Ag 5 IO 6 : novel antibiofilm activity of a silver compound with application to medical devices

Incani, Vanessa; Omar, Amin; Prosperi-Porta, Graeme; Nadworny, Patricia L.

doi:10.1016/j.ijantimicag.2014.09.008

Cited by 14 publications

(16 citation statements)

References 13 publications

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“… Neutralization/disaggregation of LPS Polymyxin (B and E), Gramicidin S, Sushi peptides, PMAP-23 177,180,262 7. Alteration of membrane permeabilization Lantibiotics (nisin, gallidermin), Lytic peptides (PTP-7), Sophorolipids, Polyhexamethylene biguanide, Chlorhexidine, Pentasilver hexaoxoiodate 179,186,191,192 8. Inhibition of cell division or cell survival Pyrrhocoricin, Microcin B17 194,198 9.…”

Section: Methods For Quantification and Structural Assessment Of Biofmentioning

confidence: 99%

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Strategies for combating bacterial biofilms: A focus on anti-biofilm agents and their mechanisms of action

et al. 2018

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Biofilm refers to the complex, sessile communities of microbes found either attached to a surface or buried firmly in an extracellular matrix as aggregates. The biofilm matrix surrounding bacteria makes them tolerant to harsh conditions and resistant to antibacterial treatments. Moreover, the biofilms are responsible for causing a broad range of chronic diseases and due to the emergence of antibiotic resistance in bacteria it has really become difficult to treat them with efficacy. Furthermore, the antibiotics available till date are ineffective for treating these biofilm related infections due to their higher values of minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC), which may result in in-vivo toxicity. Hence, it is critically important to design or screen anti-biofilm molecules that can effectively minimize and eradicate biofilm related infections. In the present article, we have highlighted the mechanism of biofilm formation with reference to different models and various methods used for biofilm detection. A major focus has been put on various anti-biofilm molecules discovered or tested till date which may include herbal active compounds, chelating agents, peptide antibiotics, lantibiotics and synthetic chemical compounds along with their structures, mechanism of action and their respective MICs, MBCs, minimum biofilm inhibitory concentrations (MBICs) as well as the half maximal inhibitory concentration (IC50) values available in the literature so far. Different mode of action of anti biofilm molecules addressed here are inhibition via interference in the quorum sensing pathways, adhesion mechanism, disruption of extracellular DNA, protein, lipopolysaccharides, exopolysaccharides and secondary messengers involved in various signaling pathways. From this study, we conclude that the molecules considered here might be used to treat biofilm-associated infections after significant structural modifications, thereby investigating its effective delivery in the host. It should also be ensured that minimum effective concentration of these molecules must be capable of eradicating biofilm infections with maximum potency without posing any adverse side effects on the host.

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Section: Methods For Quantification and Structural Assessment Of Biofmentioning

confidence: 99%

“…This compound may consider to be used as a potent antimicrobial agent for disinfecting medical devices like catheters, implants, ventilators and wound dressing. 192 …”

Section: Alteration Of Membrane Potential or Membrane Permeabilizatiomentioning

confidence: 99%

Strategies for combating bacterial biofilms: A focus on anti-biofilm agents and their mechanisms of action

et al. 2018

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“…Recently, the prevalence of antimicrobial drug resistance to antibiotics has been increasing therefore, the use of silver as a disinfectant is inevitable. Silver is now used in consumer products such as textiles, cosmetics, and medical instruments 10 – 12 in the form of nanoparticles, which are prepared by the chemical reduction of silver salts 13 . Furthermore, studies have documented its potential in water disinfection 14 – 17 .…”

Section: Introductionmentioning

confidence: 99%

In situ facile green synthesis of Ag–ZnO nanocomposites using Tetradenia riperia leaf extract and its antimicrobial efficacy on water disinfection

