results indicate that a dose of 1 × 1017 ions/cm induced an improvement in crystallinity, mechanical properties, as well as preferable cell adhesion and antibacterial effects.

Cited by 11 publications

References 32 publications

Biofunctionalization of Microgroove Surfaces with Antibacterial Nanocoatings

Biofunctionalization of Microgroove Surfaces with Antibacterial Nanocoatings

Effects of Zn and Ag Ratio on Cell Adhesion and Antibacterial Properties of Zn/Ag Coimplanted TiN

Study on the osteogenesis of rat mesenchymal stem cells and the long‐term antibacterial activity of Staphylococcus epidermidis on the surface of silver‐rich TiN/Ag modified titanium alloy

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Ag ⁺ Implantation Induces Mechanical properties, Cell Adhesion and Antibacterial Effects of TiN/Ag Multilayers In Vitro