Afyonkarahisar’da Sığır ve Buzağılardan İzole Edilen Escherichia coli O157:H7 Suşlarında İntimin ve Enterohemolizin Genlerinin Belirlenmesi

Abstract

SummaryThe aim of this study was to detect enterohemolysin (EhlyA) and intimin (eaeA) virulence genes of 14 Escherichia coli O157:H7 strains isolated from 457 fecal samples (237 calves and 220 cattle) by PCR. While EhlyA gene was determined in 13 (92.8%) strains, the eaeA gene was positive in 8 (57.1%) strains. Of the 8 eaeA genes, 4 (50.0%) were obtained from diarrheic calves, 2 (25.0%) from non-diarrheic calves, and 2 (25.0%) from healthy cattle. A total of 7 (50.0%) strains were determined to harbour both of the EhlyA and eaeA genes. This study confirmed that calves, especially diarrheic, and cattle are a reservoir of E. coli O157:H7 strains that may be pathogenic for human.

Volume 10, No. 1, 2021

“…1), 15 E. coli strains (8.02%) were confirmed as E. coli O157. This is nearly agreed with Kang et al (2004), Manna et al (2006), and Kuyucuoğlu et al (2011) who recovered E. coli O157 from diarrheic calves in percentages of 9.8%, 7.59% and 10.6%, respectively. On the other hand, Stenkamp-Strahm et al (2017) recovered E. coli O157 in a percentage of 0.25%.…”

“…coli O157 to the attaching and effacing lesion caused by Intimin and not by the production of Shiga toxins. The Enterohemolysin encoding gene (ehylA) was detected in 13.3% of the isolated strains, whereas Kang et al (2004) and Kuyucuoğlu et al (2011) detected this gene in 91.6% and 92.8% of E. coli O157 strains, respectively. Positive strains for blaSHV and blaTEM genes at 237 and 445bp, respectively.…”