Afrostyrax lepidophyllus extracts exhibit in vitro free radical scavenging, antioxidant potential and protective properties against liver enzymes ion mediated oxidative damage

Abstract

BackgroundSeveral studies described the phytochemical constituents of plants in relation with the free radical scavenging property and inhibition of lipid peroxidation. This study investigated the in vitro antioxidant property, and the protective effects of ethanolic and aqueous ethanol extract of the leaves and barks of Afrostyrax lepidophyllus (Huaceae) against ion mediated oxidative damages.MethodsFour extracts (ethanol and aqueous-ethanol) from the leaves and barks of A. lepidophyllus were used in this study. The total phenols content, the antiradical and antioxidant properties were determined using standard colorimetric methods.ResultsThe plant extracts had a significant scavenging potential on the 2,2-diphenyl-1-picrylhydrazyl (DPPH), hydroxyl (OH), nitrite oxide (NO) and 2,2-azinobis (3-ethylbenzthiazoline)-6-sulfonic acid (ABTS) radicals with the IC50 varied between 47 and 200 µg/mL depending on the part of plant and the type of extract. The ethanol extract of A. lepidophyllus bark (GEE) showed the highest polyphenolic (35.33 ± 0.29) and flavonoid (12.00 ± 0.14) content. All the tested extracts demonstrated a high protective potential with the increased of superoxide dismutase, catalase and peroxidase activities.ConclusionAfrostyrax lepidophyllus extracts exhibited higher antioxidant potential and significant protective potential on liver enzymes.Electronic supplementary materialThe online version of this article (doi:10.1186/s13104-015-1304-8) contains supplementary material, which is available to authorized users.

Organic and Mineral Composition of Seeds of Afrostyrax lepidophyllus Mildbr. and Evaluation of ROS Inhibition and Cytotoxicity of Isolated Compounds

“…This plant has a very strong onion-like or garlic smell and is believed to annihilate cancer and repulsive to poisonous animals like snakes and scorpions. It belongs to the Huaceae family and grows in the forests areas in the Republic of Congo, Nigeria, Ghana, Central African Republic and Cameroon [1,2]. The barks of this plant are used in food sauces, condiments, spices and flavorings.…”

“…The barks of this plant are used in food sauces, condiments, spices and flavorings. Medicinally it is used in treating venereal diseases, febrifuges, cough, worms, constipation, hernia, gastro-enteric diseases, convulsions, laxative, mouthwash and snake bites [1,3]. This plant has been shown to possess antioxidant activity, estrogenic properties, insecticidal and toxic effects on some insects [4][5][6].…”

“…Gas chromatography mass spectrometry (GC-MS) analysis of seed oils as well as tree barks of this plant showed the presence of sulfur containing compounds mainly thio-ethers with 2,4,5,7-tetrathiaoctane as major component, monoterpene hydrocarbons and phenylpropanoids amongst others [3,8]. Afroastyrax lepidophyllus has also been shown to contain polyphenols, flavonoids, tannins, glycosides, saponins, steroids, aromatic substances, alkaloids, afrostyraxides and anthocyanins [1,2,9]. The organic constituents are regarded to be responsible for the medicinal properties of most plants while few works attempt to study the medicinal activities of their inorganic constituents.…”

Zanthoxylum heitzii Modulates Ferric Nitrilotriacetate-Dependent Oxidative Alterations in Four Vital Organs: An In Vitro Organoprotective Model

“…In previous works, our group demonstrated the protective activities of different plants used as a spice in Cameroonian diet against iron-mediated oxidative damage on rat liver [ 8 – 10 ]. Our chemical analysis of those plant samples revealed a high amount in eugenol, apigenin, catechin, and quercetin in the studied extracts [ 11 , 12 ].…”

Modifications anatomiques après écorçage de trois espèces au sud-ouest Cameroun

“…Toutefois, les études de Van Andel (2012) prouvent que son écorce est aussi utilisée pour traiter les convulsions. L'écorce interne (figure 3) est utilisée dans les incisions et les morsures de serpent (Muganza et al, 2012 ;Moukette et al, 2015). Les écorces des tiges de cette espèce sont utilisées comme épices dans la cuisson des aliments, et nécessitent souvent l'abattage de l'arbre entier, étant donné que plusieurs organes (écorces, bois, fruits) sont souvent recherchés (Loubelo, 2012).…”