AFM study shows prominent physical changes in elasticity and pericellular layer in human acute leukemic cells due to inadequate cell–cell communication

Guz, Nataliia; Patel, Sapan J.; Dokukin, Maxim; Clarkson, Bayard D.; Sokolov, Igor

doi:10.1088/0957-4484/27/49/494005

Cited by 12 publications

(9 citation statements)

References 73 publications

(156 reference statements)

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“…For a qualitative description of the “amount” of glycocalyx on the endothelium, the following product of brush length and grafting density can be used 50 : …”

Section: Analysis and Classification Of Indentation Curvesmentioning

confidence: 99%

AFM-based detection of glycocalyx degradation and endothelial stiffening in the db/db mouse model of diabetes

Targosz‐Korecka

Jaglarz

Malek-Zietek

et al. 2017

Sci Rep

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Degradation of the glycocalyx and stiffening of endothelium are important pathophysiological components of endothelial dysfunction. However, to our knowledge, these events have not been investigated in tandem in experimental diabetes. Here, the mechanical properties of the glycocalyx and endothelium in ex vivo mouse aorta were determined simultaneously in indentation experiments with an atomic force microscope (AFM) for diabetic db/db and control db/+ mice at ages of 11–19 weeks. To analyze highly heterogeneous aorta samples, we developed a tailored classification procedure of indentation data based on a bi-layer brush model supplemented with Hertz model for quantification of nanomechanics of endothelial regions with and without the glycocalyx surface. In db/db mice, marked endothelial stiffening and reduced glycocalyx coverage were present already in 11-week-old mice and persisted in older animals. In contrast, reduction of the effective glycocalyx length was progressive and was most pronounced in 19-week-old db/db mice. The reduction of the glycocalyx length correlated with an increasing level of glycated haemoglobin and decreased endothelial NO production. In conclusion, AFM nanoindentation analysis revealed that stiffening of endothelial cells and diminished glycocalyx coverage occurred in early diabetes and were followed by the reduction of the glycocalyx length that correlated with diabetes progression.

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“…For a qualitative description of the “amount” of glycocalyx on the endothelium, the following product of brush length and grafting density can be used 50 : …”

Section: Analysis and Classification Of Indentation Curvesmentioning

confidence: 99%

AFM-based detection of glycocalyx degradation and endothelial stiffening in the db/db mouse model of diabetes

Targosz‐Korecka

Jaglarz

Malek-Zietek

et al. 2017

Sci Rep

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“…For example, the tumor microenvironment is composed of blood and lymphatic vessels and a variety of nonmalignant host cells, including fibroblasts, monocytes, macrophages, mast cells, mesenchymal stem cells and so on [89], [90]. Recent studies have shown that cell-cell contacts have a significant effect on cell mechanics [91]- [93]. In 2014, Guo et al [91] applied AFM to investigate the mechanical properties of cells in three different microenvironments as related to cell-cell contacts, including isolated cells, cells residing on the periphery of a contiguous cell monolayer, and cells on the inside of a contiguous cell monolayer.…”

Section: Cell-cell Contactmentioning

confidence: 99%

“…2C. In 2016, Guz et al [93] investigated the effects of cell-cell communication on the elasticity of human acute leukemic cells. The Young's modulus of cells grown in low density (5000 cells/ml) and high density (2 × 10 5 cells/ml) was measured respectively, showing that the Young's modulus significantly decreased in high density than in low density.…”

Section: Cell-cell Contactmentioning

confidence: 99%

Atomic Force Microscopy in Characterizing Cell Mechanics for Biomedical Applications: A Review

Dang

Liu

et al. 2017

IEEE Trans.on Nanobioscience

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-Cell mechanics is a novel label-free biomarker for indicating cell states and pathological changes. The advent of atomic force microscopy (AFM) provides a powerful tool for quantifying the mechanical properties of single living cells in aqueous conditions. The wide use of AFM in characterizing cell mechanics in the past two decades has yielded remarkable novel insights in understanding the development and progression of certain diseases, such as cancer, showing the huge potential of cell mechanics for practical applications in the field of biomedicine. In this paper, we reviewed the utilization of AFM to characterize cell mechanics. First, the principle and method of AFM single-cell mechanical analysis was presented, along with the mechanical responses of cells to representative external stimuli measured by AFM. Next, the unique changes of cell mechanics in two types of physiological processes (stem cell differentiation, cancer metastasis) revealed by AFM were summarized. After that, the molecular mechanisms guiding cell mechanics were analyzed. Finally the challenges and future directions were discussed.

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“…Biomechanical properties of cancer cells have shown to be closely related with cancer pathology and metastasis state abnormalities 7, 8. It is also found that the biomechanical changes were correlated with the cell-cell communications 9 and microenvironment 10, 11. Understanding the biomechanical properties of tumor cells can not only help to elucidate the mechanisms behind disease progression, but also provide important information for cancer diagnosis and treatment 12, 13, 14.…”

Section: Introductionmentioning

confidence: 99%

Regional biomechanical imaging of liver cancer cells

Pei¹,

Chen²,

Wang³

et al. 2019

J. Cancer

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Liver cancer is one of the leading cancers, especially in developing countries. Understanding the biomechanical properties of the liver cancer cells can not only help to elucidate the mechanisms behind the cancer progression, but also provide important information for diagnosis and treatment. At the cellular level, we used well-established atomic force microscopy (AFM) techniques to characterize the heterogeneity of mechanical properties of two different types of human liver cancer cells and a normal liver cell line. Stiffness maps with a resolution of 128x128 were acquired for each cell. The distributions of the indentation moduli of the cells showed significant differences between cancerous cells and healthy controls. Significantly, the variability was even greater amongst different types of cancerous cells. Fitting of the histogram of the effective moduli using a normal distribution function showed the Bel7402 cells were stiffer than the normal cells while HepG2 cells were softer. Morphological analysis of the cell structures also showed a higher cytoskeleton content among the cancerous cells. Results provided a foundation for applying knowledge of cell stiffness heterogeneity to search for tissue-level, early-stage indicators of liver cancer.

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AFM study shows prominent physical changes in elasticity and pericellular layer in human acute leukemic cells due to inadequate cell–cell communication

Cited by 12 publications

References 73 publications

AFM-based detection of glycocalyx degradation and endothelial stiffening in the db/db mouse model of diabetes

AFM-based detection of glycocalyx degradation and endothelial stiffening in the db/db mouse model of diabetes

Atomic Force Microscopy in Characterizing Cell Mechanics for Biomedical Applications: A Review

Regional biomechanical imaging of liver cancer cells

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