Ag₂[Fe(CN)₅NO] Nanoparticles Exhibit Antibacterial Activity and Wound Healing Properties

Abstract

Therapeutic agents harboring both wound healing and antibacterial activities have much demand in biomedical applications. Development of such candidates with clinically approved materials adds more advantages toward these applications. Recently, silver metal complex nanomaterials have been playing a major role in medical uses especially for antibacterial activity and wound healing. In this report, we designed and synthesized silver nitroprusside complex nanoparticles (abbreviated as AgNNPs) using sodium nitroprusside and silver nitrate (both are FDA approved precursors). The nanoparticles (AgNNPs) were thoroughly characterized by various physicochemical techniques such as XRD, FTIR, TGA, DLS, EDAX, Raman, ICP-OES, HRTEM, and FESEM. The cell viability assay in normal cells (EA.hy 926 cells, NIH 3T3) using MTT reagents and CEA assay (CEA: Chick embryo angiogenesis assay) in fertilized eggs demonstrate the biocompatibility of AgNNPs. These nanoparticles show effective antibacterial activity against both Gram positive and Gram negative bacteria through membrane and DNA damage. Additionally, AgNNPs accelerate the wound healing in C57BL6 mice by altering the macrophages from M1 to M2. Considering the results together, the current study may offer the development of new silver nanocomplex nanomaterials that shows synergistic effect on antibacterial activity and wound healing (2-in-1-system). To the best of our knowledge, this is the first report for the synthesis, characterization, and biomedical applications of silver nitroprusside nanoparticles.

Fabrication of Chitosan-Reinforced Multifunctional Graphene Nanocomposite as Antibacterial Scaffolds for Hemorrhage Control and Wound-Healing Application

“…The presence of silver has effectively accelerated the cell proliferation via restricting the inflammatory cells and removing the barrier during the re-epithelization process. 84,85 Adding to it, the involvement of graphene has provided an enhanced mechanical strength and ideal structural stability to the Cs-GAP100 nanobiocomposite film and prevented its easy degradation. It is also reported that graphene, silver, and polycationic peptide possess a remarkable antibacterial property that would further help in preventing the microbial growth at the wound site.…”

Ultrafine Silver Nanoparticles Embedded in Cyclodextrin Metal‐Organic Frameworks with GRGDS Functionalization to Promote Antibacterial and Wound Healing Application

“…The currently available silver‐based treatment for burns and wounds including silver carboxymethyl cellulose, silver alginate, silver sulfadiazine, etc., possess problems of less stability and toxicity in their native forms . The antibacterial properties of NPs are size dependent since only the ultrafine NPs with diameters of 1–10 nm present a preferential interaction with the bacteria .…”

Additively manufactured small‐diameter vascular grafts with improved tissue healing using a novel SNAP impregnation method

“…Despite the application of sterile surgical techniques, bacterial contamination is considered as a main clinical complication of current synthetic vascular grafts, especially for small‐diameter vascular grafts (SDVGs, <6 mm) which can cause graft failure (Kabirian, Amoabediny, Haghighipour, Salehi‐Nik, & Zandieh‐Doulabi, ; Kabirian, Ditkowski, Zamanian, Heying, & Mozafari, ; Pashneh‐Tala et al, ; Radakovic et al, ; Rao et al, ). Bacteria start to colonize immediately after implantation especially during the critical first 6 hr which is also known as the “decisive period.” Since the management of this period is directly related to success or failure of implantation and the host's defenses are not able to completely combat the infection process during this period (Zilberman & Elsner, ), antimicrobial agents are used in the form of systemic or controlled release systems.…”