Mtavangu

Machunda

Bruggen

et al. 2022

Sci Rep

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In this work, Ag–ZnO nanocomposites were prepared by a green synthesis route using aqueous leaf extract of Tetradenia riperia and investigated for antibacterial activity against Escherichia coli and Staphylococcus aureus. To optimize the synthesis of the Ag–ZnO, the effects of precursor concentrations, pH, and temperatures were studied. The Ag–ZnO nanocomposites were characterized by XRD, ATR-FTIR, FESEM, and TEM. Results show that the concentration of 8% Ag, the temperature of 80 °C, and a pH of 7–8 were optimal for the synthesis of Ag–ZnO nanocomposites. The XRD analysis showed the decrease in particle size of Ag–ZnO from 23.6 to 14.8 nm with an increase in Ag concentrations, which was further supported by FESEM analysis. TEM image of 8% Ag provides more information on the coexistence of Ag on ZnO where an average particle size of 14.8 nm was determined. The ATR-FTIR analysis confirmed the presence of phenolic compounds, which work as reducing and stabilizing agents. The antimicrobial activity results show that Ag–ZnO nanocomposite demonstrated a higher antimicrobial potency on E. coli than on S. aureus. Therefore, Tetradenia riperia leaf extract is a viable route for the synthesis of Ag–ZnO nanocomposites to be used for various applications, including water disinfection.

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“…The plate was transferred to a sonicator and sonicated on high for 30 min to dislodge the biofilm. Colonies were counted to determine the biofilm density on the pegs (Biofilm Growth Check) [27]. Following the rinse step and after breaking off the pegs, the MBEC lid with biofilm growth was transferred to the prepared challenge plate (fluconazole: 16–2048 μg ml −1 ; caspofungin: 2–256 μg ml −1 ; and amphotericin B: 4–512 μg ml −1 , 2× concentrated) and incubated at 36 °C for 24 h. After the antifungal challenge, colonies were counted to determine the biofilm density on the pegs.…”

Section: Methodsmentioning

confidence: 99%

Effect of antifungal agents, lysozyme and human antimicrobial peptide LL-37 on clinical Candida isolates with high biofilm production

Chen

Lee

2021

Journal of Medical Microbiology

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Introduction. Candida species can form biofilms on tissues and medical devices, making them less susceptible to antifungal agents. Hypothesis/Gap Statement. Antifungal combination may be an effective strategy to fight against Candida biofilm. Aim. In this study, we investigated the in vitro activity of fluconazole, caspofungin and amphotericin B, alone and in combination, against 17 clinical Candida tropicalis and 6 Candida parapsilosis isolates with high biofilm formation. We also tested LL-37 and lysozyme for anti-biofilm activity against a selected C. tropicalis isolate. Methodology. Candida biofilms were prepared using the 96-well plate-based method. The minimum biofilm eradication concentrations were determined for single and combined antifungal drugs. The activity of LL-37 and lysozyme was determined by visual reading for planktonic cells and using the XTT assay for biofilms. Results. Under biofilm conditions, fluconazole plus caspofungin showed synergistic effects against 60.9% (14 of 23) of the tested isolates, including 70.6% of C. tropicalis [fractional inhibitory concentration index (FICI), 0.26–1.03] and 33.3% of C. parapsilosis (FICI, 0.04–2.03) isolates. Using this combination, no antagonism was observed. Amphotericin B plus caspofungin showed no effects against 78.3% (18 of 23) of the tested isolates. Amphotericin B plus fluconazole showed no effects against 65.2% (15 of 23) of the tested isolates and may have led to antagonism against 2 C. tropicalis and 2 C. parapsilosis isolates. LL-37 and lysozyme had no effect on biofilms of the selected C. tropicalis isolate. Conclusions. We found that fluconazole plus caspofungin led to a synergistic effect against C. tropicalis and C. parapsilosis biofilms. The efficacy of the antifungal combination therapies of the proposed schemes against biofilm-associated Candida infections requires careful and constant evaluation.

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Ag 5 IO 6 : novel antibiofilm activity of a silver compound with application to medical devices

Cited by 14 publications

References 13 publications

Strategies for combating bacterial biofilms: A focus on anti-biofilm agents and their mechanisms of action

Strategies for combating bacterial biofilms: A focus on anti-biofilm agents and their mechanisms of action

In situ facile green synthesis of Ag–ZnO nanocomposites using Tetradenia riperia leaf extract and its antimicrobial efficacy on water disinfection

Effect of antifungal agents, lysozyme and human antimicrobial peptide LL-37 on clinical Candida isolates with high biofilm production

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