African swine fever virus structural protein p17 inhibits cGAS-STING signaling pathway through interacting with STING

Abstract

African swine fever (ASF) is highly contagious, causes high mortality in domestic and feral swine, and has a significant economic impact on the global swine industry due to the lack of a vaccine or an effective treatment. African swine fever virus (ASFV) encodes more than 150 polypeptides, which may have intricate and delicate interactions with the host for the benefit of the virus to evade the host’s defenses. However, currently, there is still a lack of information regarding the roles of the viral proteins in host cells. Here, our data demonstrated that the p17, encoded by D117L gene could suppress porcine cGAS-STING signaling pathway, exhibiting the inhibitions of TBK1 and IRF3 phosphorylations, downstream promoter activities, cellular mRNA transcriptions and ISG56 induction, and antiviral responses. Further, we found that p17 was located in endoplasmic reticulum (ER) and Golgi apparatus, and interacted with STING, perturbing it in the recruitment of TBK1 and IKKϵ Additionally, it appeared that the transmembrane domain (amino acids 39–59) of p17 could be required for interacting with STING and inhibiting cGAS-STING pathway. Taken together, p17 could inhibit the cGAS-STING pathway through its interaction with STING and interference with STING in the recruitment of TBK1 and IKKϵ

Development of Visual Detection of African Swine Fever Virus Using CRISPR/LwCas13a Lateral Flow Strip Based on Structural Protein Gene D117L

“…Further, only three articles have reported the Cas13a system for detecting ASFV, which all targeted the p72/B646L gene [20,22,23]. We recently established CRISPR/LbCas12a detection and RPA-Cas12a-LFS detection of ASFV by targeting the structural gene D117L gene [26]. As a conserved ASFV structural gene, D117L not only has multiple PAM sequences (TTTN) recognized by LbCas12a, but also has several PFS sequences recognized by LwCas13a.…”