African swine fever virus in Asia: Its rapid spread and potential threat to unaffected countries

Lu, Gang; Pan, Jialiang; Zhang, Guihong

doi:10.1016/j.jinf.2019.11.011

Cited by 36 publications

(48 citation statements)

References 7 publications

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“…ASFV have led to tremendous economic losses in the epidemic regions and pose huge challenges to other regions with large scale pig production, including Western Europe, the North America and Latin America [5,8,9]. Therefore, huge efforts worldwide have been put on for developing effective and safe ASF vaccines.…”

Section: Discussionmentioning

confidence: 99%

“…In this study, we developed and evaluated a duplex real-time PCR targeting the B646L gene and MGF505-2R gene. The sequences of primers and probes are highly conserved in the genotype II ASFVs, which are prevalent in Asia, rans-Caucasus region, Eastern Europe and Russian Federation [5,8,9]. The generation of two signals (FAM and VIC) indicated the infection of wide type ASFVs.…”

Section: Discussionmentioning

confidence: 99%

“…The genotype I was historically prevalent in Western Europe and Latin America and was successfully eradicated except Sardinia area in Italy by the mid-1990s [8]. The genotype II is epidemic in the trans-Caucasus region, Eastern Europe and Russian Federation since 2007 and was introduced into Asia in 2018 [9]. Especially in Asia, the outbreaks are going in many countries and appears far from to be effectively controlled [5,8,9].…”

Section: Introductionmentioning

confidence: 99%

“…The genotype II is epidemic in the trans-Caucasus region, Eastern Europe and Russian Federation since 2007 and was introduced into Asia in 2018 [9]. Especially in Asia, the outbreaks are going in many countries and appears far from to be effectively controlled [5,8,9].…”

Section: Introductionmentioning

confidence: 99%

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Development and Evaluation of Duplex TaqMan Real-Time PCR Assay for Detection and Differentiation of Wide-Type and MGF505 Gene-Deleted African Swine Fever Viruses

Guo

Li²,

Qiao

et al. 2020

Preprint

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Background: African swine fever (ASF) is the most important disease to the pigs and cause serious economic losses to the countries with large-scale swine production. Vaccines are recognized as the most useful tool to prevent and control ASF virus (ASFV) infection. Currently, the MGF505 and MGF360 gene-deleted ASFVs or combined with CD2v deletion were confirmed to be the most promising vaccine candidates. Thus, it is essential to develop a diagnosis method to discriminate wide-type strain from the vaccines used. Results: In this study, we established a duplex TaqMan real-time PCR based on the B646L gene and MGF505-2R gene. The sequence alignment showed that the targeted regions of primers and probes are highly conserved in the genotype II ASFVs. The duplex real-time assay can specifically detect B646L and MGF505-2R gene single or simultaneously without cross-reaction with other porcine viruses tested. The limit of detection was 5.8 copies and 3.0 copies for the standard plasmids containing B646L and MGF505-2R genes, respectively. Clinical samples were tested in parallel by duplex real-time PCR and a commercial ASFV detection kit. The detection results of these two assays against B646L gene were well consistent. Conclusion: We successfully developed and evaluated a duplex TaqMan real-time PCR method which can effectively distinguish the wide type and MGF505 gene-deleted ASFVs. It would be a useful tool for the clinical diagnosis and control of ASF.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Development and Evaluation of Duplex TaqMan Real-Time PCR Assay for Detection and Differentiation of Wide-Type and MGF505 Gene-Deleted African Swine Fever Viruses

Guo

Li²,

Qiao

et al. 2020

Preprint

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“…The emergence of Seneca Valley virus (SVV) and increased incidence of SVV-associated vesicular disease alarmed the swine industry in several countries, including the US, Brazil, China, Thailand, and Colombia (8). Finally, a deadly swine disease, caused by African Swine Fever virus (ASFV), that has been plaguing Africa for decades, has now spread to south eastern Europe and Asia, and has severely impacted the world's largest swine industries in China (9).…”

Section: Introductionmentioning

confidence: 99%

Editorial: Porcine Anti-Viral Immunity

Vlasova¹,

Butler

2020

Front. Immunol.

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The roles of 6K protein on Getah virus replication and pathogenicity

Meng,

Mou,

Zhang

et al. 2023

Journal of Medical Virology

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Alphavirus is a type of arbovirus that can infect both humans and animals. The amino acid sequence of the 6K protein, being one of the structural proteins of the alphavirus, is not conserved. Deletion of this protein will result in varying effects on different alphaviruses. Our study focuses on the function of the Getah virus (GETV) 6K protein in infected cells and mice. We successfully constructed infectious clone plasmids and created resulting viruses (rGETV and rGETV‐Δ6K). Our comprehensive microscopic analysis revealed that the 6 K protein mainly stays in the endoplasmic reticulum. In addition, rGETV‐Δ6K has lower thermal stability and sensitivity to temperature than GETV. Although the deletion of the 6K protein does not reduce virion production in ST cells, it affects the release of virions from host cells by inhibiting the process of E2 protein transportation to the plasma membrane. Subsequent in vivo testing demonstrated that neonatal mice infected with rGETV‐Δ6K had a lower virus content, less significant pathological changes in tissue slices, and milder disease than those infected with the wild‐type virus. Our results indicate that the 6K protein effectively reduces the viral titer by influencing the release of viral particles. Furthermore, the 6K protein play a role in the clinical manifestation of GETV disease.

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African swine fever virus in Asia: Its rapid spread and potential threat to unaffected countries

Cited by 36 publications

References 7 publications

Development and Evaluation of Duplex TaqMan Real-Time PCR Assay for Detection and Differentiation of Wide-Type and MGF505 Gene-Deleted African Swine Fever Viruses

Development and Evaluation of Duplex TaqMan Real-Time PCR Assay for Detection and Differentiation of Wide-Type and MGF505 Gene-Deleted African Swine Fever Viruses

Editorial: Porcine Anti-Viral Immunity

The roles of 6K protein on Getah virus replication and pathogenicity

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