Afzelin positively regulates melanogenesis through the p38 MAPK pathway

Jung, Eunsun; Kim, Jin Hee; Kim, Miok; Jang, Sung-Hee; Kang, Mingyeong; Oh, Sae Woong; Nho, Youn Hwa; Kang, Seung-Gul; Kim, Min Hee; Park, See‐Hyoung; Lee, Jongsung

doi:10.1016/j.cbi.2016.06.010

Cited by 29 publications

(15 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…ERK, JNK, and p38 are the major kinases mediated by several steps of activation or regulation of the MAPK pathway [33,34]. Changes in early expression of phosphorylated ERK are closely related to melanin production, and late activation of p-ERK activates phosphorylation of MITF at Ser 73, leading to ubiquitination and proteasome-mediated degradation of MITF, which is associated with the downregulation of melanogenesis [35][36][37][38]. JNK activation leads to cell differentiation, apoptosis, and melanin production in melanocytes, and p38 activation has been reported to be associated with pigmentation by increasing tyrosinase activity [39][40][41][42][43][44].…”

Section: Discussionmentioning

confidence: 99%

Morin Induces Melanogenesis via Activation of MAPK Signaling Pathways in B16F10 Mouse Melanoma Cells

Shin

Kim

et al. 2021

Molecules

View full text Add to dashboard Cite

Morin is a well-known flavonoid, and has been reported to have various properties, such as anti-cell death, antioxidant, and anti-inflammatory properties. Although studies on the biochemical and biological actions of morin have been reported, the melanin biosynthesis effects and molecular mechanisms are unknown. In this study, we first found that morin has the effect of enhancing melanin biosynthesis in B16F10 mouse melanoma cells, and analyzed the molecular mechanism. In this study, we examined the effects of morin on the melanin contents and tyrosinase activity, as well as the protein expression levels of the melanogenic enzymes TRP-1, TRP-2, and microphtalmia-associated transcription factor (MITF) in B16F10 mouse melanoma cells. Morin showed no cytotoxicity in the concentration range of 5–100 μM, and significantly increased the intracellular tyrosinase activity and melanin contents. In mechanism analysis, morin increased the protein expression of TRP-1, TRP-2, and MITF associated with melanogenesis. Furthermore, morin increased phosphorylated ERK and p38 at the early time, and decreased phosphorylated ERK after 12 h. The results suggest that morin enhances melanin synthesis through the MAPK signaling pathways in B16F10 mouse melanoma cells.

show abstract

Section: Discussionmentioning

confidence: 99%

Morin Induces Melanogenesis via Activation of MAPK Signaling Pathways in B16F10 Mouse Melanoma Cells

Shin

Kim

et al. 2021

Molecules

View full text Add to dashboard Cite

show abstract

“…Some flavonoids, such as Mul A, possess specific purgative effect, prompting seeds to be discharged as soon as possible, which allows them to avoid possible damage in the digestive system of animals, and achieve species transmission for the plant. Afz positively regulates melanin production and antagonizes UV-induced cell damage, which protects the seeds from exposure to sunlight [ 34 ].…”

Section: Resultsmentioning

confidence: 99%

Evaluation of the chemical proﬁle from four germplasms sources of Pruni Semen using UHPLC-LTQ-Orbitrap-MS and multivariate analyses

Zhao

Liu²,

Zhang³

et al. 2022

Journal of Pharmaceutical Analysis

View full text Add to dashboard Cite

“…20) Furthermore, afzelin protected melanocytes against H 2 O 2 -induced oxidative stress by activating the nuclear factor-E2-related factor 2-antioxidant response element (Nrf2-ARE) signaling pathway. 26) Afzelin also increased melanogenic activity by activating p38 mitogen-activated protein kinase (MAPK). 27) Those biological data suggest that afzelin may exert SPF enhancing effect in sunscreen formulation through photoprotective and anti-inflammatory activity.…”

Section: Discussionmentioning

confidence: 99%

Evaluating the Sun Protection Factor of Cosmetic Formulations Containing Afzelin

Kim

Shin

Ryu

et al. 2021

CHEMICAL & PHARMACEUTICAL BULLETIN

View full text Add to dashboard Cite

Exposure to UV radiation damages the skin and increases the risk of skin cancer. Sunscreen is used to protect the skin from the harmful effects of UV radiation. However, the chemical UV filters used in sunscreen can show toxicity and cause allergic reactions. A safe sunscreen that includes a lower content of chemical UV filters and exerts an excellent effect on UV protection needs to be developed. The objective of this study was to investigate whether the addition of afzelin to sunscreen could improve the sun protection factor (SPF). A synergistic effect between afzelin and organic sunscreen agents including padimate O and oxybenzone was confirmed. Interestingly, 100% in vitro SPF-boosting was observed when afzelin (0.05%) was applied with a standard SPF formulation containing organic sunscreens while afzelin alone had no contribution to the SPF. In vivo SPF analysis of the standard SPF formulation showed an SPF value of 13.3 that increased to 20.1 when supplemented with afzelin (0.05%). Additionally, afzelin showed no skin irritation in a human trial. These results suggest that afzelin is useful as a natural additive in sunscreen formulations and provides an SPF-boosting effect. Afzelin supplementation to the formulation showed the potential to reduce the use of synthetic photoprotectors, which could minimize the risk of synthetic agent toxicity.

show abstract

Afzelin positively regulates melanogenesis through the p38 MAPK pathway

Cited by 29 publications

References 28 publications

Morin Induces Melanogenesis via Activation of MAPK Signaling Pathways in B16F10 Mouse Melanoma Cells

Morin Induces Melanogenesis via Activation of MAPK Signaling Pathways in B16F10 Mouse Melanoma Cells

Evaluation of the chemical proﬁle from four germplasms sources of Pruni Semen using UHPLC-LTQ-Orbitrap-MS and multivariate analyses

Evaluating the Sun Protection Factor of Cosmetic Formulations Containing Afzelin

Contact Info

Product

Resources

About