The physical and bacteriological evaluation of raw landfill leachate from Gosa Landfill in FCT, Abuja, Nigeria was carried out in this study using standard procedures. The probable bacteria identified from the biochemical tests of isolated samples were Streptococcus pneumoniae with Pseudomonas aeruginosa and Staphylococcus aureus. However, molecular characterization involving sequencing and BLAST further confirmed the bacteria present. This study concludes that the bio filter produced from Coco peat and Ash affects both the physicochemical properties and bacteriological flora of raw landfill leachate through degradation.